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COL1A1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS127111
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This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
COL1A1, Polyclonal Antibody
Full Product Name
COL1A1
Product Gene Name
anti-COL1A1 antibody
Product Synonym Names
OI1; OI2; OI3; OI4; EDSC; CAFYD; EDSARTH1; Collagen I/COL1A1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3
Purity/Purification
Affinity purification
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Molecular Weight
Observed MW: 120-130kDa/220kDa
Calculated MW: 139kDa
Chromosome Location
Chromosome: 17; NC_000017.10 (48261457..48279003, complement). Location: 17q21.33
OMIM
114000
3D Structure
ModBase 3D Structure for P02452
Cross-Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-COL1A1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immynocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-COL1A1 antibody
WB: 1:1000 - 1:6000
IHC-P: 1:100 - 1:800
IF/ICC: 1:50 - 1:200
ELISA: Recommended starting concentration is 1 ug/mL. Please optimize the concentration based on your specific assay requirements.

Western Blot (WB) of anti-COL1A1 antibody

Western blot analysis of extracts of mouse heart, using COL1A1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.
Rabbit Anti-COL1A1 Polyclonal Antibody – Cat# MBS127111

Immunohistochemistry (IHC) of anti-COL1A1 antibody

Immunohistochemistry of paraffin-embedded human esophageal cancer using COL1A1 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-COL1A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS127111

Immunohistochemistry (IHC) of anti-COL1A1 antibody

Immunohistochemistry of paraffin-embedded human prostate cancer using COL1A1 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-COL1A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS127111

Immunohistochemistry (IHC) of anti-COL1A1 antibody

Immunohistochemistry of paraffin-embedded human esophagus using COL1A1 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-COL1A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS127111

Immunofluorescence (IF) of anti-COL1A1 antibody

Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A1 Rabbit pAb (MBS127111) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Rabbit Anti-COL1A1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS127111

Immunofluorescence (IF) of anti-COL1A1 antibody

Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A1 Rabbit pAb (MBS127111) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Rabbit Anti-COL1A1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS127111

Immunofluorescence (IF) of anti-COL1A1 antibody

Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A1 Rabbit pAb (MBS127111) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Rabbit Anti-COL1A1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS127111

Western Blot (WB) of anti-COL1A1 antibody

Western blot analysis of various lysates using Collagen I/COL1A1 Rabbit pAb (MBS127111) at 1:5000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Negative control (NC): HT-29.
Exposure time: 30s.
Rabbit Anti-COL1A1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS127111

Immunohistochemistry (IHC) of anti-COL1A1 antibody

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Collagen I/COL1A1 Rabbit pAb (MBS127111) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Rabbit Anti-COL1A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS127111

Immunohistochemistry (IHC) of anti-COL1A1 antibody

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Collagen I/COL1A1 Rabbit pAb (MBS127111) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Rabbit Anti-COL1A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS127111

Immunohistochemistry (IHC) of anti-COL1A1 antibody

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Collagen I/COL1A1 Rabbit pAb (MBS127111) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Rabbit Anti-COL1A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS127111

Immunohistochemistry of anti-COL1A1 antibody

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using Collagen I/COL1A1 Rabbit pAb (MBS127111) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Rabbit Anti-COL1A1 Polyclonal Antibody, Immunohistochemistry – Cat# MBS127111

Other Notes

Small volumes of anti-COL1A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COL1A1 purchase

MBS127111 is an antibody targeting COL1A1. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for COL1A1. It may not necessarily be applicable to this product.

NCBI GI #
110349772
NCBI GeneID
1277
NCBI Accession #
NP_000079.2
UniProt Primary Accession #
P02452
UniProt Secondary Accession #
O76045; P78441; Q13896; Q13902; Q13903; Q14037; Q14992; Q15176; Q15201; Q16050; Q59F64
UniProt Related Accession #
P02452
GenBank Nucleotide #
NM_000088.3
NCBI Official Full Name
collagen alpha-1(I) chain preproprotein
NCBI Official Synonym Full Names
collagen, type I, alpha 1
NCBI Official Symbol
COL1A1
NCBI Official Synonym Symbols
OI4
UniProt Protein Name
Collagen alpha-1(I) chain
UniProt Synonym Protein Names
Alpha-1 type I collagen
Protein Family
Collagen
UniProt Gene Name
COL1A1
UniProt Entry Name
CO1A1_HUMAN
NCBI Protein Information
collagen alpha-1(I) chain; collagen alpha-1(I) chain; alpha-1 type I collagen; pro-alpha-1 collagen type 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone, alpha-1 chain

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-COL1A1 antibody

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Collagen Biosynthesis And Modifying Enzymes Pathway antibodies  Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram

Diseases associated with anti-COL1A1 antibody

 Disease Name  Pubmed Publications
 Osteogenesis Imperfecta Antibodies  >255 publications with COL1A1 and Osteogenesis Imperfecta
 Fibrosis Antibodies  >143 publications with COL1A1 and Fibrosis
 Neoplasms Antibodies  >142 publications with COL1A1 and Neoplasms
 Osteoporosis Antibodies  >102 publications with COL1A1 and Osteoporosis
 Cardiovascular Diseases Antibodies  >68 publications with COL1A1 and Cardiovascular Diseases
 Inflammation Antibodies  >50 publications with COL1A1 and Inflammation
 Liver Diseases Antibodies  >35 publications with COL1A1 and Liver Diseases
 Heart Diseases Antibodies  >30 publications with COL1A1 and Heart Diseases
 Necrosis Antibodies  >25 publications with COL1A1 and Necrosis
 Liver Cirrhosis Antibodies  >19 publications with COL1A1 and Liver Cirrhosis

Organs/Tissues associated with anti-COL1A1 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >494 publications with COL1A1 and Bone
 Connective Tissue Antibodies  >217 publications with COL1A1 and Connective Tissue
 Skin Antibodies  >210 publications with COL1A1 and Skin
 Muscle Antibodies  >99 publications with COL1A1 and Muscle
 Bone Marrow Antibodies  >82 publications with COL1A1 and Bone Marrow
 Lung Antibodies  >57 publications with COL1A1 and Lung
 Mouth Antibodies  >37 publications with COL1A1 and Mouth
 Heart Antibodies  >36 publications with COL1A1 and Heart
 Embryonic Tissue Antibodies  >34 publications with COL1A1 and Embryonic Tissue
 Kidney Antibodies  >31 publications with COL1A1 and Kidney
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