Rabbit Anti-GART Polyclonal Antibody – Cat# MBS129135

Western blot analysis of extracts of various cell lines, using GART antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

GART Antibody – Rabbit Polyclonal

Name: GART Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS129135
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS129135

The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This… More details

$225 / 0.02 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GART, Polyclonal Antibody

Full Product Name

GART Polyclonal Antibody

Product Gene Name

anti-GART antibody

Product Synonym Names

AIRS; GARS; PAIS; PGFT; PRGS; GARTF

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

1010

OMIM

138440

3D Structure

ModBase 3D Structure for P22102

Species

Human

Immunogen

Recombinant protein of human GART

Calculated Molecular Weight

108kDa

Product Categories/Family for anti-GART antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-GART antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-GART antibody

WB: 1:500-1:2000
IHC: 1:50-1:200

Other Notes

Small volumes of anti-GART antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GART purchase

MBS129135 is an antibody targeting GART. Also known as GART Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GART. It may not necessarily be applicable to this product.

NCBI GI #

131616

NCBI GeneID

2618

NCBI Accession #

P22102.1

UniProt Primary Accession #

P22102

UniProt Secondary Accession #

O14659; Q52M77; A8K945; A8KA32; D3DSF3; D3DSF4

UniProt Related Accession #

P22102

NCBI Official Full Name

Trifunctional purine biosynthetic protein adenosine-3

NCBI Official Synonym Full Names

phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase

NCBI Official Symbol

GART

NCBI Official Synonym Symbols

AIRS; GARS; PAIS; PGFT; PRGS; GARTF

UniProt Protein Name

Trifunctional purine biosynthetic protein adenosine-3

UniProt Synonym Protein Names

Glycinamide ribonucleotide synthetase; GARS; Phosphoribosylglycinamide synthetase

Protein Family

Phosphoribosylglycinamide formyltransferase

UniProt Gene Name

GART

UniProt Synonym Gene Names

PGFT; PRGS; GARS; AIRS; GART

UniProt Entry Name

PUR2_HUMAN

NCBI Protein Information

trifunctional purine biosynthetic protein adenosine-3

UniProt Comments for GART

GART: a highly conserved trifunctional enzyme. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. Inhibition of the de novo purine synthesis by folate analogs blocks the p53-dependent G1 checkpoint in human carcinoma cell lines. Two splice variant isoforms have been described.

Protein type: Methyltransferase; Ligase; EC 6.3.3.1; Nucleotide Metabolism - purine; EC 2.1.2.2; EC 6.3.4.13; Cofactor and Vitamin Metabolism - one carbon pool by folate; Mitochondrial; Enzyme, cellular metabolism

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: cytosol

Molecular Function: methyltransferase activity; metal ion binding; phosphoribosylamine-glycine ligase activity; phosphoribosylformylglycinamidine cyclo-ligase activity; phosphoribosylglycinamide formyltransferase activity; ATP binding

Biological Process: methylation; response to organic substance; purine ribonucleoside monophosphate biosynthetic process; glycine metabolic process; tetrahydrofolate biosynthetic process; response to inorganic substance; purine base biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; cerebellum development; cerebral cortex development; purine base metabolic process; 'de novo' IMP biosynthetic process

Research Articles on GART

1. The current results showed that GART expression was associated with glioma grade and that high GART protein expression might be related to poor outcome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GART antibody

Reference Product	PubMed Publications
ATIC antibody	>3 publications with GART and ATIC
PAICS antibody	>3 publications with GART and PAICS
PPAT antibody	>2 publications with GART and PPAT
PFAS antibody	>1 publications with GART and PFAS

Pathways associated with anti-GART antibody

Products by Pathway	Pathway Diagram
5-aminoimidazole Ribonucleotide Biosynthesis Pathway antibodies	5-aminoimidazole Ribonucleotide Biosynthesis Pathway Diagram
5-aminoimidazole Ribonucleotide Biosynthesis I Pathway antibodies	5-aminoimidazole Ribonucleotide Biosynthesis I Pathway Diagram
Inosine Monophosphate Biosynthesis, PRPP + Glutamine => IMP Pathway antibodies	Inosine Monophosphate Biosynthesis, PRPP + Glutamine => IMP Pathway Diagram
Inosine Monophosphate Biosynthesis, PRPP + Glutamine => IMP Pathway antibodies	Inosine Monophosphate Biosynthesis, PRPP + Glutamine => IMP Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nucleotides Pathway antibodies	Metabolism Of Nucleotides Pathway Diagram
One Carbon Metabolism Pathway antibodies	One Carbon Metabolism Pathway Diagram
One Carbon Pool By Folate Pathway antibodies	One Carbon Pool By Folate Pathway Diagram
One Carbon Pool By Folate Pathway antibodies	One Carbon Pool By Folate Pathway Diagram

Diseases associated with anti-GART antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>21 publications with GART and Nervous System Diseases
Congenital Abnormalities Antibodies	>13 publications with GART and Congenital Abnormalities
Breast Neoplasms Antibodies	>7 publications with GART and Breast Neoplasms
Neoplasms, Experimental Antibodies	>7 publications with GART and Neoplasms, Experimental
Disease Models, Animal Antibodies	>6 publications with GART and Disease Models, Animal
Cardiovascular Diseases Antibodies	>4 publications with GART and Cardiovascular Diseases
Liver Diseases Antibodies	>4 publications with GART and Liver Diseases
Inflammation Antibodies	>3 publications with GART and Inflammation
Carcinoma, Hepatocellular Antibodies	>2 publications with GART and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>2 publications with GART and Liver Neoplasms

Organs/Tissues associated with anti-GART antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>24 publications with GART and Blood
Liver Antibodies	>8 publications with GART and Liver
Ovary Antibodies	>8 publications with GART and Ovary
Brain Antibodies	>8 publications with GART and Brain
Bone Antibodies	>7 publications with GART and Bone
Skin Antibodies	>6 publications with GART and Skin
Lung Antibodies	>4 publications with GART and Lung
Muscle Antibodies	>4 publications with GART and Muscle
Bone Marrow Antibodies	>4 publications with GART and Bone Marrow
Vascular Antibodies	>3 publications with GART and Vascular