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Nad(p)h-Flavin Reductase (fre) Escherichia Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1265545

Catalyzes the reduction of soluble flavins by reduced pyridine nucleotides. Ses to reduce the complexed Fe3+ iron of siderophores to Fe2+, thus releasing it from the chelator. More details

$405 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NAD(P)H-flavin reductase (fre), Recombinant Protein
Full Product Name
Recombinant Escherichia coli NAD(P)H-flavin reductase (fre)
Product Gene Name
fre recombinant protein
Product Synonym Names
Aquacobalamin reductase; FMN reductase; Ferrisiderophore reductase CNAD(P)H:flavin oxidoreductase; Riboflavin reductase [NAD(P)H]
Sequence
TTLSCKVTSV EAITDTVYRV RIVPDAAFSF RAGQYLMVVM DERDKRPFSM ASTPDEKGFI ELHIGASEIN LYAKAVMDRI LKDHQIVVDI PHGEAWLRDD EERPMILIAG GTGFSYARSI LLTALARNPN RDITIYWGGR EEQHLYDLCE LEALSLKHPG LQVVPVVEQP EAGWRGRTGT VLTAVLQDHG TLAEHDIYIA GRFEMAKIAR DLFCSERNAR EDRLFGDAFA FI
Host
E Coli
Form/Format
Liquid containing glycerol
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Molecular Weight
30.1 kDa
Sequence Positions
2-233aa; Full Length of Mature Protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P0AEN1
Production Note
Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to support@mybiosource.com for more details.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

SDS-PAGE of fre recombinant protein

Escherichia Coli fre Recombinant Protein – Cat# MBS1265545

Product References and Citations for fre recombinant protein

Characterization of the flavin reductase gene (fre) of Escherichia coli and construction of a plasmid for overproduction of the enzyme.Spyrou G., Haggaard-Ljungquist E., Krook M., Joernvall H., Nilsson E., Reichard P.J. Bacteriol. 173:3673-3679(1991) FadI identification, characterization, and nucleotide sequence of a gene required for full activation of structural genes of the AD regulon in Escherichia coli.Dirusso C.C., Shea O. Saviranta P.J. Analysis of the Escherichia coli genome DNA sequence of the region from 84.5 to 86.5 minutes.Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.Science 257:771-778(1992) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) The haemoglobin-like protein (HMP) of Escherichia coli has ferrisiderophore reductase activity and its C-terminal domain shares homology with ferredoxin NADP+ reductases.Andrews S.C., Shipley D., Keen J.N., Findlay J.B.C., Harrison P.M., Guest J.R.FEBS Lett. 302:247-252(1992) Crystal structure of NAD(P) H:flavin oxidoreductase from Escherichia coli.Ingelman M., Ramaswamy S., Niviere V., Fontecave M., Eklund H.Biochemistry 38:7040-7049(1999)

Other Notes

Small volumes of fre recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for fre purchase

MBS1265545 is a recombinant protein targeting fre. Also known as Recombinant Escherichia coli NAD(P)H-flavin reductase (fre). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for fre. It may not necessarily be applicable to this product.

NCBI GI #
16131690
NCBI GeneID
948325
NCBI Accession #
NP_418286.1
UniProt Primary Accession #
P0AEN1
UniProt Secondary Accession #
P23486; P76768; Q2M8E7
GenBank Nucleotide #
NC_000913.3
NCBI Official Full Name
NAD(P)H-flavin reductase
NCBI Official Symbol
fre
NCBI Official Synonym Symbols
ECK3836; fadI; flrD; fsrC; hsm-6; JW3820
UniProt Protein Name
NAD(P)H-flavin reductase
UniProt Synonym Protein Names
Aquacobalamin reductase; FMN reductase; Ferrisiderophore reductase C; NAD(P)H:flavin oxidoreductase; Riboflavin reductase [NAD(P)H]
Protein Family
NAD(P)H-flavin reductase
UniProt Gene Name
fre
UniProt Synonym Gene Names
fadI; flrD; fsrC; ubiB
UniProt Entry Name
FRE_ECOLI
NCBI Protein Information
NAD(P)H-flavin reductase

NCBI Summary for fre

The fre gene was mistakenly thought to be ubiB. [More information is available at EcoGene: EG10334].

UniProt Comments for fre

Catalyzes the reduction of soluble flavins by reduced pyridine nucleotides. Seems to reduce the complexed Fe3+ iron of siderophores to Fe2+, thus releasing it from the chelator.

Research Articles on fre

1. N-terminus verified by Edman degradation on mature peptide

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with fre recombinant protein

 Reference Product  PubMed Publications
 pyrB recombinant protein  >1 publications with fre and pyrB
 aphA recombinant protein  >1 publications with fre and aphA

Pathways associated with fre recombinant protein

 Products by Pathway  Pathway Diagram
 Porphyrin And Chlorophyll Metabolism Pathway antibodies  Porphyrin And Chlorophyll Metabolism Pathway Diagram
 Porphyrin And Chlorophyll Metabolism Pathway antibodies  Porphyrin And Chlorophyll Metabolism Pathway Diagram
 Riboflavin Metabolism Pathway antibodies  Riboflavin Metabolism Pathway Diagram
 Riboflavin Metabolism Pathway antibodies  Riboflavin Metabolism Pathway Diagram
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