Rabbit Anti-MYSM1 Polyclonal Antibody – Cat# MBS127723

Western blot analysis of extracts of various cell lines, using MYSM1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

MYSM1 Antibody – Rabbit Polyclonal

Name: MYSM1 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS127723
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS127723

Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes.… More details

$225 / 0.02 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MYSM1, Polyclonal Antibody

Full Product Name

MYSM1 Polyclonal Antibody

Product Gene Name

anti-MYSM1 antibody

Product Synonym Names

2ADUB; 2A-DUB; RP4-592A1.1

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

828

OMIM

612176

3D Structure

ModBase 3D Structure for Q5VVJ2

Species

Human

Route

Synthetic Peptide

Immunogen

A synthetic peptide of human MYSM1

Calculated Molecular Weight

95kD

Product Categories/Family for anti-MYSM1 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-MYSM1 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-MYSM1 antibody

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Other Notes

Small volumes of anti-MYSM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYSM1 purchase

MBS127723 is an antibody targeting MYSM1. Also known as MYSM1 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MYSM1. It may not necessarily be applicable to this product.

NCBI GI #

74756898

NCBI GeneID

114803

NCBI Accession #

Q5VVJ2.1

UniProt Primary Accession #

Q5VVJ2

UniProt Secondary Accession #

Q68DD3; Q6AI53; Q7Z3G8; Q96PX3; A8KA54; B3KX65

UniProt Related Accession #

Q5VVJ2

NCBI Official Full Name

Histone H2A deubiquitinase MYSM1

NCBI Official Synonym Full Names

Myb-like, SWIRM and MPN domains 1

NCBI Official Symbol

MYSM1

NCBI Official Synonym Symbols

2ADUB; 2A-DUB

UniProt Protein Name

Histone H2A deubiquitinase MYSM1

UniProt Synonym Protein Names

Myb-like, SWIRM and MPN domain-containing protein 1

Protein Family

Histone H2A deubiquitinase

UniProt Gene Name

MYSM1

UniProt Synonym Gene Names

KIAA1915; 2A-DUB

UniProt Entry Name

MYSM1_HUMAN

NCBI Protein Information

histone H2A deubiquitinase MYSM1; myb-like, SWIRM and MPN domain-containing protein 1

UniProt Comments for MYSM1

MYSM1: Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. Belongs to the peptidase M67A family. MYSM1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Protease; Ubiquitin-specific protease; EC 3.4.19.-

Chromosomal Location of Human Ortholog: 1p32.1

Cellular Component: nucleoplasm; microtubule cytoskeleton; nucleus

Molecular Function: DNA binding; histone binding; metallopeptidase activity; metal ion binding; ubiquitin-specific protease activity; transcription coactivator activity; protein complex binding; chromatin binding

Biological Process: chromatin remodeling; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; proteolysis

Research Articles on MYSM1

1. the biallelic truncation of MYSM1 likely represents the cause of an inherited bone marrow failure syndrome in two siblings

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MYSM1 antibody

Reference Product	PubMed Publications
EBF1 antibody	>1 publications with MYSM1 and EBF1

Organs/Tissues associated with anti-MYSM1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>7 publications with MYSM1 and Blood
Eye Antibodies	>1 publications with MYSM1 and Eye
Bone Marrow Antibodies	>1 publications with MYSM1 and Bone Marrow
Prostate Antibodies	>1 publications with MYSM1 and Prostate