Rabbit Anti-ADD1 Polyclonal Antibody – Cat# MBS126287

Western blot analysis of extracts of various cell lines, using ADD1 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ADD1 Antibody – Rabbit Polyclonal

Name: ADD1 Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS126287
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS126287

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

ADD1, Polyclonal Antibody

Full Product Name

ADD1 Polyclonal Antibody

Product Gene Name

anti-ADD1 antibody

Product Synonym Names

ADDA; MGC3339; MGC44427

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

737

OMIM

102680

3D Structure

ModBase 3D Structure for P35611

Species

Human

Immunogen

Recombinant protein of human ADD1

Calculated Molecular Weight

44kDa

Product Categories/Family for anti-ADD1 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-ADD1 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-ADD1 antibody

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Other Notes

Small volumes of anti-ADD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADD1 purchase

MBS126287 is an antibody targeting ADD1. Also known as ADD1 Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ADD1. It may not necessarily be applicable to this product.

NCBI GI #

12644231

NCBI GeneID

118

NCBI Accession #

P35611.2

UniProt Primary Accession #

P35611

UniProt Secondary Accession #

Q13734; Q14729; Q16156; Q86XM2; Q9UJB6; A2A3N8; A2A3P0; B4DI79; D3DVR3; D3DVR4; D3DVR5

UniProt Related Accession #

P35611

NCBI Official Full Name

Alpha-adducin

NCBI Official Synonym Full Names

adducin 1 (alpha)

NCBI Official Symbol

ADD1

NCBI Official Synonym Symbols

ADDA

UniProt Protein Name

Alpha-adducin

UniProt Synonym Protein Names

Erythrocyte adducin subunit alpha

Protein Family

Adenosine deaminase

UniProt Gene Name

ADD1

UniProt Synonym Gene Names

ADDA

UniProt Entry Name

ADDA_HUMAN

NCBI Protein Information

alpha-adducin; erythrocyte adducin alpha subunit

UniProt Comments for ADD1

ADD1: a cytoskeletal protein that promotes the assembly of the spectrin-actin network. Adducin is a heterodimeric protein that consists of related subunits. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Three alternatively-spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; F-actin capping protein complex; intermediate filament cytoskeleton; focal adhesion; perinuclear region of cytoplasm; postsynaptic density; dendritic spine; plasma membrane; nucleus; cytosol

Molecular Function: calmodulin binding; actin filament binding; protein homodimerization activity; spectrin binding; protein heterodimerization activity; T cell receptor binding; structural molecule activity; actin binding; transcription factor binding

Biological Process: actin filament bundle formation; positive regulation of protein binding; hemoglobin metabolic process; apoptosis; in utero embryonic development; unfolded protein response; multicellular organism growth; cell morphogenesis; positive regulation of endocytosis; positive regulation of angiogenesis; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; homeostasis of number of cells within a tissue; erythrocyte differentiation; actin cytoskeleton organization and biogenesis; cell volume homeostasis; barbed-end actin filament capping; cell structure disassembly during apoptosis

Disease: Hypertension, Essential

Research Articles on ADD1

1. Data indicate that adducin promotes adhesion by regulating desmosomes and is part of a protective pathway in pemphigus.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ADD1 antibody

Reference Product	PubMed Publications
ADD2 antibody	>15 publications with ADD1 and ADD2

Pathways associated with anti-ADD1 antibody

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptotic Cleavage Of Cellular Proteins Pathway antibodies	Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
Apoptotic Execution Phase Pathway antibodies	Apoptotic Execution Phase Pathway Diagram
Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies	Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
IRE1alpha Activates Chaperones Pathway antibodies	IRE1alpha Activates Chaperones Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Programmed Cell Death Pathway antibodies	Programmed Cell Death Pathway Diagram
Unfolded Protein Response (UPR) Pathway antibodies	Unfolded Protein Response (UPR) Pathway Diagram
XBP1(S) Activates Chaperone Genes Pathway antibodies	XBP1(S) Activates Chaperone Genes Pathway Diagram

Diseases associated with anti-ADD1 antibody

Disease Name	Pubmed Publications
Hypertension Antibodies	>70 publications with ADD1 and Hypertension
Heart Diseases Antibodies	>12 publications with ADD1 and Heart Diseases
Fatty Liver Antibodies	>12 publications with ADD1 and Fatty Liver
Nervous System Diseases Antibodies	>12 publications with ADD1 and Nervous System Diseases
Brain Diseases Antibodies	>11 publications with ADD1 and Brain Diseases
Kidney Diseases Antibodies	>5 publications with ADD1 and Kidney Diseases
Proteinuria Antibodies	>4 publications with ADD1 and Proteinuria
Congenital Abnormalities Antibodies	>4 publications with ADD1 and Congenital Abnormalities
Hemorrhage Antibodies	>3 publications with ADD1 and Hemorrhage
Hypertrophy Antibodies	>2 publications with ADD1 and Hypertrophy

Organs/Tissues associated with anti-ADD1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>67 publications with ADD1 and Blood
Adipose Tissue Antibodies	>31 publications with ADD1 and Adipose Tissue
Connective Tissue Antibodies	>22 publications with ADD1 and Connective Tissue
Liver Antibodies	>18 publications with ADD1 and Liver
Muscle Antibodies	>14 publications with ADD1 and Muscle
Heart Antibodies	>14 publications with ADD1 and Heart
Kidney Antibodies	>11 publications with ADD1 and Kidney
Brain Antibodies	>8 publications with ADD1 and Brain
Nerve Antibodies	>5 publications with ADD1 and Nerve
Bone Antibodies	>5 publications with ADD1 and Bone