CD40 Ligand (CD40LG) Human Recombinant Protein

Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.

Cloning of TRAP, a ligand for CD40 on human T cells.Graf D., Korthaeuer U., Mages H.W., Senger G., Kroczek R.A.Eur. J. Immunol. 22:3191-3194(1992) The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor expression of a soluble form of gp39 with B cell co-stimulatory activity.Hollenbaugh D., Grosmaire L.S., Kullas C.D., Chalupny J.N., Braesch-Andersen S., Noelle R.J., Stamenkovic I., Ledbetter J.A., Aruffo A.EMBO J. 11:4313-4321(1992) The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.Aruffo A., Farrington M., Hollenbaugh D., Li X., Milatovich A., Nonoyama S., Bajorath J., Grosmaire L.S., Stenkamp R., Neubauer M., Roberts R.L., Noelle R.J., Ledbetter J.A., Francke U., Ochs H.D.Cell 72:291-300(1993) Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion.Spriggs M.K., Armitage R.J., Strockbine L., Clifford K.N., Macduff B.M., Sato T.A., Maliszewski C.R., Fanslow W.C.J. Exp. Med. 176:1543-1550(1992) Human CD40-ligand molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production.Gauchat J.-F., Aubry J.-P., Mazzei G.J., Life P., Jomotte T., Elson G., Bonnefoy J.-Y.FEBS Lett. 315:259-266(1993) Structural organization of the gene for CD40 ligand molecular analysis for diagnosis of X-linked hyper-IgM syndrome.Shimadzu M., Nunoi H., Terasaki H., Ninomiya R., Iwata M., Kanegasaka S., Matsuda I.Biochim. Biophys. Acta 1260:67-72(1995) Human native soluble CD40L is a biologically active trimer, processed inside microsomes.Pietravalle F., Lecoanet-Henchoz S., Blasey H., Aubry J.-P., Elson G., Edgerton M.D., Bonnefoy J.-Y., Gauchat J.-F.J. Biol. Chem. 271:5965-5967(1996) Determination of carbohydrate structures N-linked to soluble CD154 and characterization of the interactions of CD40 with CD154 expressed in Pichia pastoris and Chinese hamster ovary cells.Khandekar S.S., Silverman C., Wells-Marani J., Bacon A.M., Birrell H., Brigham-Burke M., De;Marini D.J., Jonak Z.L., Camilleri P., Fishman-Lobell J.Protein Expr. Purif. 23:301-310(2001) Release of soluble CD40L from platelets is regulated by glycoprotein IIb/IIIa and actin polymerization.Furman M.I., Krueger L.A., Linden M.D., Barnard M.R., Frelinger A.L. III, Michelson A.D.J. Am. Coll. Cardiol. 43:2319-2325(2004) 2-A crystal structure of an extracellular fragment of human CD40 ligand.Karpsusas M., Hsu Y.-M., Wang J.-H., Thompson J., Lederman S., Chess L., Thomas D.Structure 3:1031-1039(1995) The role of polar interactions in the molecular recognition of CD40L with its receptor CD40.Singh J., Garber E., van Vlijmen H., Karpsusas M., Hsu Y.-M., Zheng Z., Naismith J.H., Thomas D.Protein Sci. 7:1124-1135(1998) Defective expression of T-cell CD40 ligand causes X-linked immunodeficiency with hyper-IgM.Korthaeuer U., Graf D., Mages H.W., Briere F., Padayachee M., Malcolm S., Ugazio A.G., Notarangelo L.D., Levinsky R.J., Kroczek R.A.Nature 361:539-541(1993) CD40 ligand mutations in X-linked immunodeficiency with hyper-IgM.Disanto J.P., Bonnefoy J.-Y., Gauchat J.-F., Fischer A., de Saint Basile G.Nature 361:541-543(1993) CD40 ligand gene defects responsible for X-linked hyper-IgM syndrome.Allen R.C., Armitage R.J., Conley M.E., Rosenblatt H., Jenkins N.A., Copeland N.G., Bedell M.A., Edelhoff S., Disteche C.M., Simoneaux D.K., Fanslow W.C., Belmont J.W., Spriggs M.K.Science 259:990-993(1993) Characterization of nine novel mutations in the CD40 ligand gene in patients with X-linked hyper IgM syndrome of various ancestry.Macchi P., Villa A., Strina D., Sacco M.G., Morali F., Brugnoni D., Giliani S., Mantuano E., Fasth A., Andersson B., Zegers B.J.M., Cavagni G., Reznick I., Levy J., Zan-Bar I., Porat Y., Airo P., Plebani A., Vezzoni P., Notarangelo L.D.Am. J. Hum. Genet. 56:898-906(1995) Signaling through CD40 rescues IgE but not IgG or IgA secretion in X-linked immunodeficiency with hyper-IgM.Saiki O., Tanaka T., Wada Y., Uda H., Inoue A., Katada Y., Izeki M., Iwata M., Nunoi H., Matsuda I.J. Clin. Invest. 95:510-514(1995) Mutation analysis in CD40 ligand deficiency leading to X-linked hypogammaglobulinemia with hyper IgM syndrome.Katz F., Hinshelwood S., Rutland P., Jones A., Kinnon C., Morgan G.3.0.CO;2-A>Hum. Mutat. 8:223-228(1996) A single strand conformation polymorphism study of CD40 ligand. Efficient mutation analysis and carrier detection for X-linked hyper IgM syndrome.Lin Q., Rohrer J., Allen R.C., Larche M., Greene J.M., Shigeoka A.O., Gatti R.A., Derauf D.C., Belmont J.W., Conley M.E.J. Clin. Invest. 97:196-201(1996) Mutations of the CD40 ligand gene in 13 Japanese patients with X-linked hyper-IgM syndrome.Nonoyama S., Shimadzu M., Toru H., Seyama K., Nunoi H., Neubauer M., Yata J., Och H.D.Hum. Genet. 99:624-627(1997) Mutations of the CD40 ligand gene and its effect on CD40 ligand expression in patients with X-linked hyper IgM syndrome.Seyama K., Nonoyama S., Gangsaas I., Hollenbaugh D., Pabst H.F., Aruffo A., Ochs H.D.Blood 92:2421-2434(1998)

Small volumes of CD40LG recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1265494 is a recombinant protein targeting CD40LG. Also known as Recombinant Human CD40 ligand. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.