30s Ribosomal Protein S15 (rpsO) E Coli Recombinant Protein

One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA. In the E Coli 70S ribosome it has been modeled to contact the 23S rRNA of the 50S subunit forming part of bridge B4. In the two 3. 5 A resolved ribosome structures there are minor differences between side-chain conformations.

Nucleotide sequence of the gene for Escherichia coli ribosomal protein S15 (rpsO) .Takata R., Mukai T., Aoyagi M., Hori K.Mol. Gen. Genet. 197:225-229(1984) Expression of the rpsO and pnp genes structural analysis of a DNA fragment carrying their control regions.Portier C., Regnier P.Nucleic Acids Res. 12:6091-6102(1984) Promoter activity and transcript mapping in the regulatory region for genes encoding ribosomal protein S15 and polynucleotide phosphorylase of Escherichia coli.Evans S., Dennis P.P.Gene 40:15-22(1985) Nucleotide sequence of the pnp gene of Escherichia coli encoding polynucleotide phosphorylase. Homology of the primary structure of the protein with the RNA-binding domain of ribosomal protein S1.Regnier P., Grunberg-Manago M., Portier C.J. Biol. Chem. 262:63-68(1987) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Primary structure of the 16S rRNA binding protein S15 from Escherichia coli ribosomes.Morinaga T., Funatsu G., Funatsu M., Wittmann H.G.FEBS Lett. 64:307-309(1976) The existence of two genes between infB and rpsO in the Escherichia coli genome DNA sequencing and S1 nuclease mapping.Sands J.F., Regnier P., Cummings H.S., Grunberg-Manago M., Hershey J.W.B.Nucleic Acids Res. 16:10803-10816(1988) Escherichia coli proteome analysis using the gene-protein database.VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.Electrophoresis 18:1243-1251(1997) Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry.Arnold R.J., Reilly J.P.Anal. Biochem. 269:105-112(1999) All-atom homology model of the Escherichia coli 30S ribosomal subunit.Tung C.-S., Joseph S., Sanbonmatsu K.Y.Nat. Struct. Biol. 9:750-755(2002) Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement.Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.Cell 113:789-801(2003) Structures of the bacterial ribosome at 3.5 A resolution.Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D.Science 310:827-834(2005)

Small volumes of rpsO recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1265457 is a recombinant protein targeting rpsO. Also known as Recombinant E Coli 30S ribosomal protein S15. Host: E Coli.