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SPAM1 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS127407
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Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was… More details

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Product Overview

Online Datasheet in PDF
Product Name
SPAM1, Polyclonal Antibody
Full Product Name
SPAM1 Polyclonal Antibody
Product Gene Name
anti-SPAM1 antibody
Product Synonym Names
HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15
Clonality
Polyclonal
Isotype
IgG
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20°C. Avoid freeze/thaw cycles
Molecular Weight
Observed MW: 64KDa
Calculated MW: 58kDa
OMIM
600930
3D Structure
ModBase 3D Structure for P38567
Source
Rabbit
Category
Primary Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2)
Species Reactivity / Cross-Reactivity
Human, Mouse, Rat

Product Categories/Family for anti-SPAM1 antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-SPAM1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-p (IHC-p), Immunocytochemistry (ICC)
Application Notes for anti-SPAM1 antibody
WB 1:500 - 1:1000
IHC-P 1:50 - 1:200
IF/ICC 1:50 - 1:200

Western Blot (WB) of anti-SPAM1 antibody

Western blot analysis of extracts of various cell lines, using SPAM1 antibody (MBS127407) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Anti-SPAM1 Polyclonal Antibody – Cat# MBS127407

Immunohistochemistry of anti-SPAM1 antibody

Immunohistochemistry of paraffin-embedded rat testis using SPAM1 Rabbit pAb (MBS127407) at dilution of 1:100 (40xlens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Anti-SPAM1 Polyclonal Antibody, Immunohistochemistry – Cat# MBS127407

Immunofluorescence (IF) of anti-SPAM1 antibody

Immunofluorescence analysis of rat testis cells using SPAM1 Rabbit pAb (MBS127407) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining
Anti-SPAM1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS127407

Immunofluorescence (IF) of anti-SPAM1 antibody

Immunofluorescence analysis of human testis cells using SPAM1 Rabbit pAb (MBS127407) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-SPAM1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS127407

Other Notes

Small volumes of anti-SPAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPAM1 purchase

MBS127407 is an antibody targeting SPAM1. Also known as SPAM1 Polyclonal Antibody.

Additional Details

NCBI/Uniprot data below describe general gene information for SPAM1. It may not necessarily be applicable to this product.

NCBI GI #
585673
NCBI GeneID
6677
NCBI Accession #
P38567.1
UniProt Primary Accession #
P38567
UniProt Secondary Accession #
Q8TC30
UniProt Related Accession #
P38567
NCBI Official Full Name
Hyaluronidase PH-20
NCBI Official Synonym Full Names
sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
NCBI Official Symbol
SPAM1
NCBI Official Synonym Symbols
HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
UniProt Protein Name
Hyaluronidase PH-20
UniProt Synonym Protein Names
Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20
Protein Family
Hyaluronidase
UniProt Gene Name
SPAM1
UniProt Synonym Gene Names
HYAL3; PH20; Hyal-PH20
UniProt Entry Name
HYALP_HUMAN
NCBI Protein Information
hyaluronidase PH-20; hyal-PH20; sperm surface protein PH-20; hyaluronoglucosaminidase PH-20; epididymis secretory sperm binding protein Li 96n

NCBI Summary for SPAM1

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
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UniProt Comments for SPAM1

SPAM1: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Belongs to the glycosyl hydrolase 56 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - glycosaminoglycan degradation; Hydrolase; EC 3.2.1.35; Membrane protein, GPI anchor; Cell surface

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: plasma membrane

Molecular Function: hyalurononglucosaminidase activity

Biological Process: sperm-egg recognition; fusion of sperm to egg plasma membrane; binding of sperm to zona pellucida; single fertilization; carbohydrate metabolic process; cell adhesion; multicellular organism reproduction
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Research Articles on SPAM1

1. results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPAM1 antibody

 Reference Product  PubMed Publications
 HAS2 antibody  >2 publications with SPAM1 and HAS2

Pathways associated with anti-SPAM1 antibody

 Products by Pathway  Pathway Diagram
 Chondroitin Sulfate Degradation Pathway antibodies  Chondroitin Sulfate Degradation Pathway Diagram
 Chondroitin Sulfate Degradation Pathway antibodies  Chondroitin Sulfate Degradation Pathway Diagram
 Dermatan Sulfate Degradation Pathway antibodies  Dermatan Sulfate Degradation Pathway Diagram
 Dermatan Sulfate Degradation Pathway antibodies  Dermatan Sulfate Degradation Pathway Diagram
 Fertilization Pathway antibodies  Fertilization Pathway Diagram
 Glycosaminoglycan Degradation Pathway antibodies  Glycosaminoglycan Degradation Pathway Diagram
 Glycosaminoglycan Degradation Pathway antibodies  Glycosaminoglycan Degradation Pathway Diagram
 Interaction With Cumulus Cells Pathway antibodies  Interaction With Cumulus Cells Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Reproduction Pathway antibodies  Reproduction Pathway Diagram

Diseases associated with anti-SPAM1 antibody

 Disease Name  Pubmed Publications
 Liver Neoplasms Antibodies  >1 publications with SPAM1 and Liver Neoplasms
 Nervous System Diseases Antibodies  >1 publications with SPAM1 and Nervous System Diseases
 Fetal Death Antibodies  >1 publications with SPAM1 and Fetal Death

Organs/Tissues associated with anti-SPAM1 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >26 publications with SPAM1 and Testis
 Brain Antibodies  >1 publications with SPAM1 and Brain
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