Rabbit Anti-SF3A3 Polyclonal Antibody – Cat# MBS128776

Western blot analysis of extracts of various cell lines, using SF3A3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

SF3A3 Antibody – Rabbit Polyclonal

Name: SF3A3 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS128776
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS128776

This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing.… More details

$225 / 0.02 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SF3A3, Polyclonal Antibody

Full Product Name

SF3A3 Polyclonal Antibody

Product Gene Name

anti-SF3A3 antibody

Product Synonym Names

PRP9; PRPF9; SAP61; SF3a60

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

PBS with 0.09% Sodium azide,50% glycerol,pH7.3.

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

501

OMIM

605596

3D Structure

ModBase 3D Structure for Q12874

Species

Human

Immunogen

Recombinant protein of human SF3A3

Calculated Molecular Weight

59kDa

Product Categories/Family for anti-SF3A3 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-SF3A3 antibody

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Application Notes for anti-SF3A3 antibody

WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
IP: 1:20 - 1:50

Other Notes

Small volumes of anti-SF3A3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SF3A3 purchase

MBS128776 is an antibody targeting SF3A3. Also known as SF3A3 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SF3A3. It may not necessarily be applicable to this product.

NCBI GI #

17380310

NCBI GeneID

10946

NCBI Accession #

Q12874.1

UniProt Primary Accession #

Q12874

UniProt Secondary Accession #

Q15460; Q5VT87; D3DPT5

UniProt Related Accession #

Q12874

NCBI Official Full Name

Splicing factor 3A subunit 3

NCBI Official Synonym Full Names

splicing factor 3a, subunit 3, 60kDa

NCBI Official Symbol

SF3A3

NCBI Official Synonym Symbols

PRP9; PRPF9; SAP61; SF3a60

UniProt Protein Name

Splicing factor 3A subunit 3

UniProt Synonym Protein Names

SF3a60; Spliceosome-associated protein 61; SAP 61

UniProt Gene Name

SF3A3

UniProt Synonym Gene Names

SAP61; SAP 61

UniProt Entry Name

SF3A3_HUMAN

NCBI Protein Information

splicing factor 3A subunit 3; SAP 61; spliceosome associated protein 61; spliceosome-associated protein 61; splicing factor 3a, subunit 3, 60kD; pre-mRNA splicing factor SF3a (60kD)

UniProt Comments for SF3A3

SF3A3: subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

Protein type: Spliceosome; Motility/polarity/chemotaxis; RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; spliceosome; nuclear speck

Molecular Function: protein binding; zinc ion binding

Biological Process: nuclear mRNA 3'-splice site recognition; RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA processing

Research Articles on SF3A3

1. SF3a3 functions as a co-repressor of Constitutive Androstane Receptor transcriptional activity, in addition to its canonical function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SF3A3 antibody

Products by Pathway	Pathway Diagram
Diurnally Regulated Genes With Circadian Orthologs Pathway antibodies	Diurnally Regulated Genes With Circadian Orthologs Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies	Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome, U2-snRNP Pathway antibodies	Spliceosome, U2-snRNP Pathway Diagram
Spliceosome, U2-snRNP Pathway antibodies	Spliceosome, U2-snRNP Pathway Diagram
MRNA Splicing Pathway antibodies	MRNA Splicing Pathway Diagram
MRNA Splicing - Major Pathway antibodies	MRNA Splicing - Major Pathway Diagram
MRNA Processing Pathway antibodies	MRNA Processing Pathway Diagram

Organs/Tissues associated with anti-SF3A3 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>1 publications with SF3A3 and Liver
Brain Antibodies	>1 publications with SF3A3 and Brain