Rabbit Anti-YARS2 Polyclonal Antibody – Cat# MBS129028

Western blot analysis of extracts of various cell lines, using YARS2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

YARS2 Antibody – Rabbit Polyclonal

Name: YARS2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS129028
Price: 360 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS129028

This gene encodes a mitochondrial protein that catalyzes the attachment of tyrosine to tRNA(Tyr). Mutations in this gene are associated with myopathy with lactic acidosis and sideroblastic anemia type 2 (MLASA2). More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

YARS2, Polyclonal Antibody

Full Product Name

YARS2 Polyclonal Antibody

Product Gene Name

anti-YARS2 antibody

Product Synonym Names

TYRRS; CGI-04; MLASA2; MT-TYRRS

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

477

OMIM

gene 613561

3D Structure

ModBase 3D Structure for Q9Y2Z4

Species

Human

Immunogen

Recombinant protein of human YARS2

Calculated Molecular Weight

53kDa

Product Categories/Family for anti-YARS2 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-YARS2 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-YARS2 antibody

WB: 1:500-1:2000
IHC: 1:50-1:200

Other Notes

Small volumes of anti-YARS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for YARS2 purchase

MBS129028 is an antibody targeting YARS2. Also known as YARS2 Polyclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for YARS2. It may not necessarily be applicable to this product.

NCBI GI #

50401709

NCBI GeneID

51067

NCBI Accession #

Q9Y2Z4.2

UniProt Primary Accession #

Q9Y2Z4

UniProt Secondary Accession #

Q9H817; D3DUW8

UniProt Related Accession #

Q9Y2Z4

NCBI Official Full Name

Tyrosine--tRNA ligase, mitochondrial

NCBI Official Synonym Full Names

tyrosyl-tRNA synthetase 2, mitochondrial

NCBI Official Symbol

YARS2

NCBI Official Synonym Symbols

TYRRS; CGI-04; MLASA2; MT-TYRRS

UniProt Protein Name

Tyrosine--tRNA ligase, mitochondrial

UniProt Synonym Protein Names

Tyrosyl-tRNA synthetase; TyrRS

UniProt Gene Name

YARS2

UniProt Synonym Gene Names

TyrRS

UniProt Entry Name

SYYM_HUMAN

NCBI Protein Information

tyrosine--tRNA ligase, mitochondrial; tyrosine tRNA ligase 2, mitochondrial

UniProt Comments for YARS2

YARS2: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr). Defects in YARS2 are the cause of myopathy with lactic acidosis and sideroblastic anemia type 2 (MLASA2). MLASA2 is a rare oxidative phosphorylation disorder specific to skeletal muscle and bone marrow. Affected individuals manifest sideroblastic anemia, progressive lethargy, muscle weakness, and exercise intolerance associated with persistent lactic acidemia. Belongs to the class-I aminoacyl-tRNA synthetase family.

Protein type: RNA-binding; Ligase; Translation; Mitochondrial; EC 6.1.1.1

Chromosomal Location of Human Ortholog: 12p11.21

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: protein homodimerization activity; tyrosine-tRNA ligase activity; ATP binding; tRNA binding

Biological Process: tRNA aminoacylation for protein translation; translation; gene expression; tRNA aminoacylation

Disease: Myopathy, Lactic Acidosis, And Sideroblastic Anemia 2

Research Articles on YARS2

1. Data identified novel YARS2 mutations and noted marked phenotypic variability among YARS2 MLASA patients, with phenotypes ranging from mild to lethal, suggesting that the background mtDNA haplotype may be contributing to the phenotypic variability.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-YARS2 antibody

Reference Product	PubMed Publications
PUS1 antibody	>4 publications with YARS2 and PUS1

Pathways associated with anti-YARS2 antibody

Products by Pathway	Pathway Diagram
Aminoacyl-tRNA Biosynthesis Pathway antibodies	Aminoacyl-tRNA Biosynthesis Pathway Diagram
Aminoacyl-tRNA Biosynthesis Pathway antibodies	Aminoacyl-tRNA Biosynthesis Pathway Diagram
Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies	Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies	Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Mitochondrial TRNA Aminoacylation Pathway antibodies	Mitochondrial TRNA Aminoacylation Pathway Diagram
TRNA Aminoacylation Pathway antibodies	TRNA Aminoacylation Pathway Diagram

Diseases associated with anti-YARS2 antibody

Disease Name	Pubmed Publications
Anemia Antibodies	>7 publications with YARS2 and Anemia
Myopathy with lactic acidosis and sideroblastic anemia Antibodies	>6 publications with YARS2 and Myopathy with lactic acidosis and sideroblastic anemia

Organs/Tissues associated with anti-YARS2 antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>2 publications with YARS2 and Bone
Bone Marrow Antibodies	>2 publications with YARS2 and Bone Marrow
Testis Antibodies	>1 publications with YARS2 and Testis