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TFF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS126297
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Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not… More details

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Product Overview

Online Datasheet in PDF
Product Name
TFF1, Polyclonal Antibody
Full Product Name
TFF1 Polyclonal Antibody
Product Gene Name
anti-TFF1 antibody
Product Synonym Names
pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Molecular Weight
Observed MW: 13KDa
Calculated MW: 9kDa
OMIM
113710
3D Structure
ModBase 3D Structure for P04155
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-84 of human TFF1/pS2 (NP_003216.1).

Product Categories/Family for anti-TFF1 antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-TFF1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-TFF1 antibody
WB: 1:500 - 1:1000
IHC: 1:50 - 1:200

Western Blot (WB) of anti-TFF1 antibody

Western blot analysis of extracts of BT-474 cells, using TFF1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ( MBS128200 ) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Rabbit Anti-TFF1 Polyclonal Antibody – Cat# MBS126297

Western Blot (WB) of anti-TFF1 antibody

Western blot analysis of various lysates, using TFF1/pS2 antibody (MBS126297) at 1:710 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.
Exposure time: 60s.
Rabbit Anti-TFF1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS126297

Western Blot (WB) of anti-TFF1 antibody

Western blot analysis of Mouse stomach, using TFF1/pS2 antibody (MBS126297) at 1:710 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.
Exposure time: 90s
Rabbit Anti-TFF1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS126297

Immunohistochemistry (IHC) of anti-TFF1 antibody

Immunohistochemistry of paraffin-embedded human mammary cancer using TFF1 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-TFF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126297

Immunohistochemistry (IHC) of anti-TFF1 antibody

Immunohistochemistry of paraffin-embedded human liver cancer using TFF1 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-TFF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126297

Immunohistochemistry (IHC) of anti-TFF1 antibody

Immunohistochemistry of paraffin-embedded rat lung using TFF1 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-TFF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126297

Immunohistochemistry (IHC) of anti-TFF1 antibody

Immunohistochemistry of paraffin-embedded rat kidney using TFF1 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-TFF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126297

Immunohistochemistry (IHC) of anti-TFF1 antibody

Immunohistochemistry of paraffin-embedded mouse brain using TFF1 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-TFF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126297

Immunohistochemistry (IHC) of anti-TFF1 antibody

Immunohistochemistry of paraffin-embedded human colon carcinoma using TFF1 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-TFF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126297

Immunohistochemistry (IHC) of anti-TFF1 antibody

Immunohistochemistry of paraffin-embedded human colon carcinoma using TFF1 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-TFF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126297

Immunohistochemistry (IHC) of anti-TFF1 antibody

Immunohistochemistry of paraffin-embedded human stomach using TFF1 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-TFF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126297

Other Notes

Small volumes of anti-TFF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFF1 purchase

MBS126297 is an antibody targeting TFF1. Also known as TFF1 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TFF1. It may not necessarily be applicable to this product.

NCBI GI #
131127
NCBI GeneID
7031
NCBI Accession #
P04155.1
UniProt Primary Accession #
P04155
UniProt Related Accession #
P04155
NCBI Official Full Name
Trefoil factor 1
NCBI Official Synonym Full Names
trefoil factor 1
NCBI Official Symbol
TFF1
NCBI Official Synonym Symbols
pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
UniProt Protein Name
Trefoil factor 1
UniProt Synonym Protein Names
Breast cancer estrogen-inducible protein; PNR-2; Polypeptide P1.A; hP1.A; Protein pS2
Protein Family
Trefoil factor
UniProt Gene Name
TFF1
UniProt Synonym Gene Names
BCEI; PS2; hP1.A
UniProt Entry Name
TFF1_HUMAN
NCBI Protein Information
trefoil factor 1; protein pS2; polypeptide P1.A; gastrointestinal trefoil protein pS2; breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence

UniProt Comments for TFF1

TFF1: Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular space

Molecular Function: protein binding; growth factor activity

Biological Process: response to peptide hormone stimulus; negative regulation of cell proliferation; maintenance of gastrointestinal epithelium; carbohydrate metabolic process; digestion; cell differentiation; response to iron ion; response to estradiol stimulus
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Research Articles on TFF1

1. Determination of TFF1 status might identify patients at different risk for relapse and help in making decision on administering adjuvant therapy for early breast cancer patients during the first 3 years of follow-up.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TFF1 antibody

 Reference Product  PubMed Publications
 FOXA1 antibody  >14 publications with TFF1 and FOXA1
 MUC5B antibody  >11 publications with TFF1 and MUC5B
 ESR1 antibody  >9 publications with TFF1 and ESR1
 PCNA antibody  >7 publications with TFF1 and PCNA
 GKN2 antibody  >7 publications with TFF1 and GKN2
 NCOA1 antibody  >3 publications with TFF1 and NCOA1
 NCOA3 antibody  >3 publications with TFF1 and NCOA3
 NCOA2 antibody  >2 publications with TFF1 and NCOA2

Pathways associated with anti-TFF1 antibody

 Products by Pathway  Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram

Diseases associated with anti-TFF1 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >407 publications with TFF1 and Breast Neoplasms
 Adenocarcinoma Antibodies  >135 publications with TFF1 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >73 publications with TFF1 and Neoplasm Metastasis
 Inflammation Antibodies  >41 publications with TFF1 and Inflammation
 Neoplasm Invasiveness Antibodies  >37 publications with TFF1 and Neoplasm Invasiveness
 Hyperplasia Antibodies  >34 publications with TFF1 and Hyperplasia
 Neoplasms, Experimental Antibodies  >31 publications with TFF1 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >22 publications with TFF1 and Cell Transformation, Neoplastic
 Precancerous Conditions Antibodies  >18 publications with TFF1 and Precancerous Conditions
 Lung Neoplasms Antibodies  >17 publications with TFF1 and Lung Neoplasms

Organs/Tissues associated with anti-TFF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >185 publications with TFF1 and Stomach
 Intestine Antibodies  >80 publications with TFF1 and Intestine
 Blood Antibodies  >66 publications with TFF1 and Blood
 Prostate Antibodies  >23 publications with TFF1 and Prostate
 Pancreas Antibodies  >18 publications with TFF1 and Pancreas
 Brain Antibodies  >15 publications with TFF1 and Brain
 Mammary Gland Antibodies  >11 publications with TFF1 and Mammary Gland
 Ovary Antibodies  >9 publications with TFF1 and Ovary
 Uncharacterized Tissue Antibodies  >1 publications with TFF1 and Uncharacterized Tissue
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