Rabbit Anti-SMPD2 Polyclonal Antibody – Cat# MBS126786

Western blot analysis of extracts of various cell lines, using SMPD2 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

SMPD2 Antibody – Rabbit Polyclonal

Name: SMPD2 Antibody; Polyclonal; Rabbit; Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS126786
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS126786

MBS126786 is an antibody targeting SMPD2. Also known as SMPD2 Polyclonal Antibody. Reactive in Mouse, Rat. Host: Rabbit. More details

$225 / 0.02 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SMPD2, Polyclonal Antibody

Full Product Name

SMPD2 Polyclonal Antibody

Product Gene Name

anti-SMPD2 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Mouse, Rat

Purity/Purification

Affinity Purification

Molecular Weight

423

OMIM

603498

3D Structure

ModBase 3D Structure for O60906

Species

Human

Immunogen

Recombinant Protein

Product Categories/Family for anti-SMPD2 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-SMPD2 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Other Notes

Small volumes of anti-SMPD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SMPD2. It may not necessarily be applicable to this product.

NCBI GI #

160332206

NCBI GeneID

6610

NCBI Accession #

O60906.2

UniProt Primary Accession #

O60906

UniProt Secondary Accession #

Q5TED1; Q9BWR3

UniProt Related Accession #

O60906

NCBI Official Full Name

Sphingomyelin phosphodiesterase 2

NCBI Official Synonym Full Names

sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)

NCBI Official Symbol

SMPD2

NCBI Official Synonym Symbols

ISC1; NSMASE; NSMASE1

UniProt Protein Name

Sphingomyelin phosphodiesterase 2

UniProt Synonym Protein Names

Lyso-platelet-activating factor-phospholipase C; Lyso-PAF-PLC; Neutral sphingomyelinase; N-SMase; nSMase

UniProt Gene Name

SMPD2

UniProt Synonym Gene Names

Lyso-PAF-PLC; N-SMase; nSMase

UniProt Entry Name

NSMA_HUMAN

NCBI Protein Information

sphingomyelin phosphodiesterase 2; N-SMase; lyso-PAF-PLC; neutral sphingomyelinase; lyso-platelet-activating factor-phospholipase C

NCBI Summary for SMPD2

This gene encodes a protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. [provided by RefSeq, Oct 2009]

UniProt Comments for SMPD2

SMPD2: Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2- lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso- sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF. Belongs to the neutral sphingomyelinase family.

Protein type: Membrane protein, integral; Phosphodiesterase; Lipid Metabolism - sphingolipid; Membrane protein, multi-pass; EC 3.1.4.12

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: integral to plasma membrane; plasma membrane; caveola

Molecular Function: sphingomyelin phosphodiesterase activity; metal ion binding

Biological Process: sphingolipid metabolic process; sphingomyelin metabolic process; nerve growth factor receptor signaling pathway; response to mechanical stimulus; ceramide biosynthetic process; glycosphingolipid metabolic process

Research Articles on SMPD2

1. These data suggest that Ox-LDL activates N-SMase-ceramide signaling pathway, and stimulates phosphorylation of p38 MAPK, leading to apoptosis in vascular smooth muscle cells, which initiates VSMC calcification.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMPD2 antibody

Reference Product	PubMed Publications
SMPD1 antibody	>3 publications with SMPD2 and SMPD1
SMPD3 antibody	>1 publications with SMPD2 and SMPD3

Pathways associated with anti-SMPD2 antibody

Products by Pathway	Pathway Diagram
Ceramide Signalling Pathway antibodies	Ceramide Signalling Pathway Diagram
Glycosphingolipid Metabolism Pathway antibodies	Glycosphingolipid Metabolism Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signalling By NGF Pathway antibodies	Signalling By NGF Pathway Diagram
Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram
Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram
Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram

Diseases associated with anti-SMPD2 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>1 publications with SMPD2 and Inflammation

Organs/Tissues associated with anti-SMPD2 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2 publications with SMPD2 and Brain
Embryonic Tissue Antibodies	>1 publications with SMPD2 and Embryonic Tissue