Soluble Calcium-Activated Nucleotidase 1 (SHAPY) Human Recombinant Protein

Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP. Involved in proteoglycan synthesis.

Cloning, expression, and characterization of a soluble calcium-activated nucleotidase, a human enzyme belonging to a new family of extracellular nucleotidases.Smith T., Hicks-Berger C., Kim S., Kirley T.Arch. Biochem. Biophys. 406:105-115(2002) Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways.Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.Oncogene 22:3307-3318(2003) Complete sequencing and characterization of 21,243 full-length human cDNAs.Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.Nat. Genet. 36:40-45(2004) Cloning, expression, and functional characterization of a Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase.Failer B.U., Braun N., Zimmermann H.J. Biol. Chem. 277:36978-36986(2002) Site-directed mutagenesis of human soluble calcium-activated nucleotidase 1 (hSCAN-1) identification of residues essential for enzyme activity and the Ca(2+) -induced conformational change.Yang M., Kirley T.L.Biochemistry 43:9185-9194(2004) Mutation of CANT1 causes Desbuquois dysplasia.Faden M., Al-Zahrani F., Arafah D., Alkuraya F.S.Am. J. Med. Genet. A 152:1157-1160(2010) Further delineation of CANT1 phenotypic spectrum and demonstration of its role in proteoglycan synthesis.Nizon M., Huber C., De Leonardis F., Merrina R., Forlino A., Fradin M., Tuysuz B., Abu-Libdeh B.Y., Alanay Y., Albrecht B., Al-Gazali L., Basaran S.Y., Clayton-Smith J., Desir J., Gill H., Greally M.T., Koparir E., van Maarle M.C., Mackay S., Mortier G., Morton J., Sillence D., Vilain C., Young I., Zerres K., Le Merrer M., Munnich A., Le Goff C., Rossi A., Cormier-Daire V.Hum. Mutat. 33:1261-1266(2012) Structure and protein design of a human platelet function inhibitor.Dai J., Liu J., Deng Y., Smith T.M., Lu M.Cell 116:649-659(2004) Identification of CANT1 mutations in Desbuquois dysplasia.Huber C., Oules B., Bertoli M., Chami M., Fradin M., Alanay Y., Al-Gazali L.I., Ausems M.G., Bitoun P., Cavalcanti D.P., Krebs A., Le Merrer M., Mortier G., Shafeghati Y., Superti-Furga A., Robertson S.P., Le Goff C., Muda A.O., Paterlini-Brechot P., Munnich A., Cormier-Daire V.Am. J. Hum. Genet. 85:706-710(2009) Desbuquois dysplasia type I and fetal hydrops due to novel mutations in the CANT1 gene.Laccone F., Schoner K., Krabichler B., Kluge B., Schwerdtfeger R., Schulze B., Zschocke J., Rehder H.Eur. J. Hum. Genet. 19:1133-1137(2011) A founder mutation of CANT1 common in Korean and Japanese Desbuquois dysplasia.Dai J., Kim O.H., Cho T.J., Miyake N., Song H.R., Karasugi T., Sakazume S., Ikema M., Matsui Y., Nagai T., Matsumoto N., Ohashi H., Kamatani N., Nishimura G., Furuichi T., Takahashi A., Ikegawa S.J. Hum. Genet. 56:398-400(2011) CANT1 mutation is also responsible for Desbuquois dysplasia, type 2 and Kim variant.Furuichi T., Dai J., Cho T.J., Sakazume S., Ikema M., Matsui Y., Baynam G., Nagai T., Miyake N., Matsumoto N., Ohashi H., Unger S., Superti-Furga A., Kim O.H., Nishimura G., Ikegawa S.J. Med. Genet. 48:32-37(2011)

Small volumes of SHAPY recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1265608 is a recombinant protein targeting SHAPY. Also known as Recombinant Human Soluble calcium-activated nucleotidase 1. Host: E Coli.