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Glucose-6-Phosphate 1-Dehydrogenase (zwf) E Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1265107

MBS1265107 is a recombinant protein targeting zwf. Also known as Recombinant E Coli Glucose-6-phosphate 1-dehydrogenase. Host: E Coli. Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone. More details

$455 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Glucose-6-phosphate 1-dehydrogenase (zwf), Recombinant Protein
Full Product Name
Recombinant E Coli Glucose-6-phosphate 1-dehydrogenase
Product Gene Name
Sequence
MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRS
Host
E Coli
Form/Format
Liquid containing glycerol
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Molecular Weight
82 kDa
Sequence Positions
1-486aa; Partial
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P0AC53
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for zwf recombinant protein

Molecular characterization of the Escherichia coli K-12 zwf gene encoding glucose 6-phosphate dehydrogenase.Rowley D.L., Wolf R.E. Jr.J. Bacteriol. 173:968-977(1991) A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.DNA Res. 3:379-392(1996) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Clonal divergence in Escherichia coli as a result of recombination, not mutation.Guttman D.S., Dykhuizen D.E.Science 266:1380-1383(1994) Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway.Carter A.T., Pearson B.M., Dickinson J.R., Lancashire W.E.Gene 130:155-156(1993) Escherichia coli proteome analysis using the gene-protein database.VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.Electrophoresis 18:1243-1251(1997) Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate.Zhao J., Baba T., Mori H., Shimizu K.Metab. Eng. 6:164-174(2004) A linear pentapeptide is a quorum-sensing factor required for mazEF-mediated cell death in Escherichia coli.Kolodkin-Gal I., Hazan R., Gaathon A., Carmeli S., Engelberg-Kulka H.Science 318:652-655(2007) The extracellular death factor physiological and genetic factors influencing its production and response in Escherichia coli.Kolodkin-Gal I., Engelberg-Kulka H.J. Bacteriol. 190:3169-3175(2008)

Other Notes

Small volumes of zwf recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for zwf. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
glucose-6-phosphate 1-dehydrogenase
NCBI Official Symbol
zwf
NCBI Official Synonym Symbols
ECK1853; JW1841
UniProt Protein Name
Glucose-6-phosphate 1-dehydrogenase
UniProt Gene Name
zwf
UniProt Synonym Gene Names
G6PD; EDF
UniProt Entry Name
G6PD_ECOLI
NCBI Protein Information
glucose-6-phosphate 1-dehydrogenase

NCBI Summary for zwf

Residues 199-203 of zwf encode a NNWDN peptide involved in ChpA(MazF) toxicity that is likely converted to NNWNN by AsnA; a zwf deletion prevents NNWNN activity (Kolodkin-Gal, 2007). [More information is available at EcoGene: EG11221]. A null mutation in the zwf gene encoding glucose 6-phosphate-1-dehydrogenase does not affect the growth rate significantly. [More information is available at EcoCyc: EG11221].
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UniProt Comments for zwf

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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