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GAD2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS127577
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This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GAD2, Polyclonal Antibody
Full Product Name
GAD2 Polyclonal Antibody
Product Gene Name
anti-GAD2 antibody
Product Synonym Names
GAD2; GAD65; MGC161605; MGC161607
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Molecular Weight
Observed MW: 60KDa
Calculated MW: 65kDa
OMIM
138275
3D Structure
ModBase 3D Structure for Q05329
Cross-Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 401-585 of human GAD2 (NP_001127838.1).

Product Categories/Family for anti-GAD2 antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-GAD2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-GAD2 antibody
WB: 1:500 - 1:2000
IF: 1:10 - 1:100

Western Blot (WB) of anti-GAD2 antibody

Western blot analysis of extracts of various cell lines, using GAD2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) MBS128200 ) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Rabbit Anti-GAD2 Polyclonal Antibody – Cat# MBS127577

Western Blot (WB) of anti-GAD2 antibody

Western blot analysis of extracts of various cell lines, using GAD2 antibody (MBS127577) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s
Rabbit Anti-GAD2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS127577

Immunohistochemistry (IHC) of anti-GAD2 antibody

Immunohistochemistry of paraffin-embedded rat brain using GAD2 Antibody at dilution of 1:100 (40x lens).
Rabbit Anti-GAD2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS127577

Immunofluorescence (IF) of anti-GAD2 antibody

Immunofluorescence analysis of A549 cells using GAD2 antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-GAD2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS127577

Immunofluorescence (IF) of anti-GAD2 antibody

Immunofluorescence analysis of rat brain using GAD2 Rabbit pAb (MBS127577) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Rabbit Anti-GAD2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS127577

Immunofluorescence (IF) of anti-GAD2 antibody

Immunofluorescence analysis of mouse brain using GAD2 Rabbit pAb (MBS127577) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Rabbit Anti-GAD2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS127577

Other Notes

Small volumes of anti-GAD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAD2 purchase

MBS127577 is an antibody targeting GAD2. Also known as GAD2 Polyclonal Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GAD2. It may not necessarily be applicable to this product.

NCBI GI #
1352216
NCBI GeneID
2572
NCBI Accession #
Q05329.1
UniProt Primary Accession #
Q05329
UniProt Secondary Accession #
Q9UD87
UniProt Related Accession #
Q05329
NCBI Official Full Name
Glutamate decarboxylase 2
NCBI Official Synonym Full Names
glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
NCBI Official Symbol
GAD2
NCBI Official Synonym Symbols
GAD65
UniProt Protein Name
Glutamate decarboxylase 2
UniProt Synonym Protein Names
65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
Protein Family
Glutamate decarboxylase
UniProt Gene Name
GAD2
UniProt Synonym Gene Names
GAD65; GAD-65
UniProt Entry Name
DCE2_HUMAN
NCBI Protein Information
glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform

UniProt Comments for GAD2

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Lyase; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: presynaptic membrane; Golgi membrane; synaptic vesicle membrane; axon; perinuclear region of cytoplasm; plasma membrane; cytosol; cell junction

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process
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Research Articles on GAD2

1. GAD2 SNPs significantly associate with early-onset obsessive compulsive disorder.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GAD2 antibody

 Reference Product  PubMed Publications
 GAD1 antibody  >50 publications with GAD2 and GAD1
 GLUL antibody  >2 publications with GAD2 and GLUL
 GLS antibody  >1 publications with GAD2 and GLS
 INS antibody  >1 publications with GAD2 and INS
 SLC32A1 antibody  >1 publications with GAD2 and SLC32A1
 ABAT antibody  >1 publications with GAD2 and ABAT

Pathways associated with anti-GAD2 antibody

 Products by Pathway  Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA Synthesis Pathway antibodies  GABA Synthesis Pathway Diagram
 GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies  GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram

Diseases associated with anti-GAD2 antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >20 publications with GAD2 and Mental Disorders
 Nervous System Diseases Antibodies  >13 publications with GAD2 and Nervous System Diseases
 Brain Diseases Antibodies  >11 publications with GAD2 and Brain Diseases
 Schizophrenia Antibodies  >10 publications with GAD2 and Schizophrenia
 Epilepsy Antibodies  >6 publications with GAD2 and Epilepsy
 Seizures Antibodies  >6 publications with GAD2 and Seizures
 Depressive Disorder Antibodies  >4 publications with GAD2 and Depressive Disorder
 Inflammation Antibodies  >4 publications with GAD2 and Inflammation
 Movement Disorders Antibodies  >2 publications with GAD2 and Movement Disorders
 Substance Withdrawal Syndrome Antibodies  >2 publications with GAD2 and Substance Withdrawal Syndrome

Organs/Tissues associated with anti-GAD2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >37 publications with GAD2 and Brain
 Pancreas Antibodies  >3 publications with GAD2 and Pancreas
 Uncharacterized Tissue Antibodies  >1 publications with GAD2 and Uncharacterized Tissue
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