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CD33 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS127495
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CD33 is a type I transmembrane glycoprotein that is found on granulocyte and macrophage precursors in the bone marrow, but is absent from pluripotent stem cells. CD33 is also expressed on monocytes in peripheral blood. It is used as a marker to distinguish… More details

$225 / 0.02 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD33, Polyclonal Antibody
Full Product Name
CD33 Polyclonal Antibody
Product Gene Name
anti-CD33 antibody
Product Synonym Names
FLJ00391; SIGLEC-3; SIGLEC3; p67
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20 degree c. Avoid freeze / thaw cycles.
Molecular Weight
Calculated MW: 25kDa/33kDa/39kDa
Observed MW: 77kDa
OMIM
159590
3D Structure
ModBase 3D Structure for P20138
Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 49-259 of human CD33 (NP_001763.3).
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Product Categories/Family for anti-CD33 antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CD33 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-CD33 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50-1:200

Western Blot (WB) of anti-CD33 antibody

Western blot analysis of extracts ofvarious cell lines, using SIGLEC3/CD33 antibody (MBS127495) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Rabbit Anti-CD33 Polyclonal Antibody – Cat# MBS127495

Immunohistochemistry of anti-CD33 antibody

Immunohistochemistry of paraffin-embedded mouse spleen using SIGLEC3/CD33 antibody (MBS127495) atdilution of 1:200 (40x lens).
Rabbit Anti-CD33 Polyclonal Antibody, Immunohistochemistry – Cat# MBS127495

Immunofluorescence of anti-CD33 antibody

Immunofluorescence analysis of THP-1 cells using CD33 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Rabbit Anti-CD33 Polyclonal Antibody, Immunofluorescence – Cat# MBS127495

Other Notes

Small volumes of anti-CD33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD33 purchase

MBS127495 is an antibody targeting CD33. Also known as CD33 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD33. It may not necessarily be applicable to this product.

NCBI GI #
116241290
NCBI GeneID
945
NCBI Accession #
P20138.2
UniProt Primary Accession #
P20138
UniProt Secondary Accession #
Q8TD24; B4E3P8; C9JEN7; F8WAL2
UniProt Related Accession #
P20138
NCBI Official Full Name
Myeloid cell surface antigen CD33
NCBI Official Synonym Full Names
CD33 molecule
NCBI Official Symbol
CD33
NCBI Official Synonym Symbols
p67; SIGLEC3; SIGLEC-3
UniProt Protein Name
Myeloid cell surface antigen CD33
UniProt Synonym Protein Names
Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD_antigen: CD33
Protein Family
Myeloid cell surface antigen
UniProt Gene Name
CD33
UniProt Synonym Gene Names
SIGLEC3; Siglec-3
UniProt Entry Name
CD33_HUMAN
NCBI Protein Information
myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3

UniProt Comments for CD33

CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; receptor activity; carbohydrate binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; signal transduction; cell adhesion
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Research Articles on CD33

1. The rs3865444(C) risk allele is strongly associated with greater expression of CD33 exon 2 and increased Alzheimer's disease susceptibility. [Meta-analysis]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD33 antibody

 Reference Product  PubMed Publications
 CD34 antibody  >1065 publications with CD33 and CD34
 SRC antibody  >20 publications with CD33 and SRC
 PTPN11 antibody  >11 publications with CD33 and PTPN11
 PTPN6 antibody  >7 publications with CD33 and PTPN6
 CBL antibody  >3 publications with CD33 and CBL
 UBC antibody  >3 publications with CD33 and UBC
 PVR antibody  >1 publications with CD33 and PVR

Pathways associated with anti-CD33 antibody

 Products by Pathway  Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-CD33 antibody

 Disease Name  Pubmed Publications
 Leukemia, Myeloid Antibodies  >1323 publications with CD33 and Leukemia, Myeloid
 Leukemia, Myeloid, Acute Antibodies  >1160 publications with CD33 and Leukemia, Myeloid, Acute
 Necrosis Antibodies  >92 publications with CD33 and Necrosis
 Death Antibodies  >78 publications with CD33 and Death
 Cardiovascular Diseases Antibodies  >77 publications with CD33 and Cardiovascular Diseases
 Inflammation Antibodies  >67 publications with CD33 and Inflammation
 Respiratory Tract Diseases Antibodies  >44 publications with CD33 and Respiratory Tract Diseases
 Brain Diseases Antibodies  >40 publications with CD33 and Brain Diseases
 Breast Neoplasms Antibodies  >39 publications with CD33 and Breast Neoplasms
 Leukocytosis Antibodies  >32 publications with CD33 and Leukocytosis

Organs/Tissues associated with anti-CD33 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1414 publications with CD33 and Blood
 Bone Antibodies  >982 publications with CD33 and Bone
 Bone Marrow Antibodies  >971 publications with CD33 and Bone Marrow
 Liver Antibodies  >85 publications with CD33 and Liver
 Lymph Node Antibodies  >53 publications with CD33 and Lymph Node
 Lung Antibodies  >50 publications with CD33 and Lung
 Brain Antibodies  >31 publications with CD33 and Brain
 Thymus Antibodies  >29 publications with CD33 and Thymus
 Kidney Antibodies  >19 publications with CD33 and Kidney
 Prostate Antibodies  >10 publications with CD33 and Prostate
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