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E3 Ubiquitin-Protein Ligase HUWE1 (HUWE1) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1291431

This gene encodes a member of the HECT E3 ubiquitin ligase family. The HECT domain lies in the C-terminus and contains the active-site cysteine which forms an intermediate ubiquitin-thioester bond. E3 family members are divided into three subfamilies based on… More details

$405 / 0.02 mg (E-Coli) +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
E3 ubiquitin-protein ligase HUWE1 (HUWE1), Recombinant Protein
Full Product Name
Recombinant Human E3 ubiquitin-protein ligase HUWE1 (HUWE1) , partial
Product Gene Name
HUWE1 recombinant protein
Product Synonym Gene Name
HUWE1
Sequence
LERLDEGLRK EDMAVHVRRD HVFEDSYREL HRKSPEEMKN RLYIVFEGEE GQDAGGLLRE WYMIISREMF NPMYALFRTS PGDRVTYTIN PSSHCNPNHL SYFKFVGRIV AKAVYDNRLL ECYFTRSFYK HILGKSVRYT DMESEDYHFY QGLVYLLEND VSTLGYDLTF STEVQEFGVC EVRDLKPNGA NILVTEENKK EYVHLVCQMR MTGAIRKQLA AFLEGFYEII PKRLISIFTE QELELLISGL PTIDIDDLKS NTEYHKYQSN SIQIQWFWRA LRSFDQADRA KFLQFVTGTS KVPLQGFAAL EGMNGIQKFQ IHRDDRSTDR LPSAHTCFNQ LDLPAYESFE KLRHMLLLAI QECSEGFGLA
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
481,018 Da
Sequence Positions
4005-4374aa; Partial
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
OMIM
300697
3D Structure
ModBase 3D Structure for Q7Z6Z7
Species
Human
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of HUWE1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HUWE1 purchase

MBS1291431 is a recombinant protein targeting HUWE1. Also known as Recombinant Human E3 ubiquitin-protein ligase HUWE1 (HUWE1) , partial. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for HUWE1. It may not necessarily be applicable to this product.

NCBI GI #
61676188
NCBI GeneID
10075
NCBI Accession #
NP_113584.3
UniProt Primary Accession #
Q7Z6Z7
UniProt Secondary Accession #
O15029; Q4G2Z2; Q5H961; Q6P4D0; Q8NG67; Q9BUI0; Q9HCJ4; Q9NSL6; Q9P0A9
UniProt Related Accession #
Q7Z6Z7
GenBank Nucleotide #
NM_031407.6
NCBI Official Full Name
E3 ubiquitin-protein ligase HUWE1
NCBI Official Synonym Full Names
HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
NCBI Official Symbol
HUWE1
NCBI Official Synonym Symbols
MULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272
UniProt Protein Name
E3 ubiquitin-protein ligase HUWE1
UniProt Synonym Protein Names
ARF-binding protein 1; ARF-BP1; HECT, UBA and WWE domain-containing protein 1; HECT-type E3 ubiquitin transferase HUWE1; Homologous to E6AP carboxyl terminus homologous protein 9; HectH9; Large structure of UREB1; LASU1; Mcl-1 ubiquitin ligase E3; Mule; Upstream regulatory element-binding protein 1; URE-B1; URE-binding protein 1
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
HUWE1
UniProt Synonym Gene Names
KIAA0312; KIAA1578; UREB1; ARF-BP1; HectH9; LASU1; Mule; URE-B1; URE-binding protein 1
NCBI Protein Information
E3 ubiquitin-protein ligase HUWE1

NCBI Summary for HUWE1

This gene encodes a protein containing a C-terminal HECT (E6AP type E3 ubiquitin protein ligase) domain that functions as an E3 ubiquitin ligase. The encoded protein is required for the ubiquitination and subsequent degradation of the anti-apoptotic protein Mcl1 (myeloid cell leukemia sequence 1 (BCL2-related)). This protein also ubiquitinates the p53 tumor suppressor, core histones, and DNA polymerase beta. Mutations in this gene are associated with Turner type X-linked syndromic cognitive disability. [provided by RefSeq, Aug 2013]
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UniProt Comments for HUWE1

E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Mediates polyubiquitination of isoform 2 of PA2G4.
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Research Articles on HUWE1

1. The authors show that HUWE1 dimerizes in solution and self-associates in cells, and that both occurs through the crystallographic dimer interface. They also demonstrate that HUWE1 is inhibited in cells and that it can be activated by disruption of the dimer interface.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HUWE1 recombinant protein

 Reference Product  PubMed Publications
 MCL1 recombinant protein  >2 publications with HUWE1 and MCL1
 TP53 recombinant protein  >1 publications with HUWE1 and TP53

Pathways associated with HUWE1 recombinant protein

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Validated Targets Of C-MYC Transcriptional Activation Pathway antibodies  Validated Targets Of C-MYC Transcriptional Activation Pathway Diagram
 P53 Pathway antibodies  P53 Pathway Diagram

Diseases associated with HUWE1 recombinant protein

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >9 publications with HUWE1 and Nervous System Diseases
 Breast Neoplasms Antibodies  >6 publications with HUWE1 and Breast Neoplasms
 Skin Diseases Antibodies  >6 publications with HUWE1 and Skin Diseases
 Carcinoma Antibodies  >6 publications with HUWE1 and Carcinoma
 Carcinoma, Hepatocellular Antibodies  >4 publications with HUWE1 and Carcinoma, Hepatocellular
 Neoplasms, Experimental Antibodies  >3 publications with HUWE1 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >3 publications with HUWE1 and Cell Transformation, Neoplastic
 Liver Neoplasms Antibodies  >3 publications with HUWE1 and Liver Neoplasms
 Liver Diseases Antibodies  >3 publications with HUWE1 and Liver Diseases
 Liver Neoplasms, Experimental Antibodies  >2 publications with HUWE1 and Liver Neoplasms, Experimental

Organs/Tissues associated with HUWE1 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with HUWE1 and Brain
 Blood Antibodies  >4 publications with HUWE1 and Blood
 Embryonic Tissue Antibodies  >4 publications with HUWE1 and Embryonic Tissue
 Liver Antibodies  >4 publications with HUWE1 and Liver
 Vascular Antibodies  >3 publications with HUWE1 and Vascular
 Testis Antibodies  >3 publications with HUWE1 and Testis
 Muscle Antibodies  >3 publications with HUWE1 and Muscle
 Kidney Antibodies  >2 publications with HUWE1 and Kidney
 Pancreas Antibodies  >2 publications with HUWE1 and Pancreas
 Lung Antibodies  >2 publications with HUWE1 and Lung
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