Rabbit Anti-EIF2S2 Antibody – Cat# MBS128500

Western blot analysis of extracts from C2C12 cells, using Phospho-EIF2S2-S2 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

EIF2S2 Antibody – Rabbit

Name: EIF2S2 Antibody; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS128500
Price: 440 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS128500

Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma,… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

EIF2S2-S2, Antibody

Full Product Name

Phospho-EIF2S2-S2 Polyclonal Antibody

Product Gene Name

anti-EIF2S2 antibody

Product Synonym Names

Phospho-EIF2S2-S2: EIF2; EIF2B; PPP1R67; EIF2beta; eIF-2-beta

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

333

OMIM

603908

3D Structure

ModBase 3D Structure for P20042

Species

Human

Route

Synthetic Peptide

Immunogen

A phospho specific peptide corresponding to residues surrounding S2 of human EIF2S2

Calculated Molecular Weight

38kDa

Product Categories/Family for anti-EIF2S2 antibody

Phosphorylation

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-EIF2S2 antibody

Western Blot (WB)

Application Notes for anti-EIF2S2 antibody

WB: 1:500 - 1:2000

Other Notes

Small volumes of anti-EIF2S2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF2S2 purchase

MBS128500 is an antibody targeting EIF2S2. Also known as Phospho-EIF2S2-S2 Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF2S2. It may not necessarily be applicable to this product.

NCBI GI #

12644154

NCBI GeneID

8894

NCBI Accession #

P20042.2

UniProt Primary Accession #

P20042

UniProt Secondary Accession #

Q9BVU0; Q9UJE4

UniProt Related Accession #

P20042

NCBI Official Full Name

Eukaryotic translation initiation factor 2 subunit 2

NCBI Official Synonym Full Names

eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa

NCBI Official Symbol

EIF2S2

NCBI Official Synonym Symbols

EIF2; EIF2B; PPP1R67; EIF2beta; eIF-2-beta

UniProt Protein Name

Eukaryotic translation initiation factor 2 subunit 2

UniProt Synonym Protein Names

Eukaryotic translation initiation factor 2 subunit beta; eIF-2-beta

Protein Family

Eukaryotic translation initiation factor

UniProt Gene Name

EIF2S2

UniProt Synonym Gene Names

EIF2B; eIF-2-beta

UniProt Entry Name

IF2B_HUMAN

NCBI Protein Information

eukaryotic translation initiation factor 2 subunit 2; protein phosphatase 1, regulatory subunit 67; eukaryotic translation initiation factor 2 subunit beta

UniProt Comments for EIF2S2

eIF2-beta: a translational regulatory protein that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: cytoplasm; nucleus; cytosol; eukaryotic translation initiation factor 2 complex

Molecular Function: protein binding; RNA binding; translation factor activity, nucleic acid binding; translation initiation factor activity; metal ion binding

Biological Process: cellular protein metabolic process; translation; in utero embryonic development; male gonad development; translational initiation; gene expression

Research Articles on EIF2S2

1. This study provides mechanistic insight into the role of eIF5-carboxyl terminal domain's dynamic interplay with eIF1 and eIF2beta

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EIF2S2 antibody

Reference Product	PubMed Publications
EIF2S1 antibody	>1 publications with EIF2S2 and EIF2S1

Pathways associated with anti-EIF2S2 antibody

Products by Pathway	Pathway Diagram
Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies	Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
BDNF Signaling Pathway antibodies	BDNF Signaling Pathway Diagram
Cap-dependent Translation Initiation Pathway antibodies	Cap-dependent Translation Initiation Pathway Diagram
Eukaryotic Translation Initiation Pathway antibodies	Eukaryotic Translation Initiation Pathway Diagram
Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway antibodies	Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway Diagram
GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies	GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
L13a-mediated Translational Silencing Of Ceruloplasmin Expression Pathway antibodies	L13a-mediated Translational Silencing Of Ceruloplasmin Expression Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
RNA Transport Pathway antibodies	RNA Transport Pathway Diagram

Diseases associated with anti-EIF2S2 antibody

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>1 publications with EIF2S2 and Breast Neoplasms

Organs/Tissues associated with anti-EIF2S2 antibody

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>1 publications with EIF2S2 and Testis