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TFAM Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS128046
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This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TFAM, Antibody
Full Product Name
TFAM Polyclonal Antibody
Product Gene Name
anti-TFAM antibody
Product Synonym Names
TFAM; MTDPS15; MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3
Isotype
IgG
Host
Rabbit
Form/Format
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Purity/Purification
Affinity purification
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Molecular Weight
Observed MW: 22kDa
Calculated MW: 25kDa/29kDa
OMIM
600438
3D Structure
ModBase 3D Structure for Q00059
Species Reactivity/
Cross-Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TFAM (NP_003192.1).

Product Categories/Family for anti-TFAM antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-TFAM antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-TFAM antibody
WB: 1:500 - 1:2000
IF: 1:50 - 1:200

Western Blot (WB) of anti-TFAM antibody

Western blot analysis of extracts of various cell lines, using TFAM antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Rabbit Anti-TFAM Antibody – Cat# MBS128046

Immunofluorescence (IF) of anti-TFAM antibody

Immunofluorescence analysis of U2OS cells using TFAM antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-TFAM Antibody, Immunofluorescence (IF) – Cat# MBS128046

Immunofluorescence (IF) of anti-TFAM antibody

Confocal immunofluorescence analysis of U2OS cells using TFAM Polyclonal Antibody (MBS128046) at dilution of 1:100. Blue: DAPI for nuclear staining.
Rabbit Anti-TFAM Antibody, Immunofluorescence (IF) – Cat# MBS128046

Other Notes

Small volumes of anti-TFAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFAM purchase

MBS128046 is an antibody targeting TFAM. Also known as TFAM Polyclonal Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TFAM. It may not necessarily be applicable to this product.

NCBI GI #
417324
NCBI GeneID
7019
NCBI Accession #
Q00059.1
UniProt Primary Accession #
Q00059
UniProt Secondary Accession #
Q5U0C6; A8MRB2; A9QXC6; B5BU05
UniProt Related Accession #
Q00059
NCBI Official Full Name
Transcription factor A, mitochondrial
NCBI Official Synonym Full Names
transcription factor A, mitochondrial
NCBI Official Symbol
TFAM
NCBI Official Synonym Symbols
TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3
UniProt Protein Name
Transcription factor A, mitochondrial
UniProt Synonym Protein Names
Mitochondrial transcription factor 1; MtTF1; Transcription factor 6; TCF-6; Transcription factor 6-like 2
Protein Family
Transcription factor
UniProt Gene Name
TFAM
UniProt Synonym Gene Names
TCF6; TCF6L2; mtTFA; MtTF1; TCF-6
UniProt Entry Name
TFAM_HUMAN
NCBI Protein Information
transcription factor A, mitochondrial; transcription factor 6; transcription factor 6-like 1; transcription factor 6-like 3; mitochondrial transcription factor 1; mitochondrial transcription factor A; transcription factor 6-like 2 (mitochondrial transcription factor)

UniProt Comments for TFAM

TFAM: regulates mitochondrial transcription. Induced in humans by caloric restriction. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Activates transcription by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Involved in cisplatin resistance. Interacts with TFB1M and TFB2M.

Protein type: Transcription regulation; DNA-binding

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: mitochondrion; mitochondrial matrix; nucleus; cytosol

Molecular Function: protein binding; heat shock protein binding; chromatin binding; transcription factor activity; DNA bending activity

Biological Process: mitochondrial respiratory chain complex assembly; chromatin remodeling; mitochondrion organization and biogenesis; regulation of transcription from RNA polymerase I promoter; positive regulation of transcription, DNA-dependent; organelle organization and biogenesis; gene expression; positive regulation of transcription from RNA polymerase II promoter; DNA-dependent DNA replication; transcription from mitochondrial promoter; transcription initiation from mitochondrial promoter
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Research Articles on TFAM

1. Data suggest that miitochondrial transcription factor A (mtTFA) may be a novel target for the treatment of pancreatic ductal adenocarcinoma (PDAC).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TFAM antibody

 Reference Product  PubMed Publications
 NRF1 antibody  >105 publications with TFAM and NRF1
 TFB2M antibody  >40 publications with TFAM and TFB2M
 TFB1M antibody  >26 publications with TFAM and TFB1M
 POLRMT antibody  >17 publications with TFAM and POLRMT
 GABPA antibody  >12 publications with TFAM and GABPA
 GABPB1 antibody  >1 publications with TFAM and GABPB1
 TXN2 antibody  >1 publications with TFAM and TXN2

Pathways associated with anti-TFAM antibody

 Products by Pathway  Pathway Diagram
 Energy Metabolism Pathway antibodies  Energy Metabolism Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Mitochondrial Gene Expression Pathway antibodies  Mitochondrial Gene Expression Pathway Diagram
 Mitochondrial Biogenesis Pathway antibodies  Mitochondrial Biogenesis Pathway Diagram
 Mitochondrial Transcription Initiation Pathway antibodies  Mitochondrial Transcription Initiation Pathway Diagram
 Organelle Biogenesis And Maintenance Pathway antibodies  Organelle Biogenesis And Maintenance Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 Transcription From Mitochondrial Promoters Pathway antibodies  Transcription From Mitochondrial Promoters Pathway Diagram

Diseases associated with anti-TFAM antibody

 Disease Name  Pubmed Publications
 Mitochondrial Diseases Antibodies  >99 publications with TFAM and Mitochondrial Diseases
 Nervous System Diseases Antibodies  >86 publications with TFAM and Nervous System Diseases
 Disease Models, Animal Antibodies  >54 publications with TFAM and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >49 publications with TFAM and Cardiovascular Diseases
 Insulin Resistance Antibodies  >41 publications with TFAM and Insulin Resistance
 Heart Diseases Antibodies  >34 publications with TFAM and Heart Diseases
 Inflammation Antibodies  >31 publications with TFAM and Inflammation
 Necrosis Antibodies  >27 publications with TFAM and Necrosis
 Liver Diseases Antibodies  >19 publications with TFAM and Liver Diseases
 Fatty Liver Antibodies  >16 publications with TFAM and Fatty Liver

Organs/Tissues associated with anti-TFAM antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >203 publications with TFAM and Muscle
 Heart Antibodies  >84 publications with TFAM and Heart
 Blood Antibodies  >76 publications with TFAM and Blood
 Brain Antibodies  >75 publications with TFAM and Brain
 Liver Antibodies  >74 publications with TFAM and Liver
 Connective Tissue Antibodies  >30 publications with TFAM and Connective Tissue
 Vascular Antibodies  >21 publications with TFAM and Vascular
 Lung Antibodies  >17 publications with TFAM and Lung
 Kidney Antibodies  >15 publications with TFAM and Kidney
 Thyroid Antibodies  >15 publications with TFAM and Thyroid
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