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ITGAL Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS126471
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ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte… More details

$225 / 0.02 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ITGAL, Polyclonal Antibody
Full Product Name
ITGAL Polyclonal Antibody
Product Gene Name
anti-ITGAL antibody
Product Synonym Names
CD11A; LFA-1; LFA1A
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH 7.3.
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Molecular Weight
Observed MW: 180kDa
Calculated MW: 129kDa
OMIM
153370
3D Structure
ModBase 3D Structure for P20701
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 26-340 of human CD11a/LFA-1A/ITGAL (NP_001107852.1).

Product Categories/Family for anti-ITGAL antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ITGAL antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-ITGAL antibody
WB: 1:100 - 1:500
IHC-P: 1:50 - 1:100
IF/ICC: 1:50 - 1:100

Western Blot (WB) of anti-ITGAL antibody

Western blot analysis of extracts of various cell lines, using ITGAL antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Rabbit Anti-ITGAL Polyclonal Antibody – Cat# MBS126471

Western Blot (WB) of anti-ITGAL antibody

Western blot analysis of various lysates, using CD11a/LFA-1A/ITGAL Rabbit pAb (MBS126471) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25?g per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
Rabbit Anti-ITGAL Polyclonal Antibody, Western Blot (WB) – Cat# MBS126471

Immunohistochemistry (IHC) of anti-ITGAL antibody

Immunohistochemistry analysis of paraffin-embedded rat spleen using CD11a/LFA-1A/ITGAL Rabbit pAb (MBS126471) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Rabbit Anti-ITGAL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126471

Immunohistochemistry (IHC) of anti-ITGAL antibody

Immunohistochemistry analysis of paraffin-embedded rat spleen using CD11a/LFA-1A/ITGAL Rabbit pAb (MBS126471) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Rabbit Anti-ITGAL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126471

Immunohistochemistry (IHC) of anti-ITGAL antibody

Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD11a/LFA-1A/ITGAL Rabbit pAb (MBS126471) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Rabbit Anti-ITGAL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126471

Immunofluorescence (IF) of anti-ITGAL antibody

Immunofluorescence analysis of paraffin-embedded rat spleen using CD11a/LFA-1A/ITGAL Rabbit pAb (MBS126471) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Rabbit Anti-ITGAL Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS126471

Immunofluorescence (IF) of anti-ITGAL antibody

Confocal immunofluorescence analysis of THP-1 cells using CD11a/LFA-1A/ITGAL Rabbit pAb (MBS126471) at dilution of 1:200. Blue: DAPI for nuclear staining.
Rabbit Anti-ITGAL Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS126471

Other Notes

Small volumes of anti-ITGAL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ITGAL purchase

MBS126471 is an antibody targeting ITGAL. Also known as ITGAL Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ITGAL. It may not necessarily be applicable to this product.

NCBI GI #
88911345
NCBI GeneID
3683
NCBI Accession #
P20701.3
UniProt Primary Accession #
P20701
UniProt Secondary Accession #
O43746; Q45H73; Q96HB1; Q9UBC8
UniProt Related Accession #
P20701
NCBI Official Full Name
Integrin alpha-L
NCBI Official Synonym Full Names
integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
NCBI Official Symbol
ITGAL
NCBI Official Synonym Symbols
CD11A; LFA-1; LFA1A
UniProt Protein Name
Integrin alpha-L
UniProt Synonym Protein Names
CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA-1A; Leukocyte function-associated molecule 1 alpha chain; CD_antigen: CD11a
Protein Family
Integrin
UniProt Gene Name
ITGAL
UniProt Synonym Gene Names
CD11A; LFA-1A
UniProt Entry Name
ITAL_HUMAN
NCBI Protein Information
integrin alpha-L; LFA-1A; LFA-1 alpha; integrin gene promoter; CD11 antigen-like family member A; lymphocyte function-associated antigen 1; leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide

UniProt Comments for ITGAL

ITGAL: Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Belongs to the integrin alpha chain family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Cell surface

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; membrane; plasma membrane; immunological synapse; intercellular junction; integrin complex; external side of plasma membrane

Molecular Function: protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; ICAM-3 receptor activity; cell adhesion molecule binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; regulation of immune response; cell-matrix adhesion; activated T cell proliferation; positive regulation of calcium-mediated signaling; signal transduction; receptor clustering; heterophilic cell adhesion; leukocyte adhesion; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; positive regulation of T cell proliferation; inflammatory response; blood coagulation; cell adhesion; cell motility; leukocyte migration
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Research Articles on ITGAL

1. ITGAL is overexpressed in systemic lupus erythematosus CD4+ T cells, but expression is not linked to the typical clinical and serological parameters associated with the disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ITGAL antibody

 Reference Product  PubMed Publications
 ITGB2 antibody  >1095 publications with ITGAL and ITGB2
 ICAM1 antibody  >474 publications with ITGAL and ICAM1
 PTPRC antibody  >194 publications with ITGAL and PTPRC
 ICAM2 antibody  >41 publications with ITGAL and ICAM2
 ICAM3 antibody  >37 publications with ITGAL and ICAM3
 ITGAD antibody  >12 publications with ITGAL and ITGAD
 ICAM4 antibody  >4 publications with ITGAL and ICAM4
 CD226 antibody  >3 publications with ITGAL and CD226
 ICAM5 antibody  >2 publications with ITGAL and ICAM5

Pathways associated with anti-ITGAL antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 CXCR3-mediated Signaling Events Pathway antibodies  CXCR3-mediated Signaling Events Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with anti-ITGAL antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >244 publications with ITGAL and Inflammation
 Necrosis Antibodies  >218 publications with ITGAL and Necrosis
 Lung Diseases Antibodies  >69 publications with ITGAL and Lung Diseases
 Neoplasms, Experimental Antibodies  >35 publications with ITGAL and Neoplasms, Experimental
 Heart Diseases Antibodies  >26 publications with ITGAL and Heart Diseases
 Kidney Diseases Antibodies  >25 publications with ITGAL and Kidney Diseases
 Atherosclerosis Antibodies  >24 publications with ITGAL and Atherosclerosis
 Colitis Antibodies  >21 publications with ITGAL and Colitis
 Fibrosis Antibodies  >15 publications with ITGAL and Fibrosis
 Leukemia, Promyelocytic, Acute Antibodies  >15 publications with ITGAL and Leukemia, Promyelocytic, Acute

Organs/Tissues associated with anti-ITGAL antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1562 publications with ITGAL and Blood
 Bone Marrow Antibodies  >183 publications with ITGAL and Bone Marrow
 Lung Antibodies  >117 publications with ITGAL and Lung
 Lymph Antibodies  >86 publications with ITGAL and Lymph
 Lymph Node Antibodies  >78 publications with ITGAL and Lymph Node
 Spleen Antibodies  >76 publications with ITGAL and Spleen
 Intestine Antibodies  >53 publications with ITGAL and Intestine
 Brain Antibodies  >50 publications with ITGAL and Brain
 Kidney Antibodies  >48 publications with ITGAL and Kidney
 Liver Antibodies  >47 publications with ITGAL and Liver
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