Rabbit Anti-HLA-F Polyclonal Antibody – Cat# MBS128731

Western blot analysis of extracts of HL-60 cells, using HLA-F antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

HLA-F Antibody – Rabbit Polyclonal

Name: HLA-F Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS128731
Price: 360 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS128731

This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

HLA-F, Polyclonal Antibody

Full Product Name

HLA-F Polyclonal Antibody

Product Gene Name

anti-HLA-F antibody

Product Synonym Names

HLAF; CDA12; HLA-5.4; HLA-CDA12

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

346

OMIM

143110

3D Structure

ModBase 3D Structure for P30511

Species

Human

Immunogen

Recombinant protein of human HLA-F

Calculated Molecular Weight

39kDa

Product Categories/Family for anti-HLA-F antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-HLA-F antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-HLA-F antibody

WB: 1:200-1:2000
IHC: 1:50-1:200

Other Notes

Small volumes of anti-HLA-F antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HLA-F purchase

MBS128731 is an antibody targeting HLA-F. Also known as HLA-F Polyclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HLA-F. It may not necessarily be applicable to this product.

NCBI GI #

317373438

NCBI GeneID

3134

NCBI Accession #

P30511.3

UniProt Primary Accession #

P30511

UniProt Secondary Accession #

Q5JQI8; Q5JQJ1; Q5SPT5; Q860R0; Q8MGQ1; Q8WLP5; Q95HC0; Q9TP68

UniProt Related Accession #

P30511

NCBI Official Full Name

HLA class I histocompatibility antigen, alpha chain F

NCBI Official Synonym Full Names

major histocompatibility complex, class I, F

NCBI Official Symbol

HLA-F

NCBI Official Synonym Symbols

HLAF; CDA12; HLA-5.4; HLA-CDA12

UniProt Protein Name

HLA class I histocompatibility antigen, alpha chain F

UniProt Synonym Protein Names

CDA12; HLA F antigen; Leukocyte antigen F; MHC class I antigen F

Protein Family

HLA class I histocompatibility antigen

UniProt Gene Name

HLA-F

UniProt Synonym Gene Names

HLA-5.4; HLAF

UniProt Entry Name

HLAF_HUMAN

NCBI Protein Information

HLA class I histocompatibility antigen, alpha chain F; HLA F antigen; leukocyte antigen F; HLA class I molecule; MHC class Ib antigen; MHC class I antigen F

UniProt Comments for HLA-F

HLA-F: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; phagocytic vesicle membrane; cell surface; membrane; early endosome membrane; endoplasmic reticulum; plasma membrane; MHC class I protein complex

Molecular Function: peptide antigen binding; TAP1 binding; TAP2 binding; receptor binding

Biological Process: regulation of immune response; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I

Research Articles on HLA-F

1. A previously unrecognized model of Ag cross-presentation mediated by HLA-F & MHC-I open conformers on activated lymphocytes & monocytes may significantly contribute to the regulation of immune system functions & the immune defense.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HLA-F antibody

Reference Product	PubMed Publications
HLA-E antibody	>60 publications with HLA-F and HLA-E
HLA-A antibody	>57 publications with HLA-F and HLA-A
HLA-B antibody	>30 publications with HLA-F and HLA-B
HLA-C antibody	>18 publications with HLA-F and HLA-C
TAP1 antibody	>3 publications with HLA-F and TAP1
LILRB1 antibody	>2 publications with HLA-F and LILRB1
LILRB2 antibody	>2 publications with HLA-F and LILRB2
B2M antibody	>1 publications with HLA-F and B2M

Pathways associated with anti-HLA-F antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies	Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Antigen Processing-Cross Presentation Pathway antibodies	Antigen Processing-Cross Presentation Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram

Diseases associated with anti-HLA-F antibody

Disease Name	Pubmed Publications
Carcinoma Antibodies	>14 publications with HLA-F and Carcinoma
Nervous System Diseases Antibodies	>8 publications with HLA-F and Nervous System Diseases
Skin Diseases Antibodies	>7 publications with HLA-F and Skin Diseases
Cardiovascular Diseases Antibodies	>6 publications with HLA-F and Cardiovascular Diseases
Carcinoma, Hepatocellular Antibodies	>5 publications with HLA-F and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>5 publications with HLA-F and Liver Diseases
Neoplasm Metastasis Antibodies	>4 publications with HLA-F and Neoplasm Metastasis
Adenocarcinoma Antibodies	>4 publications with HLA-F and Adenocarcinoma
Breast Neoplasms Antibodies	>3 publications with HLA-F and Breast Neoplasms
Inflammation Antibodies	>3 publications with HLA-F and Inflammation

Organs/Tissues associated with anti-HLA-F antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>37 publications with HLA-F and Blood
Liver Antibodies	>12 publications with HLA-F and Liver
Bone Antibodies	>5 publications with HLA-F and Bone
Brain Antibodies	>5 publications with HLA-F and Brain
Uterus Antibodies	>4 publications with HLA-F and Uterus
Bone Marrow Antibodies	>4 publications with HLA-F and Bone Marrow
Skin Antibodies	>4 publications with HLA-F and Skin
Muscle Antibodies	>3 publications with HLA-F and Muscle
Testis Antibodies	>3 publications with HLA-F and Testis
Tonsil Antibodies	>2 publications with HLA-F and Tonsil