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SHMT2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS126805
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This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SHMT2, Polyclonal Antibody
Full Product Name
SHMT2 Polyclonal Antibody
Product Gene Name
anti-SHMT2 antibody
Product Synonym Names
SHMT2; GLYA; SHMT
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
504
OMIM
138450
3D Structure
ModBase 3D Structure for P34897
Species
Human
Immunogen
Recombinant protein of human SHMT2
Calculated Molecular Weight
55kDa

Product Categories/Family for anti-SHMT2 antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-SHMT2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-SHMT2 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200

Western Blot (WB) of anti-SHMT2 antibody

Western blot analysis of extracts of various cell lines, using SHMT2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Rabbit Anti-SHMT2 Polyclonal Antibody – Cat# MBS126805

Immunohistochemistry (IHC) of anti-SHMT2 antibody

Immunohistochemistry of paraffin-embedded rat kidney using SHMT2 Antibody at dilution of 1:100 (40x lens).
Rabbit Anti-SHMT2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126805

Immunohistochemistry (IHC) of anti-SHMT2 antibody

Immunohistochemistry of paraffin-embedded human lung cancer using SHMT2 Antibody at dilution of 1:100 (40x lens).
Rabbit Anti-SHMT2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126805

Immunohistochemistry (IHC) of anti-SHMT2 antibody

Immunohistochemistry of paraffin-embedded human liver cancer using SHMT2 Antibody at dilution of 1:100 (40x lens).
Rabbit Anti-SHMT2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS126805

Immunofluorescence (IF) of anti-SHMT2 antibody

Immunofluorescence analysis of HepG2 cells using SHMT2 antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-SHMT2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS126805

Immunofluorescence (IF) of anti-SHMT2 antibody

Immunofluorescence analysis of HeLa cells using SHMT2 antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-SHMT2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS126805

Other Notes

Small volumes of anti-SHMT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SHMT2 purchase

MBS126805 is an antibody targeting SHMT2. Also known as SHMT2 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SHMT2. It may not necessarily be applicable to this product.

NCBI GI #
6226865
NCBI GeneID
6472
NCBI Accession #
P34897.3
UniProt Primary Accession #
P34897
UniProt Secondary Accession #
O00740; Q8N1A5; B7Z9F1; E7EQ19; E7EU43
UniProt Related Accession #
P34897
NCBI Official Full Name
Serine hydroxymethyltransferase, mitochondrial
NCBI Official Synonym Full Names
serine hydroxymethyltransferase 2 (mitochondrial)
NCBI Official Symbol
SHMT2
NCBI Official Synonym Symbols
GLYA; SHMT; HEL-S-51e
UniProt Protein Name
Serine hydroxymethyltransferase, mitochondrial
UniProt Synonym Protein Names
Glycine hydroxymethyltransferase; Serine methylase
UniProt Gene Name
SHMT2
UniProt Synonym Gene Names
SHMT
UniProt Entry Name
GLYM_HUMAN
NCBI Protein Information
serine hydroxymethyltransferase, mitochondrial; GLY A+; serine aldolase; serine methylase; threonine aldolase; serine hydroxymethylase; glycine hydroxymethyltransferase; epididymis secretory sperm binding protein Li 51e; glycine auxotroph A, human complement for hamster

UniProt Comments for SHMT2

SHMT2: Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA. Belongs to the SHMT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase; Energy Metabolism - methane; Cofactor and Vitamin Metabolism - one carbon pool by folate; Mitochondrial; EC 2.1.2.1; Other Amino Acids Metabolism - cyanoamino acid

Chromosomal Location of Human Ortholog: 12q12-q14

Cellular Component: microtubule cytoskeleton; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; mitochondrial intermembrane space

Molecular Function: L-allo-threonine aldolase activity; identical protein binding; amino acid binding; glycine hydroxymethyltransferase activity; chromatin binding; pyridoxal phosphate binding

Biological Process: L-serine biosynthetic process; positive regulation of cell proliferation; one-carbon compound metabolic process; glycine biosynthetic process from serine; protein homotetramerization
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Research Articles on SHMT2

1. The present study not only describes individual genetic variation that directly affects SHMT1 and SHMT2 activity, but provided insight into the overall regulation of the Folate and Methionine Cycles.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SHMT2 antibody

 Reference Product  PubMed Publications
 TYMS antibody  >2 publications with SHMT2 and TYMS
 MTHFR antibody  >1 publications with SHMT2 and MTHFR
 ATIC antibody  >1 publications with SHMT2 and ATIC

Pathways associated with anti-SHMT2 antibody

 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 C1-unit Interconversion, Eukaryotes Pathway antibodies  C1-unit Interconversion, Eukaryotes Pathway Diagram
 C1-unit Interconversion, Eukaryotes Pathway antibodies  C1-unit Interconversion, Eukaryotes Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Cyanoamino Acid Metabolism Pathway antibodies  Cyanoamino Acid Metabolism Pathway Diagram
 Cyanoamino Acid Metabolism Pathway antibodies  Cyanoamino Acid Metabolism Pathway Diagram
 Glycine Metabolism Pathway antibodies  Glycine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram

Diseases associated with anti-SHMT2 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1 publications with SHMT2 and Nervous System Diseases
 Brain Diseases Antibodies  >1 publications with SHMT2 and Brain Diseases

Organs/Tissues associated with anti-SHMT2 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >3 publications with SHMT2 and Lung
 Blood Antibodies  >2 publications with SHMT2 and Blood
 Liver Antibodies  >2 publications with SHMT2 and Liver
 Brain Antibodies  >2 publications with SHMT2 and Brain
 Adipose Tissue Antibodies  >1 publications with SHMT2 and Adipose Tissue
 Vascular Antibodies  >1 publications with SHMT2 and Vascular
 Ovary Antibodies  >1 publications with SHMT2 and Ovary
 Connective Tissue Antibodies  >1 publications with SHMT2 and Connective Tissue
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