Rabbit Anti-ENPP7 Polyclonal Antibody – Cat# MBS127989

Western blot analysis of extracts of HepG2 cells, using ENPP7 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

ENPP7 Antibody – Rabbit Polyclonal

Name: ENPP7 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS127989
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS127989

Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 (E-NPP 7) also known as alkaline sphingomyelin phosphodiesterase (Alk-SMase) or intestinal alkaline sphingomyelinase is an enzyme that in humans is encoded by the ENPP7 gene. Converts… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

ENPP7, Polyclonal Antibody

Full Product Name

ENPP7 Polyclonal Antibody

Product Gene Name

anti-ENPP7 antibody

Product Synonym Names

ALK-SMase

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

458

3D Structure

ModBase 3D Structure for Q6UWV6

Species

Human

Route

Synthetic Peptide

Immunogen

A synthetic peptide of human ENPP7

Calculated Molecular Weight

52kDa

Product Categories/Family for anti-ENPP7 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-ENPP7 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-ENPP7 antibody

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Other Notes

Small volumes of anti-ENPP7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENPP7 purchase

MBS127989 is an antibody targeting ENPP7. Also known as ENPP7 Polyclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ENPP7. It may not necessarily be applicable to this product.

NCBI GI #

134047772

NCBI GeneID

339221

NCBI Accession #

Q6UWV6.3

UniProt Primary Accession #

Q6UWV6

UniProt Secondary Accession #

Q6ZTS5; Q8IUS8

UniProt Related Accession #

Q6UWV6

NCBI Official Full Name

Ectonucleotide pyrophosphatase/phosphodiesterase family member 7

NCBI Official Synonym Full Names

ectonucleotide pyrophosphatase/phosphodiesterase 7

NCBI Official Symbol

ENPP7

NCBI Official Synonym Symbols

NPP7; NPP-7; E-NPP 7; ALK-SMase

UniProt Protein Name

Ectonucleotide pyrophosphatase/phosphodiesterase family member 7

UniProt Synonym Protein Names

Alkaline sphingomyelin phosphodiesterase; Intestinal alkaline sphingomyelinase; Alk-SMase

Protein Family

Ectonucleotide pyrophosphatase/phosphodiesterase family

UniProt Gene Name

ENPP7

UniProt Synonym Gene Names

E-NPP 7; NPP-7; Alk-SMase

UniProt Entry Name

ENPP7_HUMAN

NCBI Protein Information

ectonucleotide pyrophosphatase/phosphodiesterase family member 7; ENPP7; alkaline sphingomyelinase; intestinal alkaline sphingomyelinase; alkaline sphingomyelin phosphodiesterase

UniProt Comments for ENPP7

ENPP7: Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Protein type: Phosphodiesterase; Lipid Metabolism - sphingolipid; Cell cycle regulation; DNA replication; Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.1.4.12

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: Golgi apparatus; microvillus; membrane; integral to membrane; plasma membrane

Molecular Function: sphingomyelin phosphodiesterase activity

Biological Process: negative regulation of cell proliferation; sphingomyelin metabolic process; sphingolipid metabolic process; negative regulation of DNA replication; glycosphingolipid metabolic process; sphingomyelin catabolic process

Research Articles on ENPP7

1. The F275A mutation of NPP7 showed impaired catalytic function whereas L107F mutation showed enhanced catalytic activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ENPP7 antibody

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Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram
Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram
Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram
Sphingomyelin Metabolism/ceramide Salvage Pathway antibodies	Sphingomyelin Metabolism/ceramide Salvage Pathway Diagram