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NET1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS126728
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This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NET1, Polyclonal Antibody
Full Product Name
NET1 Polyclonal Antibody
Product Gene Name
anti-NET1 antibody
Product Synonym Names
NET1; ARHGEF8; NET1A
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
596
OMIM
606450
3D Structure
ModBase 3D Structure for Q7Z628
Species
Human
Immunogen
Recombinant protein of human NET1
Calculated Molecular Weight
68kDa

Product Categories/Family for anti-NET1 antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-NET1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-NET1 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200

Western Blot (WB) of anti-NET1 antibody

Western blot analysis of extracts of various cell lines, using NET1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Rabbit Anti-NET1 Polyclonal Antibody – Cat# MBS126728

Immunofluorescence (IF) of anti-NET1 antibody

Immunofluorescence analysis of HeLa cells using NET1 antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-NET1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS126728

Other Notes

Small volumes of anti-NET1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NET1 purchase

MBS126728 is an antibody targeting NET1. Also known as NET1 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NET1. It may not necessarily be applicable to this product.

NCBI GI #
52782735
NCBI GeneID
10276
NCBI Accession #
Q7Z628.1
UniProt Primary Accession #
Q7Z628
UniProt Secondary Accession #
Q12773; Q96D82; Q99903; Q9UEN6
UniProt Related Accession #
Q7Z628
NCBI Official Full Name
Neuroepithelial cell-transforming gene 1 protein
NCBI Official Synonym Full Names
neuroepithelial cell transforming 1
NCBI Official Symbol
NET1
NCBI Official Synonym Symbols
NET1A; ARHGEF8
UniProt Protein Name
Neuroepithelial cell-transforming gene 1 protein
UniProt Synonym Protein Names
Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8
Protein Family
Neuroepithelial cell-transforming gene 1 protein
UniProt Gene Name
NET1
UniProt Synonym Gene Names
ARHGEF8
UniProt Entry Name
ARHG8_HUMAN
NCBI Protein Information
neuroepithelial cell-transforming gene 1 protein; p65 Net1 proto-oncogene protein; small GTP-binding protein regulator; neuroepithelioma transforming gene 1; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene)

UniProt Comments for NET1

NET1: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; GEFs, Rac/Rho; GEFs; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: plasma membrane; nucleus; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; GTP-Rho binding

Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; myoblast migration; regulation of cell growth; signal transduction
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Research Articles on NET1

1. NET1 expression is elevated in OAC and its pre-malignant phenotype, Barrett's oesophagus. NET1 promotes OAC cell invasion and proliferation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NET1 antibody

 Reference Product  PubMed Publications
 RHOA antibody  >24 publications with NET1 and RHOA
 DLG1 antibody  >2 publications with NET1 and DLG1
 MAGI1 antibody  >2 publications with NET1 and MAGI1

Pathways associated with anti-NET1 antibody

 Products by Pathway  Pathway Diagram
 Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies  Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
 G Alpha (12/13) Signalling Events Pathway antibodies  G Alpha (12/13) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 NRAGE Signals Death Through JNK Pathway antibodies  NRAGE Signals Death Through JNK Pathway Diagram
 Regulation Of RhoA Activity Pathway antibodies  Regulation Of RhoA Activity Pathway Diagram
 Rho GTPase Cycle Pathway antibodies  Rho GTPase Cycle Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
 Signaling By Rho GTPases Pathway antibodies  Signaling By Rho GTPases Pathway Diagram
 Signalling By NGF Pathway antibodies  Signalling By NGF Pathway Diagram

Diseases associated with anti-NET1 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >14 publications with NET1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >12 publications with NET1 and Breast Neoplasms
 Liver Diseases Antibodies  >9 publications with NET1 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >8 publications with NET1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >8 publications with NET1 and Liver Neoplasms
 Brain Diseases Antibodies  >4 publications with NET1 and Brain Diseases
 Neoplasms, Experimental Antibodies  >3 publications with NET1 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >2 publications with NET1 and Cell Transformation, Neoplastic
 Disease Models, Animal Antibodies  >2 publications with NET1 and Disease Models, Animal
 Hypertension Antibodies  >1 publications with NET1 and Hypertension

Organs/Tissues associated with anti-NET1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >10 publications with NET1 and Brain
 Liver Antibodies  >9 publications with NET1 and Liver
 Blood Antibodies  >7 publications with NET1 and Blood
 Nerve Antibodies  >5 publications with NET1 and Nerve
 Stomach Antibodies  >4 publications with NET1 and Stomach
 Embryonic Tissue Antibodies  >4 publications with NET1 and Embryonic Tissue
 Vascular Antibodies  >3 publications with NET1 and Vascular
 Esophagus Antibodies  >2 publications with NET1 and Esophagus
 Eye Antibodies  >2 publications with NET1 and Eye
 Muscle Antibodies  >2 publications with NET1 and Muscle
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