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RPS6KB1 Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS128559
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This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene… More details

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Product Overview

Product Name
RPS6KB1-S424, Antibody
Full Product Name
Phospho-RPS6KB1-S424 Polyclonal Antibody
Product Gene Name
anti-RPS6KB1 antibody
Product Synonym Names
Phospho-RPS6KB1-S424: S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
525
OMIM
608938
3D Structure
ModBase 3D Structure for P23443
Species
Human
Route
Synthetic Peptide
Immunogen
A phospho specific peptide corresponding to residues surrounding S424 of human RPS6KB1
Calculated Molecular Weight
56-59kDa

Product Categories/Family for anti-RPS6KB1 antibody

Phosphorylation
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-RPS6KB1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-RPS6KB1 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:100 - 1:200

Western Blot (WB) of anti-RPS6KB1 antibody

Western blot analysis of extracts from MCF cells using Phospho-RPS6KB1-S424 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Rabbit Anti-RPS6KB1 Antibody – Cat# MBS128559

Immunohistochemistry (IHC) of anti-RPS6KB1 antibody

Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-RPS6KB1-S424 antibody.
Rabbit Anti-RPS6KB1 Antibody, Immunohistochemistry (IHC) – Cat# MBS128559

Immunofluorescence (IF) of anti-RPS6KB1 antibody

Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, centrosomal and nuclear staining using Phospho-RPS6KB1-S424 antibody.
Rabbit Anti-RPS6KB1 Antibody, Immunofluorescence (IF) – Cat# MBS128559

Other Notes

Small volumes of anti-RPS6KB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPS6KB1 purchase

MBS128559 is an antibody targeting RPS6KB1. Also known as Phospho-RPS6KB1-S424 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RPS6KB1. It may not necessarily be applicable to this product.

NCBI GI #
54041234
NCBI GeneID
6198
NCBI Accession #
P23443.2
UniProt Primary Accession #
P23443
UniProt Secondary Accession #
Q7Z721; B2R779; B4DLT4; B4DTG1; E7ESB8; F6UYM1
UniProt Related Accession #
P23443
NCBI Official Full Name
Ribosomal protein S6 kinase beta-1
NCBI Official Synonym Full Names
ribosomal protein S6 kinase, 70kDa, polypeptide 1
NCBI Official Symbol
RPS6KB1
NCBI Official Synonym Symbols
S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
UniProt Protein Name
Ribosomal protein S6 kinase beta-1
UniProt Synonym Protein Names
70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA
Protein Family
Ribosomal protein S6 kinase
UniProt Gene Name
RPS6KB1
UniProt Synonym Gene Names
STK14A; S6K-beta-1; S6K1; P70S6K1; p70-S6K 1; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA
UniProt Entry Name
KS6B1_HUMAN
NCBI Protein Information
ribosomal protein S6 kinase beta-1; p70 S6 kinase, alpha; ribosomal protein S6 kinase I; serine/threonine kinase 14 alpha; serine/threonine-protein kinase 14A

UniProt Comments for RPS6KB1

p70S6K: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported.

Protein type: Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Translation; AGC group; RSK family; p70 subfamily

Chromosomal Location of Human Ortholog: 17q23.1
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Research Articles on RPS6KB1

1. Data indicate that zinc finger protein SNAIL modulates ribosomal protein S6 kinase 1 (RPS6KB1)/HIF-1alpha/protein kinase 2 (PKM2) signaling pathway through miR-128.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPS6KB1 antibody

 Reference Product  PubMed Publications
 MTOR antibody  >38 publications with RPS6KB1 and MTOR
 PIK3CA antibody  >10 publications with RPS6KB1 and PIK3CA
 RPS6 antibody  >8 publications with RPS6KB1 and RPS6
 IRS1 antibody  >4 publications with RPS6KB1 and IRS1
 RHEB antibody  >2 publications with RPS6KB1 and RHEB
 EIF4B antibody  >1 publications with RPS6KB1 and EIF4B
 RPTOR antibody  >1 publications with RPS6KB1 and RPTOR

Pathways associated with anti-RPS6KB1 antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram

Diseases associated with anti-RPS6KB1 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >13 publications with RPS6KB1 and Breast Neoplasms
 Adenocarcinoma Antibodies  >6 publications with RPS6KB1 and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >5 publications with RPS6KB1 and Neoplasms, Experimental
 Inflammation Antibodies  >5 publications with RPS6KB1 and Inflammation
 Cardiovascular Diseases Antibodies  >3 publications with RPS6KB1 and Cardiovascular Diseases
 Ovarian Neoplasms Antibodies  >3 publications with RPS6KB1 and Ovarian Neoplasms
 Lung Neoplasms Antibodies  >2 publications with RPS6KB1 and Lung Neoplasms
 Liver Diseases Antibodies  >2 publications with RPS6KB1 and Liver Diseases
 Pancreatic Neoplasms Antibodies  >2 publications with RPS6KB1 and Pancreatic Neoplasms
 Lymphoma, Large B-Cell, Diffuse Antibodies  >2 publications with RPS6KB1 and Lymphoma, Large B-Cell, Diffuse

Organs/Tissues associated with anti-RPS6KB1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >5 publications with RPS6KB1 and Muscle
 Liver Antibodies  >5 publications with RPS6KB1 and Liver
 Brain Antibodies  >5 publications with RPS6KB1 and Brain
 Blood Antibodies  >3 publications with RPS6KB1 and Blood
 Lung Antibodies  >2 publications with RPS6KB1 and Lung
 Prostate Antibodies  >2 publications with RPS6KB1 and Prostate
 Pancreas Antibodies  >2 publications with RPS6KB1 and Pancreas
 Heart Antibodies  >2 publications with RPS6KB1 and Heart
 Intestine Antibodies  >2 publications with RPS6KB1 and Intestine
 Eye Antibodies  >1 publications with RPS6KB1 and Eye
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