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USO1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS128118
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The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates… More details

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Product Overview

Online Datasheet in PDF
Product Name
USO1, Polyclonal Antibody
Full Product Name
USO1 Polyclonal Antibody
Product Gene Name
anti-USO1 antibody
Product Synonym Names
TAP; VDP; P115
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
962
OMIM
603344
3D Structure
ModBase 3D Structure for O60763
Species
Human
Route
Synthetic Peptide
Immunogen
A synthetic peptide of human USO1
Calculated Molecular Weight
108kDa

Product Categories/Family for anti-USO1 antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-USO1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-USO1 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50

Western Blot (WB) of anti-USO1 antibody

Western blot analysis of extracts of various cell lines, using USO1 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Rabbit Anti-USO1 Polyclonal Antibody – Cat# MBS128118

Immunohistochemistry (IHC) of anti-USO1 antibody

Immunohistochemistry of paraffin-embedded human kidney using USO1 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-USO1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS128118

Immunohistochemistry (IHC) of anti-USO1 antibody

Immunohistochemistry of paraffin-embedded human tonsil using USO1 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-USO1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS128118

Immunohistochemistry (IHC) of anti-USO1 antibody

Immunohistochemistry of paraffin-embedded human liver cancer using USO1 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-USO1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS128118

Immunohistochemistry (IHC) of anti-USO1 antibody

Immunohistochemistry of paraffin-embedded human gastric cancer using USO1 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-USO1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS128118

Immunohistochemistry (IHC) of anti-USO1 antibody

Immunohistochemistry of paraffin-embedded human uterine cancer using USO1 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-USO1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS128118

Other Notes

Small volumes of anti-USO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for USO1 purchase

MBS128118 is an antibody targeting USO1. Also known as USO1 Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for USO1. It may not necessarily be applicable to this product.

NCBI GI #
143743108
NCBI GeneID
8615
NCBI Accession #
O60763.2
UniProt Primary Accession #
O60763
UniProt Secondary Accession #
Q6PK63; Q86TB8; Q8N592; B2RAQ0
UniProt Related Accession #
O60763
NCBI Official Full Name
General vesicular transport factor p115
NCBI Official Synonym Full Names
USO1 vesicle transport factor
NCBI Official Symbol
USO1
NCBI Official Synonym Symbols
TAP; VDP; P115
UniProt Protein Name
General vesicular transport factor p115
UniProt Synonym Protein Names
Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking protein
Protein Family
General vesicular transport factor
UniProt Gene Name
USO1
UniProt Synonym Gene Names
VDP; TAP
UniProt Entry Name
USO1_HUMAN
NCBI Protein Information
general vesicular transport factor p115; vesicle docking protein p115; transcytosis associated protein; USO1 vesicle docking protein homolog

NCBI Summary for USO1

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
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UniProt Comments for USO1

TAP: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. Belongs to the VDP/USO1/EDE1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: Golgi membrane; Golgi apparatus; Golgi stack; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; nucleolus; cytosol

Molecular Function: protein transporter activity

Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; Golgi vesicle docking; vesicle fusion with Golgi apparatus; transcytosis; mitotic cell cycle
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Research Articles on USO1

1. P115 promotes proliferation of gastric cancer cells through an interaction with MIF.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-USO1 antibody

 Reference Product  PubMed Publications
 GORASP1 antibody  >1 publications with USO1 and GORASP1

Pathways associated with anti-USO1 antibody

 Products by Pathway  Pathway Diagram
 Arf1 Pathway antibodies  Arf1 Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Golgi Cisternae Pericentriolar Stack Reorganization Pathway antibodies  Golgi Cisternae Pericentriolar Stack Reorganization Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Prophase Pathway antibodies  Mitotic Prophase Pathway Diagram

Diseases associated with anti-USO1 antibody

 Disease Name  Pubmed Publications
 Embryo Loss Antibodies  >1 publications with USO1 and Embryo Loss
 Immune System Diseases Antibodies  >1 publications with USO1 and Immune System Diseases
 Liver Diseases Antibodies  >1 publications with USO1 and Liver Diseases
 Weight Loss Antibodies  >1 publications with USO1 and Weight Loss

Organs/Tissues associated with anti-USO1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >2 publications with USO1 and Liver
 Nerve Antibodies  >2 publications with USO1 and Nerve
 Thyroid Antibodies  >1 publications with USO1 and Thyroid
 Blood Antibodies  >1 publications with USO1 and Blood
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