Rabbit Anti-DUSP1 Polyclonal Antibody – Cat# MBS126306

Western blot analysis of extracts of various cell lines, using DUSP1 Antibody at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.

DUSP1 Antibody – Rabbit Polyclonal

Name: DUSP1 Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS126306
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS126306

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has… More details

$225 / 0.02 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DUSP1, Polyclonal Antibody

Full Product Name

DUSP1 Polyclonal Antibody

Product Gene Name

anti-DUSP1 antibody

Product Synonym Names

HVH1; MKP1; CL100; MKP-1; PTPN10

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

367

OMIM

600714

3D Structure

ModBase 3D Structure for P28562

Species

Human

Route

Synthetic Peptide

Immunogen

A synthetic peptide of human DUSP1

Calculated Molecular Weight

39kDa

Product Categories/Family for anti-DUSP1 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-DUSP1 antibody

Western Blot (WB)

Application Notes for anti-DUSP1 antibody

WB: 1:500 - 1:2000

Other Notes

Small volumes of anti-DUSP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DUSP1 purchase

MBS126306 is an antibody targeting DUSP1. Also known as DUSP1 Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DUSP1. It may not necessarily be applicable to this product.

NCBI GI #

1346900

NCBI GeneID

1843

NCBI Accession #

P28562.3

UniProt Primary Accession #

P28562

UniProt Secondary Accession #

Q2V508; D3DQL9

UniProt Related Accession #

P28562

NCBI Official Full Name

Dual specificity protein phosphatase 1

NCBI Official Synonym Full Names

dual specificity phosphatase 1

NCBI Official Symbol

DUSP1

NCBI Official Synonym Symbols

HVH1; MKP1; CL100; MKP-1; PTPN10

UniProt Protein Name

Dual specificity protein phosphatase 1

UniProt Synonym Protein Names

Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase CL100

Protein Family

Dual specificity protein phosphatase

UniProt Gene Name

DUSP1

UniProt Synonym Gene Names

CL100; MKP1; PTPN10; VH1; MAP kinase phosphatase 1; MKP-1

UniProt Entry Name

DUS1_HUMAN

NCBI Protein Information

dual specificity protein phosphatase 1; MAP kinase phosphatase 1; protein-tyrosine phosphatase CL100; dual specificity protein phosphatase hVH1; serine/threonine specific protein phosphatase; mitogen-activated protein kinase phosphatase 1

UniProt Comments for DUSP1

MKP-1: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP1 specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation. It is induced in human skin fibroblasts by oxidative/heat stress and growth factors. Contains 1 rhodanese domain.

Protein type: Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cytoplasm; nucleus

Molecular Function: protein tyrosine/threonine phosphatase activity; protein binding; non-membrane spanning protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: negative regulation of MAP kinase activity; response to light stimulus; response to retinoic acid; response to cAMP; negative regulation of MAPKKK cascade; positive regulation of apoptosis; response to glucocorticoid stimulus; negative regulation of meiotic cell cycle; response to testosterone stimulus; protein amino acid dephosphorylation; response to estradiol stimulus; cellular response to hormone stimulus; regulation of apoptosis; response to hydrogen peroxide; endoderm formation; response to oxidative stress; response to calcium ion; negative regulation of apoptosis; inactivation of MAPK activity

Research Articles on DUSP1

1. MKP-1 and MKP-2 stability is regulated by ERK-mediated phosphorylation through a degradation pathway independent of polyubiquitination

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DUSP1 antibody

Reference Product	PubMed Publications
JUN antibody	>208 publications with DUSP1 and JUN
MAPK1 antibody	>117 publications with DUSP1 and MAPK1
MAPK3 antibody	>98 publications with DUSP1 and MAPK3
FOS antibody	>58 publications with DUSP1 and FOS
EGR1 antibody	>13 publications with DUSP1 and EGR1
MAPK8 antibody	>6 publications with DUSP1 and MAPK8
MAPK14 antibody	>5 publications with DUSP1 and MAPK14
MAPK12 antibody	>1 publications with DUSP1 and MAPK12

Pathways associated with anti-DUSP1 antibody

Products by Pathway	Pathway Diagram
ATF-2 Transcription Factor Network Pathway antibodies	ATF-2 Transcription Factor Network Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
ErbB1 Downstream Signaling Pathway antibodies	ErbB1 Downstream Signaling Pathway Diagram
Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies	Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
Regulation Of P38-alpha And P38-beta Pathway antibodies	Regulation Of P38-alpha And P38-beta Pathway Diagram
Serotonergic Synapse Pathway antibodies	Serotonergic Synapse Pathway Diagram

Diseases associated with anti-DUSP1 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>112 publications with DUSP1 and Inflammation
Necrosis Antibodies	>90 publications with DUSP1 and Necrosis
Lung Diseases Antibodies	>40 publications with DUSP1 and Lung Diseases
Cardiovascular Diseases Antibodies	>37 publications with DUSP1 and Cardiovascular Diseases
Nervous System Diseases Antibodies	>32 publications with DUSP1 and Nervous System Diseases
Liver Diseases Antibodies	>29 publications with DUSP1 and Liver Diseases
Breast Neoplasms Antibodies	>29 publications with DUSP1 and Breast Neoplasms
Hypertension Antibodies	>26 publications with DUSP1 and Hypertension
Neoplasms, Experimental Antibodies	>25 publications with DUSP1 and Neoplasms, Experimental
Carcinoma, Hepatocellular Antibodies	>19 publications with DUSP1 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-DUSP1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>101 publications with DUSP1 and Blood
Muscle Antibodies	>83 publications with DUSP1 and Muscle
Brain Antibodies	>74 publications with DUSP1 and Brain
Vascular Antibodies	>74 publications with DUSP1 and Vascular
Lung Antibodies	>61 publications with DUSP1 and Lung
Bone Antibodies	>50 publications with DUSP1 and Bone
Liver Antibodies	>49 publications with DUSP1 and Liver
Heart Antibodies	>44 publications with DUSP1 and Heart
Nerve Antibodies	>30 publications with DUSP1 and Nerve
Kidney Antibodies	>29 publications with DUSP1 and Kidney