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CDC42 Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS126295
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The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to… More details

$225 / 0.02 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CDC42, Antibody
Full Product Name
CDC42 Polyclonal Antibody
Product Gene Name
Product Synonym Names
CDC42; CDC42Hs; G25K; TKS
Isotype
IgG
Host
Rabbit
Form/Format
PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Purity/Purification
Affinity purification
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Molecular Weight
Observed MW: 21KDa
Calculated MW: 21kDa
OMIM
116952
3D Structure
ModBase 3D Structure for P60953
Species Reactivity/
Cross-Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-191 of human CDC42 (NP_001782.1).

Product Categories/Family for anti-CDC42 antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CDC42 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescene (IF)
Application Notes for anti-CDC42 antibody
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Western Blot of anti-CDC42 antibody

Western blot analysis of extracts of various cell lines, using CDC42 antibody (MBS126295) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Detection: ECL Basic Kit (RM00020).
Rabbit Anti-CDC42 Antibody – Cat# MBS126295

Immunohistochemistry (IHC) of anti-CDC42 antibody

Immunohistochemistry of paraffin-embedded rat testis using CDC42 antibody (MBS126295) at dilution of 1:100 (40x lens).
Rabbit Anti-CDC42 Antibody, Immunohistochemistry (IHC) – Cat# MBS126295

Immunofluorescence (IF) of anti-CDC42 antibody

Immunofluorescence analysis of C6 cells using CDC42 Rabbit pAb (MBS126295) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Rabbit Anti-CDC42 Antibody, Immunofluorescence (IF) – Cat# MBS126295

Immunoflurescence (IF) of anti-CDC42 antibody

Immunofluorescence analysis of NIH-3T3 cells using CDC42 Rabbit pAb (MBS126295) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Rabbit Anti-CDC42 Antibody, Immunoflurescence (IF) – Cat# MBS126295

Other Notes

Small volumes of anti-CDC42 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDC42 purchase

MBS126295 is an antibody targeting CDC42. Also known as CDC42 Polyclonal Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC42. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Cell division control protein 42 homolog
NCBI Official Synonym Full Names
cell division cycle 42
NCBI Official Symbol
CDC42
NCBI Official Synonym Symbols
G25K; CDC42Hs
UniProt Protein Name
Cell division control protein 42 homolog
UniProt Synonym Protein Names
G25K GTP-binding protein
Protein Family
UniProt Gene Name
CDC42
UniProt Entry Name
CDC42_HUMAN
NCBI Protein Information
cell division control protein 42 homolog; G25K GTP-binding protein; GTP-binding protein, 25kD; growth-regulating protein; GTP binding protein, 25kDa; small GTP binding protein CDC42; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))

UniProt Comments for CDC42

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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