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HSF2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS127443
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The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HSF2, Polyclonal Antibody
Full Product Name
HSF2 Polyclonal Antibody
Product Gene Name
Product Synonym Names
HSF 2; HSTF 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
230
OMIM
140581
3D Structure
ModBase 3D Structure for Q03933
Species
Human
Immunogen
Recombinant protein of human HSF2
Calculated Molecular Weight
60kDa

Product Categories/Family for anti-HSF2 antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-HSF2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-HSF2 antibody
WB: 1:500 - 1:2000
IF: 1:50 - 1:200

Western Blot (WB) of anti-HSF2 antibody

Western blot analysis of extracts of various cell lines, using HSF2 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Rabbit Anti-HSF2 Polyclonal Antibody – Cat# MBS127443

Immunohistochemistry (IHC) of anti-HSF2 antibody

Immunohistochemistry of paraffin-embedded rat liver using HSF2 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-HSF2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS127443

Immunofluorescence (IF) of anti-HSF2 antibody

Immunofluorescence analysis of A549 cells using HSF2 antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-HSF2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS127443

Other Notes

Small volumes of anti-HSF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSF2 purchase

MBS127443 is an antibody targeting HSF2. Also known as HSF2 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HSF2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Heat shock factor protein 2
NCBI Official Synonym Full Names
heat shock transcription factor 2
NCBI Official Symbol
HSF2
NCBI Official Synonym Symbols
HSF 2; HSTF 2
UniProt Protein Name
Heat shock factor protein 2
UniProt Synonym Protein Names
Heat shock transcription factor 2; HSTF 2
UniProt Gene Name
HSF2
UniProt Synonym Gene Names
HSTF2; HSF 2; HSTF 2
UniProt Entry Name
HSF2_HUMAN
NCBI Protein Information
heat shock factor protein 2

UniProt Comments for HSF2

HSF2: DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Belongs to the HSF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6q22.31

Cellular Component: cytoplasm; nucleus

Molecular Function: protein homodimerization activity; transcription coactivator activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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