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POLB Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS127000
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The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage.… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
POLB, Polyclonal Antibody
Full Product Name
POLB Polyclonal Antibody
Product Gene Name
anti-POLB antibody
Product Synonym Names
MGC125976; POLB
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Molecular Weight
Calculated MW: 38kDa
Observed: 37kDa
OMIM
174760
3D Structure
ModBase 3D Structure for P06746
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human POLB (NP_002681.1).

Product Categories/Family for anti-POLB antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-POLB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-POLB antibody
WB: 1:200 - 1:1000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200

Western Blot (WB) of anti-POLB antibody

Western blot analysis of extracts of various cell lines, using POLB antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Rabbit Anti-POLB Polyclonal Antibody – Cat# MBS127000

Western Blot (WB) of anti-POLB antibody

Western blot analysis of extracts fromnormal (control) and POLB knockout(KO) HeLa cells, using POLB antibody(MBS127000) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ( MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit. Exposure time: 30s
Rabbit Anti-POLB Polyclonal Antibody, Western Blot (WB) – Cat# MBS127000

Immunofluorescence (IF) of anti-POLB antibody

Immunofluorescence analysis of U2OS cells using POLB antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-POLB Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS127000

Immunohistochemistry (IHC) of anti-POLB antibody

Immunohistochemistry of paraffin-embedded Human stomach using POLB antibody (MBS127000) at dilution of1:100 (40x lens).
Rabbit Anti-POLB Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS127000

Immunohistochemistry (IHC) of anti-POLB antibody

Immunohistochemistry of paraffin-embedded Mouse kidney using POLB antibody (MBS127000) at dilution of 1:100 (40x lens).
Rabbit Anti-POLB Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS127000

Other Notes

Small volumes of anti-POLB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLB purchase

MBS127000 is an antibody targeting POLB. Also known as POLB Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for POLB. It may not necessarily be applicable to this product.

NCBI GI #
544186
NCBI GeneID
5423
NCBI Accession #
P06746.3
UniProt Primary Accession #
P06746
UniProt Secondary Accession #
Q3KP48; Q6FI34; B2RC78
UniProt Related Accession #
P06746
NCBI Official Full Name
DNA polymerase beta
NCBI Official Synonym Full Names
polymerase (DNA directed), beta
NCBI Official Symbol
POLB
UniProt Protein Name
DNA polymerase beta
Protein Family
DNA polymerase
UniProt Gene Name
POLB
UniProt Entry Name
DPOLB_HUMAN
NCBI Protein Information
DNA polymerase beta; DNA pol beta; mutant DNA polymerase beta; DNA polymerase beta subunit

UniProt Comments for POLB

POLB: Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Monomer. Interacts with APEX1, HUWE1/ARF-BP1, STUB1/CHIP and USP47. Belongs to the DNA polymerase type-X family.

Protein type: DNA replication; EC 2.7.7.7; DNA repair, damage; Lyase; Transferase

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: nucleoplasm; microtubule; spindle microtubule; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; metal ion binding; microtubule binding; damaged DNA binding; lyase activity; DNA-directed DNA polymerase activity

Biological Process: base-excision repair, gap-filling; neuron apoptosis; response to ethanol; DNA damage response, signal transduction resulting in induction of apoptosis; base-excision repair; pyrimidine dimer repair; nucleotide-excision repair, DNA gap filling; DNA-dependent DNA replication; DNA repair; response to DNA damage stimulus
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Research Articles on POLB

1. Upregulation of DNA polymerase beta is associated with chemoresistance in esophageal carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POLB antibody

 Reference Product  PubMed Publications
 XRCC1 antibody  >12 publications with POLB and XRCC1
 PCNA antibody  >10 publications with POLB and PCNA
 LIG3 antibody  >9 publications with POLB and LIG3
 APEX1 antibody  >9 publications with POLB and APEX1
 PARP1 antibody  >2 publications with POLB and PARP1
 POLD1 antibody  >2 publications with POLB and POLD1

Pathways associated with anti-POLB antibody

 Products by Pathway  Pathway Diagram
 BER Complex Pathway antibodies  BER Complex Pathway Diagram
 BER Complex Pathway antibodies  BER Complex Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway antibodies  Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Nucleotide Metabolism Pathway antibodies  Nucleotide Metabolism Pathway Diagram

Diseases associated with anti-POLB antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >10 publications with POLB and Carcinoma
 Lung Neoplasms Antibodies  >4 publications with POLB and Lung Neoplasms
 Urinary Bladder Neoplasms Antibodies  >4 publications with POLB and Urinary Bladder Neoplasms
 Cell Transformation, Neoplastic Antibodies  >3 publications with POLB and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >3 publications with POLB and Breast Neoplasms
 Pancreatic Neoplasms Antibodies  >2 publications with POLB and Pancreatic Neoplasms
 Anemia Antibodies  >2 publications with POLB and Anemia
 Hyperplasia Antibodies  >2 publications with POLB and Hyperplasia
 Uterine Cervical Neoplasms Antibodies  >2 publications with POLB and Uterine Cervical Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with POLB and Neoplasms, Experimental

Organs/Tissues associated with anti-POLB antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >6 publications with POLB and Lung
 Blood Antibodies  >4 publications with POLB and Blood
 Liver Antibodies  >3 publications with POLB and Liver
 Kidney Antibodies  >2 publications with POLB and Kidney
 Lymph Node Antibodies  >2 publications with POLB and Lymph Node
 Lymph Antibodies  >2 publications with POLB and Lymph
 Brain Antibodies  >2 publications with POLB and Brain
 Eye Antibodies  >1 publications with POLB and Eye
 Embryonic Tissue Antibodies  >1 publications with POLB and Embryonic Tissue
 Stomach Antibodies  >1 publications with POLB and Stomach
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