Ba(e Coli-K12) KHG/KDPG Aldolase (eda) Recombinant Protein

Involved in the degradation of glucose via the Entner-Doudoroff pathway. Catalyzes the reversible, stereospecific retro-aldol cleavage of 2-Keto-3-deoxy-6-phosphogluconate (KDPG) to pyruvate and D-glyceraldehyde-3-phosphate. In the synthetic direction, it catalyzes the addition of pyruvate to electrophilic aldehydes with si-facial selectivity. It accepts some nucleophiles other than pyruvate, including 2-oxobutanoate, phenylpyruvate, and fluorobutanoate. It has a preference for the S-configuration at C2 of the electrophile.

The complete amino acid sequence and identification of the active-site arginine peptide of Escherichia coli 2-keto-4-hydroxyglutarate aldolase.Vlahos C.J., Dekker E.E.J. Biol. Chem. 263:11683-11691(1988) Cloning, nucleotide sequence, overexpression, and inactivation of the Escherichia coli 2-keto-4-hydroxyglutarate aldolase gene.Patil R.V., Dekker E.E.J. Bacteriol. 174:102-107(1992) Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli sequence analysis and localization of promoters for the edd-eda operon.Egan S.E., Fliege R., Tong S., Shibata A., Wolf R.E. Jr., Conway T.J. Bacteriol. 174:4638-4646(1992) Purine and one-carbon metabolism in Escherichia coli K12 DNA sequence of a second GAR transformylase.Smith J.M., Nygaard P. Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway.Carter A.T., Pearson B.M., Dickinson J.R., Lancashire W.E.Gene 130:155-156(1993) A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.DNA Res. 3:379-392(1996) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Active-site residues of 2-keto-4-hydroxyglutarate aldolase from Escherichia coli. Bromopyruvate inactivation and labeling of glutamate 45.Vlahos C.J., Dekker E.E.J. Biol. Chem. 265:20384-20389(1990) Escherichia coli proteome analysis using the gene-protein database.VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.Electrophoresis 18:1243-1251(1997) Characterization and crystal structure of Escherichia coli KDPGal aldolase.Walters M.J., Srikannathasan V., McEwan A.R., Naismith J.H., Fierke C.A., Toone E.J.Bioorg. Med. Chem. 16:710-720(2008) Covalent intermediate trapped in 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase structure at 1.95-A resolution.Allard J., Grochulski P., Sygusch J.Proc. Natl. Acad. Sci. U.S.A. 98:3679-3684(2001) Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli.Wymer N., Buchanan L.V., Henderson D., Mehta N., Botting C.H., Pocivavsek L., Fierke C.A., Toone E.J., Naismith J.H.Structure 9:1-9(2001) Mechanism of the Class I KDPG aldolase.Fullerton S.W., Griffiths J.S., Merkel A.B., Cheriyan M., Wymer N.J., Hutchins M.J., Fierke C.A., Toone E.J., Naismith J.H.Bioorg. Med. Chem. 14:3002-3010(2006)

Small volumes of eda recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1265232 is a recombinant protein targeting eda. Also known as Recombinant Ba(E Coli-k12) KHG/KDPG aldolase. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.