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GADD45A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS127973
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This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GADD45A, Polyclonal Antibody
Full Product Name
GADD45A Polyclonal Antibody
Product Gene Name
anti-GADD45A antibody
Product Synonym Names
DDIT1; GADD45
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
165
OMIM
126335
3D Structure
ModBase 3D Structure for P24522
Species
Human
Immunogen
Recombinant protein of human GADD45A
Calculated Molecular Weight
18kDa

Product Categories/Family for anti-GADD45A antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-GADD45A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-GADD45A antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Western Blot (WB) of anti-GADD45A antibody

Western blot analysis of extracts of mouse liver, using GADD45A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Rabbit Anti-GADD45A Polyclonal Antibody – Cat# MBS127973

Other Notes

Small volumes of anti-GADD45A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GADD45A purchase

MBS127973 is an antibody targeting GADD45A. Also known as GADD45A Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GADD45A. It may not necessarily be applicable to this product.

NCBI GI #
120751
NCBI GeneID
1647
NCBI Accession #
P24522.1
UniProt Primary Accession #
P24522
UniProt Secondary Accession #
Q5TCA7; Q5TCA8
UniProt Related Accession #
P24522
NCBI Official Full Name
Growth arrest and DNA damage-inducible protein GADD45 alpha
NCBI Official Synonym Full Names
growth arrest and DNA-damage-inducible, alpha
NCBI Official Symbol
GADD45A
NCBI Official Synonym Symbols
DDIT1; GADD45
UniProt Protein Name
Growth arrest and DNA damage-inducible protein GADD45 alpha
UniProt Synonym Protein Names
DNA damage-inducible transcript 1 protein; DDIT-1
Protein Family
Growth arrest and DNA damage-inducible protein
UniProt Gene Name
GADD45A
UniProt Synonym Gene Names
DDIT1; GADD45; DDIT-1
UniProt Entry Name
GA45A_HUMAN
NCBI Protein Information
growth arrest and DNA damage-inducible protein GADD45 alpha; DDIT-1; DNA damage-inducible transcript-1; DNA-damage-inducible transcript 1; DNA damage-inducible transcript 1 protein; growth arrest and DNA-damage-inducible 45 alpha

UniProt Comments for GADD45A

GADD45A: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Interacts with MAPK14. Interacts with GADD45GIP1. Interacts with PCNA. By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediate by some kinase(s) other than PKC. Belongs to the GADD45 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Nuclear receptor co-regulator; DNA repair, damage; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p31.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of apoptosis; apoptosis; activation of MAPKKK activity; DNA damage response, signal transduction; centrosome cycle; negative regulation of protein kinase activity; positive regulation of JNK cascade; regulation of cyclin-dependent protein kinase activity; DNA repair; cell cycle arrest
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Research Articles on GADD45A

1. DNA damage-inducible transcript 1 protein(Gadd45alpha) may be involved in human trophoblast migration and invasion and may function as an important negative regulator at the foetal-maternal interface during early pregnancy

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GADD45A antibody

 Reference Product  PubMed Publications
 CDKN1A antibody  >100 publications with GADD45A and CDKN1A
 GADD45B antibody  >53 publications with GADD45A and GADD45B
 GADD45G antibody  >33 publications with GADD45A and GADD45G
 PCNA antibody  >32 publications with GADD45A and PCNA
 TP53 antibody  >29 publications with GADD45A and TP53
 CCNB1 antibody  >10 publications with GADD45A and CCNB1
 CDK1 antibody  >6 publications with GADD45A and CDK1
 MAP2K6 antibody  >3 publications with GADD45A and MAP2K6
 FOXO4 antibody  >2 publications with GADD45A and FOXO4
 MAP3K4 antibody  >2 publications with GADD45A and MAP3K4

Pathways associated with anti-GADD45A antibody

 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 ATM Signaling Pathway antibodies  ATM Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Aurora A Signaling Pathway antibodies  Aurora A Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 DNA Damage Response Pathway antibodies  DNA Damage Response Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 FoxO Family Signaling Pathway antibodies  FoxO Family Signaling Pathway Diagram

Diseases associated with anti-GADD45A antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >30 publications with GADD45A and Inflammation
 Breast Neoplasms Antibodies  >28 publications with GADD45A and Breast Neoplasms
 Adenocarcinoma Antibodies  >27 publications with GADD45A and Adenocarcinoma
 Necrosis Antibodies  >23 publications with GADD45A and Necrosis
 Prostatic Neoplasms Antibodies  >23 publications with GADD45A and Prostatic Neoplasms
 Nervous System Diseases Antibodies  >22 publications with GADD45A and Nervous System Diseases
 Liver Diseases Antibodies  >21 publications with GADD45A and Liver Diseases
 Neoplasms, Experimental Antibodies  >20 publications with GADD45A and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >18 publications with GADD45A and Lung Neoplasms
 Liver Neoplasms Antibodies  >16 publications with GADD45A and Liver Neoplasms

Organs/Tissues associated with anti-GADD45A antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >69 publications with GADD45A and Blood
 Lung Antibodies  >38 publications with GADD45A and Lung
 Liver Antibodies  >38 publications with GADD45A and Liver
 Prostate Antibodies  >28 publications with GADD45A and Prostate
 Bone Antibodies  >27 publications with GADD45A and Bone
 Vascular Antibodies  >25 publications with GADD45A and Vascular
 Skin Antibodies  >23 publications with GADD45A and Skin
 Brain Antibodies  >21 publications with GADD45A and Brain
 Muscle Antibodies  >19 publications with GADD45A and Muscle
 Embryonic Tissue Antibodies  >14 publications with GADD45A and Embryonic Tissue
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