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Thermus Aquacicus DNA Polymerase I, Thermostable (pol1) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1265456

MBS1265456 is a recombinant protein targeting pol1. Also known as Recombinant thermus aquacicus DNA polymerase I, thermostable. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$315 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Thermus aquacicus DNA polymerase I, thermostable (pol1), Recombinant Protein
Full Product Name
Recombinant thermus aquacicus DNA polymerase I, thermostable
Product Gene Name
Product Synonym Gene Name
polA
Product Synonym Names
Taq polymerase 1
Sequence
LLESPKALEE APWPPPEGAF VGFVLSRKEP MWADLLALAA ARGGRVHRAP EPYKALRDLK EARGLLAKDL SVLALREGLG LPPGDDPMLL AYLLDPSNTT PEGVARRYGG EWTEEAGERA ALSERLFANL WGRLEGEERL LWLYREVERP LSAVLAHMEA TGVRLDVAYL RALSLEVAEE IARLEAEVFR LAGHPFNLNS RDQLERVLFD ELGLPAIGKT EKTGKRSTSA AVLEALREAH PIVEKILQYR ELTKLKSTYI DPLPDLIHPR TGRLHTRFNQ TATATGRLSS SDPNLQNIPV RTPLGQRIRR AFIAEEGWLL VALDYSQIEL RVLAHLSGDE NLIRVFQEGR DIHTETASWM FGVPREAVDP LMRRAAKTIN FGVLYGMSAH RLSQELAIPY EEAQAFIERY FQSFPKVRAW IEKTLEEGRR RGYVETLFGR RRYVPDLEAR VKSVREAAER MAFNMPVQGT AADLMKLAMV KLFPRLEEMG ARMLLQVHDE LVLEAPKERA EAVARLAKEV MEGVYPLAVP LEVEVGIGED WLSAKE
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Liquid or Lyophilized powder
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Molecular Weight
88.5 kDa
Sequence Positions
287-832aa; Partial
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P19821
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for pol1 recombinant protein

Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus.Lawyer F.C., Stoffel S., Saiki R.K., Myambo K., Drummond R., Gelfand D.H.J. Biol. Chem. 264:6427-6437(1989) Overproduction of Thermus aquaticus DNA polymerase and its structural analysis by ion-spray mass spectrometry.Ishino Y., Ueno T., Miyagi M., Uemori T., Imamura M., Tsunasawa S., Kato I.J. Biochem. 116:1019-1024(1994) Crystal structure of Thermus aquaticus DNA polymerase.Kim Y., Eom S.H., Wang J., Lee D.-S., Suh S.W., Steitz T.A.Nature 376:612-616(1995) Crystal structure of the large fragment of Thermus aquaticus DNA polymerase I at 2.5-A resolution structural basis for thermostability.Korolev S., Nayal M., Barnes W.M., di Cera E., Waksman G.Proc. Natl. Acad. Sci. U.S.A. 92:9264-9268(1995) Structure of Taq polymerase with DNA at the polymerase active site.Eom S.H., Wang J., Steitz T.A.Nature 382:278-281(1996) Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I structural basis for nucleotide incorporation.Li Y., Korolev S., Waksman G.EMBO J. 17:7514-7525(1998) Crystal structures of the Klenow fragment of Thermus aquaticus DNA polymerase I complexed with deoxyribonucleoside triphosphates.Li Y., Kong Y., Korolev S., Waksman G.Protein Sci. 7:1116-1123(1998) Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation.Li Y., Mitaxov V., Waksman G.Proc. Natl. Acad. Sci. U.S.A. 96:9491-9496(1999)

Other Notes

Small volumes of pol1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for pol1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
NCBI Official Full Name
DNA polymerase I, thermostable
UniProt Protein Name
DNA polymerase I, thermostable
UniProt Synonym Protein Names
Taq polymerase 1
Protein Family
UniProt Gene Name
polA
UniProt Synonym Gene Names
pol1
UniProt Entry Name
DPO1_THEAQ

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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