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Flagellin (fliC) Escherichia Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1265520

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. More details

$405 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Flagellin (fliC), Recombinant Protein
Full Product Name
Recombinant Escherichia coli Flagellin (fliC)
Product Gene Name
Sequence
AQVINTNSLS LITQNNINKN QSALSSSIER LSSGLRINSA KDDAAGQAIA NRFTSNIKGL TQAARNANDG ISVAQTTEGA LSEINNNLQR VRELTVQATT GTNSESDLSS IQDEIKSRLD EIDRVSGQTQ FNGVNVLAKN GSMKIQVGAN DNQTITIDLK QIDAKTLGLD GFSVKNNDTV TTSAPVTAFG ATTTNNIKLT GITLSTEAAT DTGGTNPASI EGVYTDNGND YYAKITGGDN DGKYYAVTVA NDGTVTMATG ATANATVTDA NTTKATTITS GGTPVQIDNT AGSATANLGA VSLVKLQDSK GNDTDTYALK DTNGNLYAAD VNETTGAVSV KTITYTDSSG AASSPTAVKL GGDDGKTEVV DIDGKTYDSA DLNGGNLQTG LTAGGEALTA VANGKTTDPL KALDDAIASV DKFRSSLGAV QNRLDSAVTN LNNTTTNLSE AQSRIQDADY ATEVSNMSKA QIIQQAGNSV LAKANQVPQQ VLSLLQG
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Molecular Weight
67.2 kDa
Sequence Positions
2-498aa; Full Length of Mature Protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P04949
Production Note
Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to support@mybiosource.com for more details.

Product Categories/Family for fliC recombinant protein

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for fliC recombinant protein

Nucleotide sequence of the hag gene encoding flagellin of Escherichia coli.Kuwajima G., Asaka J., Fujiwara T., Fujiwara T., Node K., Kondo E.J. Bacteriol. 168:1479-1483(1986) Isolation and characterization of Escherichia coli hag operator mutants whose hag48 expression has become repressible by a Salmonella H1 repressor.Hanafusa T., Sakai A., Tominaga A., Enomoto M.Mol. Gen. Genet. 216:44-50(1989) A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.DNA Res. 3:379-392(1996) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) DNA sequence adjacent to flagellar genes and evolution of flagellar-phase variation.Szekely E., Simon M.J. Bacteriol. 155:74-81(1983) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.Link A.J., Robison K., Church G.M.Electrophoresis 18:1259-1313(1997) Protein identification with N and C-terminal sequence tags in proteome projects.Wilkins M.R., Gasteiger E., Tonella L., Ou K., Tyler M., Sanchez J.-C., Gooley A.A., Walsh B.J., Bairoch A., Appel R.D., Williams K.L., Hochstrasser D.F.J. Mol. Biol. 278:599-608(1998)

Other Notes

Small volumes of fliC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for fliC purchase

Bacterial cells have tails - scientists call them flagellin, which are composed of flagellin proteins like flagellin FLIC or FLAB. These cell projections in salmonella represent a major cell surface antigen eliciting immunogenic responses. Sequence analysis through genome workbench resources have uncovered common protein expression patterns between bacteria, allowing for cross reactivity of antigens and antibodies in human serum. To further this potential benefit, a recombinant FLIC protein is available for your immunology studies in the USA and beyond. Produced by BLAST confirmed sequences and highly purified, our quality is supported by Google Scholar and PMC free article (s) - view article PubMed searches for more information. By using the most up to date genes AMP expression systems and providing all technical data for this product, your experiments will be robust and reproducible. Order today with our promotional pricing and value sized quantities at MyBiosource.com.

Additional Details

NCBI/Uniprot data below describe general gene information for fliC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
GenBank Nucleotide #
NCBI Official Full Name
flagellar filament structural protein (flagellin)
NCBI Official Symbol
fliC
NCBI Official Synonym Symbols
ECK1922; flaF; H; hag; JW1908
UniProt Protein Name
Flagellin
Protein Family
UniProt Gene Name
fliC
UniProt Synonym Gene Names
flaF; hag
UniProt Entry Name
FLIC_ECOLI
NCBI Protein Information
flagellar filament structural protein (flagellin)

NCBI Summary for fliC

Flagellar regulon. [More information is available at EcoGene: EG10321]. FliC, or flagellin, is the basic subunit that polymerizes to form the rigid flagellar filament of Escherichia coli. [More information is available at EcoCyc: EG10321].

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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