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LEP Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS126282
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Leptin is a 16 kDa adipocytederived hormone that relays the status of the body's energy reserves to the hypothalamus, resulting in the suppression of appetite and modulation of energy expenditure (1,2). Leptin functions as a pleiotropic cytokine with multiple… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LEP, Polyclonal Antibody
Full Product Name
LEP Polyclonal Antibody
Product Gene Name
Product Synonym Names
LEP; LEP; OB; OBS; Leptin; Obese protein; Obesity factor;
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Purity/Purification
Affinity purification
Preparation and Storage
Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles.
Molecular Weight
19kDa
Chromosome Location
Chromosome: 7; NC_000007.13 (127881241..127897682). Location: 7q31.3
OMIM
164160
3D Structure
ModBase 3D Structure for P41159
Immunogen
A synthetic peptide of human LEP
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-LEP antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-LEP antibody
Western Blot (WB): 1:200-1:500
Immunohistochemistry (IHC): 1:50-1:100
Immunofluorescence (IF): 1:20-1:50
Flow Cytometry (FC/FACS): 1:20-1:50

Western Blot (WB) of anti-LEP antibody

Western blot analysis of extracts of mouse pancreas, using LEP antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Rabbit Anti-LEP Polyclonal Antibody – Cat# MBS126282

Product References and Citations for anti-LEP antibody

1. Faggioni, R. et al. (2001) FASEB J 15, 256571. 2. Park, J. et al. (2011) Endocr Rev 32, 55070. 3. Mancuso, P. et al. (2011) J Immunol 186, 108190. 4. Hausman, G.J. and Barb, C.R. (2010) Endocr Dev 19, 3144 5. Kanda, N. and Watanabe, S. (2008) Endocrinology 149, 518998. 6. Tanaka, M. et al. (2011) J Neurosci 31, 837380. 7. Feldman, D.E. et al. (2012) Proc Natl Acad Sci U S A 109, 82934.

Other Notes

Small volumes of anti-LEP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LEP purchase

MBS126282 is an antibody targeting LEP. Also known as LEP Polyclonal Antibody. Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LEP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
leptin
NCBI Official Synonym Full Names
leptin
NCBI Official Symbol
LEP
NCBI Official Synonym Symbols
OB; OBS; LEPD
UniProt Protein Name
Leptin
UniProt Synonym Protein Names
Obese protein; Obesity factor
UniProt Gene Name
LEP
UniProt Synonym Gene Names
OB; OBS
UniProt Entry Name
LEP_HUMAN
NCBI Protein Information
leptin; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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