Rabbit Anti-SMYD3 Polyclonal Antibody – Cat# MBS126276

Western blot analysis of extracts of various cell lines, using SMYD3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

SMYD3 Antibody – Rabbit Polyclonal

Name: SMYD3 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS126276
Price: 360 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS126276

This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

SMYD3, Polyclonal Antibody

Full Product Name

SMYD3 Polyclonal Antibody

Product Gene Name

anti-SMYD3 antibody

Product Synonym Names

KMT3E; ZMYND1; ZNFN3A1; bA74P14.1

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

428

OMIM

608783

3D Structure

ModBase 3D Structure for Q9H7B4

Species

Human

Immunogen

Recombinant protein of human SMYD3

Calculated Molecular Weight

42kDa

Product Categories/Family for anti-SMYD3 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-SMYD3 antibody

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Application Notes for anti-SMYD3 antibody

WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
IP: 1:20 - 1:50

Other Notes

Small volumes of anti-SMYD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMYD3 purchase

MBS126276 is an antibody targeting SMYD3. Also known as SMYD3 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SMYD3. It may not necessarily be applicable to this product.

NCBI GI #

212276523

NCBI GeneID

64754

NCBI Accession #

Q9H7B4.4

UniProt Primary Accession #

Q9H7B4

UniProt Secondary Accession #

Q86TL8; Q8N5Z6; Q96AI5; A8K0P0; B1AN38

UniProt Related Accession #

Q9H7B4

NCBI Official Full Name

Histone-lysine N-methyltransferase SMYD3

NCBI Official Synonym Full Names

SET and MYND domain containing 3

NCBI Official Symbol

SMYD3

NCBI Official Synonym Symbols

KMT3E; ZMYND1; ZNFN3A1; bA74P14.1

UniProt Protein Name

Histone-lysine N-methyltransferase SMYD3

UniProt Synonym Protein Names

SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1

Protein Family

Histone-lysine N-methyltransferase

UniProt Gene Name

SMYD3

UniProt Synonym Gene Names

ZMYND1; ZNFN3A1

UniProt Entry Name

SMYD3_HUMAN

NCBI Protein Information

histone-lysine N-methyltransferase SMYD3; bA74P14.1 (novel protein); zinc finger, MYND domain containing 1; SET and MYND domain-containing protein 3; zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1

UniProt Comments for SMYD3

SMYD3: Histone methyltransferase. Specifically methylates 'Lys- 4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. Belongs to the histone-lysine methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.43; Methyltransferase, protein lysine; Methyltransferase

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: metal ion binding; histone-lysine N-methyltransferase activity

Biological Process: nucleosome assembly; establishment and/or maintenance of chromatin architecture; establishment of protein localization; myotube cell development; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation

Research Articles on SMYD3

1. Mutational analyses revealed that the MYND-domain of SMYD3 and domain III of hepatitis C virus NS5A are required for the interaction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMYD3 antibody

Reference Product	PubMed Publications
MET antibody	>2 publications with SMYD3 and MET
HELZ antibody	>2 publications with SMYD3 and HELZ
RASSF1 antibody	>1 publications with SMYD3 and RASSF1

Pathways associated with anti-SMYD3 antibody

Products by Pathway	Pathway Diagram
Chromatin Modifying Enzymes Pathway antibodies	Chromatin Modifying Enzymes Pathway Diagram
Chromatin Organization Pathway antibodies	Chromatin Organization Pathway Diagram
PKMTs Methylate Histone Lysines Pathway antibodies	PKMTs Methylate Histone Lysines Pathway Diagram

Diseases associated with anti-SMYD3 antibody

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>14 publications with SMYD3 and Adenocarcinoma
Carcinoma, Hepatocellular Antibodies	>12 publications with SMYD3 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>10 publications with SMYD3 and Liver Neoplasms
Breast Neoplasms Antibodies	>9 publications with SMYD3 and Breast Neoplasms
Cell Transformation, Neoplastic Antibodies	>4 publications with SMYD3 and Cell Transformation, Neoplastic
Carcinoma, Squamous Cell Antibodies	>2 publications with SMYD3 and Carcinoma, Squamous Cell
Inflammation Antibodies	>2 publications with SMYD3 and Inflammation
Disease Models, Animal Antibodies	>2 publications with SMYD3 and Disease Models, Animal
Neoplasm Metastasis Antibodies	>2 publications with SMYD3 and Neoplasm Metastasis
Lung Diseases Antibodies	>2 publications with SMYD3 and Lung Diseases

Organs/Tissues associated with anti-SMYD3 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>15 publications with SMYD3 and Liver
Muscle Antibodies	>10 publications with SMYD3 and Muscle
Heart Antibodies	>7 publications with SMYD3 and Heart
Lung Antibodies	>6 publications with SMYD3 and Lung
Prostate Antibodies	>3 publications with SMYD3 and Prostate
Embryonic Tissue Antibodies	>2 publications with SMYD3 and Embryonic Tissue
Lymph Node Antibodies	>2 publications with SMYD3 and Lymph Node
Bone Marrow Antibodies	>1 publications with SMYD3 and Bone Marrow
Vascular Antibodies	>1 publications with SMYD3 and Vascular
Blood Antibodies	>1 publications with SMYD3 and Blood