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U2AF2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS127135
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U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
U2AF2, Polyclonal Antibody
Full Product Name
U2AF2 Polyclonal Antibody
Product Gene Name
anti-U2AF2 antibody
Product Synonym Names
U2AF65
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
475
OMIM
191318
3D Structure
ModBase 3D Structure for P26368
Species
Human
Immunogen
Recombinant protein of human U2AF2
Calculated Molecular Weight
53kDa

Product Categories/Family for anti-U2AF2 antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-U2AF2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-U2AF2 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200

Western Blot (WB) of anti-U2AF2 antibody

Western blot analysis of extracts of various cell lines, using U2AF2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Rabbit Anti-U2AF2 Polyclonal Antibody – Cat# MBS127135

Immunofluorescence (IF) of anti-U2AF2 antibody

Immunofluorescence analysis of U2OS cells using U2AF2 antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-U2AF2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS127135

Immunoprecipitation (IP) of anti-U2AF2 antibody

Immunoprecipitation analysis of 200ug extracts of SW620 cells using 1ug U2AF2 antibody. Western blot was performed from the immunoprecipitate using U2AF2 antibody at a dilition of 1:500.
Rabbit Anti-U2AF2 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS127135

Other Notes

Small volumes of anti-U2AF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for U2AF2 purchase

MBS127135 is an antibody targeting U2AF2. Also known as U2AF2 Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for U2AF2. It may not necessarily be applicable to this product.

NCBI GI #
267188
NCBI GeneID
11338
NCBI Accession #
P26368.4
UniProt Primary Accession #
P26368
UniProt Secondary Accession #
Q96HC5
UniProt Related Accession #
P26368
NCBI Official Full Name
Splicing factor U2AF 65 kDa subunit
NCBI Official Synonym Full Names
U2 small nuclear RNA auxiliary factor 2
NCBI Official Symbol
U2AF2
NCBI Official Synonym Symbols
U2AF65
UniProt Protein Name
Splicing factor U2AF 65 kDa subunit
UniProt Synonym Protein Names
U2 auxiliary factor 65 kDa subunit; hU2AF(65); hU2AF65; U2 snRNP auxiliary factor large subunit
Protein Family
Splicing factor
UniProt Gene Name
U2AF2
UniProt Synonym Gene Names
U2AF65; hU2AF(65); hU2AF65
UniProt Entry Name
U2AF2_HUMAN
NCBI Protein Information
splicing factor U2AF 65 kDa subunit; hU2AF65; U2 auxiliary factor 65 kDa subunit; U2 snRNP auxiliary factor large subunit; U2 (RNU2) small nuclear RNA auxiliary factor 2; U2 small nuclear ribonucleoprotein auxiliary factor (65kD)

UniProt Comments for U2AF2

U2AF2: Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Belongs to the splicing factor SR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Spliceosome; RNA splicing

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: nucleoplasm; spliceosome; nuclear speck

Molecular Function: protein binding; enzyme binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription; positive regulation of RNA splicing
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Research Articles on U2AF2

1. U2AF has the capacity to directly define ~88% of functional 3' splice sites in the human genome; numerous U2AF binding events also occur in intronic locations.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-U2AF2 antibody

 Reference Product  PubMed Publications
 U2AF1 antibody  >3 publications with U2AF2 and U2AF1
 SNRPA antibody  >1 publications with U2AF2 and SNRPA
 SF1 antibody  >1 publications with U2AF2 and SF1

Pathways associated with anti-U2AF2 antibody

 Products by Pathway  Pathway Diagram
 Cleavage Of Growing Transcript In The Termination Region Pathway antibodies  Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 RNA Polymerase II Transcription Pathway antibodies  RNA Polymerase II Transcription Pathway Diagram
 RNA Polymerase II Transcription Termination Pathway antibodies  RNA Polymerase II Transcription Termination Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Transport Of Mature Transcript To Cytoplasm Pathway antibodies  Transport Of Mature Transcript To Cytoplasm Pathway Diagram
 Transport Of Mature MRNA Derived From An Intron-Containing Transcript Pathway antibodies  Transport Of Mature MRNA Derived From An Intron-Containing Transcript Pathway Diagram
 MRNA 3'-end Processing Pathway antibodies  MRNA 3'-end Processing Pathway Diagram

Diseases associated with anti-U2AF2 antibody

 Disease Name  Pubmed Publications
 Gastrointestinal Diseases Antibodies  >1 publications with U2AF2 and Gastrointestinal Diseases
 Immune System Diseases Antibodies  >1 publications with U2AF2 and Immune System Diseases
 Necrosis Antibodies  >1 publications with U2AF2 and Necrosis

Organs/Tissues associated with anti-U2AF2 antibody

 Organ/Tissue Name  Pubmed Publications
 Spleen Antibodies  >2 publications with U2AF2 and Spleen
 Brain Antibodies  >2 publications with U2AF2 and Brain
 Ovary Antibodies  >2 publications with U2AF2 and Ovary
 Kidney Antibodies  >1 publications with U2AF2 and Kidney
 Muscle Antibodies  >1 publications with U2AF2 and Muscle
 Lung Antibodies  >1 publications with U2AF2 and Lung
 Skin Antibodies  >1 publications with U2AF2 and Skin
 Lymph Antibodies  >1 publications with U2AF2 and Lymph
 Intestine Antibodies  >1 publications with U2AF2 and Intestine
 Heart Antibodies  >1 publications with U2AF2 and Heart
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