EGFR Antibody – Rabbit Polyclonal

The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). cSrc is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCgamma binds at phosphoTyr992, resulting in activation of PLCgammamediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein cCbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phosphoTyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxyterminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).

1. Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 1849. 2. Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 40812. 3. Cooper, J.A. and Howell, B. (1993) Cell 73, 10514. 4. Hubbard, S.R. et al. (1994) Nature 372, 74654. 5. Biscardi, J.S. et al. (1999) J Biol Chem 274, 833543. 6. Emlet, D.R. et al. (1997) J Biol Chem 272, 407986. 7. Levkowitz, G. et al. (1999) Mol Cell 4, 102940. 8. Ettenberg, S.A. et al. (1999) Oncogene 18, 185566. 9. Rojas, M. et al. (1996) J Biol Chem 271, 2745661. 10. Feinmesser, R.L. et al. (1999) J Biol Chem 274, 1616873.

Small volumes of anti-EGFR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS126363 is an antibody targeting EGFR. Also known as EGFR Polyclonal Antibody. Reactive in Human, mouse, rat. Host: Rabbit.