Rabbit Anti-MAP2K5 Antibody – Cat# MBS128474

Western blot analysis of extracts from rat and mouse brain tissue using phospho-Map2k5-S311/T315 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

S311/T315 (MAP2K5) Antibody – Rabbit

Name: MAP2K5 Antibody; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS128474
Price: 440 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS128474

MBS128474 is an antibody targeting Map2k5. Also known as Phospho-Map2k5-S311/T315 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. kinase involved in signal transduction in the cell. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Map2k5-S311/T315, Antibody

Full Product Name

Phospho-Map2k5-S311/T315 Polyclonal Antibody

Product Gene Name

anti-Map2k5 antibody

Product Synonym Names

Phospho-Map2k5-S311/T315: Mek5

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

448

3D Structure

ModBase 3D Structure for Q62862

Species

Rat

Route

Synthetic Peptide

Immunogen

A phospho specific peptide corresponding to residues surrounding S311/T315 of human Map2k5

Calculated Molecular Weight

46kDa

Product Categories/Family for anti-Map2k5 antibody

Phosphorylation

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-Map2k5 antibody

Western Blot (WB)

Application Notes for anti-Map2k5 antibody

WB: 1:500 - 1:2000

Other Notes

Small volumes of anti-Map2k5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Map2k5. It may not necessarily be applicable to this product.

NCBI GI #

2499634

NCBI GeneID

29568

NCBI Accession #

Q62862.1

UniProt Primary Accession #

Q62862

UniProt Secondary Accession #

Q62863; Q62864

UniProt Related Accession #

Q62862

NCBI Official Full Name

Dual specificity mitogen-activated protein kinase kinase 5

NCBI Official Synonym Full Names

mitogen activated protein kinase kinase 5

NCBI Official Symbol

Map2k5

NCBI Official Synonym Symbols

Mek5

UniProt Protein Name

Dual specificity mitogen-activated protein kinase kinase 5

UniProt Synonym Protein Names

MAPK/ERK kinase 5; MEK 5

Protein Family

Dual specificity mitogen-activated protein kinase kinase

UniProt Gene Name

Map2k5

UniProt Synonym Gene Names

Mek5; Mkk5; Prkmk5; MAP kinase kinase 5; MAPKK 5; MEK 5

UniProt Entry Name

MP2K5_RAT

NCBI Protein Information

dual specificity mitogen-activated protein kinase kinase 5; MEK 5; MAPKK 5; MAPK/ERK kinase 5; MAP kinase kinase 5

NCBI Summary for Map2k5

kinase involved in signal transduction in the cell [RGD, Feb 2006]

UniProt Comments for Map2k5

MEK5: a dual specificity protein kinase of the STE7 family. Activates ERK5 and is itself phosphorylated and activated by MEKK3, as well as by atypical protein kinase C isoforms. Involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced isoforms have been described.

Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Cellular Component: membrane; spindle; intracellular; nucleus; cytosol

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; metal ion binding; protein-tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; negative regulation of heterotypic cell-cell adhesion; activation of MAPK activity; heart development; MAPKKK cascade; negative regulation of interleukin-8 biosynthetic process; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; positive regulation of protein metabolic process; positive regulation of cell growth; inhibition of NF-kappaB transcription factor; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation

Research Articles on Map2k5

1. The p38-mitogen-activated protein kinase activated protein kinase 2 (MK2) pathway is a component of the signaling cascade regulating oligodendrocyte differentiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Map2k5 antibody

Products by Pathway	Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
Gap Junction Pathway antibodies	Gap Junction Pathway Diagram
Gap Junction Pathway antibodies	Gap Junction Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Integrin-mediated Cell Adhesion Pathway antibodies	Integrin-mediated Cell Adhesion Pathway Diagram
MAPK (ERK5) Signaling Pathway antibodies	MAPK (ERK5) Signaling Pathway Diagram
MAPK (ERK5) Signaling Pathway antibodies	MAPK (ERK5) Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram

Diseases associated with anti-Map2k5 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>16 publications with Map2k5 and Nervous System Diseases
Body Weight Antibodies	>11 publications with Map2k5 and Body Weight
Hypertrophy Antibodies	>6 publications with Map2k5 and Hypertrophy
Kidney Diseases Antibodies	>5 publications with Map2k5 and Kidney Diseases
Cardiovascular Diseases Antibodies	>5 publications with Map2k5 and Cardiovascular Diseases
Necrosis Antibodies	>4 publications with Map2k5 and Necrosis
Inflammation Antibodies	>3 publications with Map2k5 and Inflammation
Hyperplasia Antibodies	>3 publications with Map2k5 and Hyperplasia
Heart Diseases Antibodies	>3 publications with Map2k5 and Heart Diseases
Brain Diseases Antibodies	>2 publications with Map2k5 and Brain Diseases

Organs/Tissues associated with anti-Map2k5 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>13 publications with Map2k5 and Brain
Muscle Antibodies	>11 publications with Map2k5 and Muscle
Prostate Antibodies	>7 publications with Map2k5 and Prostate
Lung Antibodies	>3 publications with Map2k5 and Lung
Artery Antibodies	>3 publications with Map2k5 and Artery
Connective Tissue Antibodies	>2 publications with Map2k5 and Connective Tissue
Embryonic Tissue Antibodies	>2 publications with Map2k5 and Embryonic Tissue
Eye Antibodies	>2 publications with Map2k5 and Eye