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ATPIF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS129181
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This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified.Background: Enables several functions, including ATPase binding activity; angiostatin… More details

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Product Overview

Online Datasheet in PDF
Product Name
ATPIF1, Polyclonal Antibody
Full Product Name
ATPIF1 Polyclonal Antibody
Product Gene Name
anti-ATPIF1 antibody
Product Synonym Names
IP; ATPI; ATPIP; ATPIF1; F1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Molecular Weight
Observed MW: 12 kDa
Calculated MW: 12 KDa
OMIM
614981
3D Structure
ModBase 3D Structure for Q9UII2
Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 26-106 of human ATPIF1 (NP_057395.1).
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Product Categories/Family for anti-ATPIF1 antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ATPIF1 antibody
WB, IF,ICC, IP, ELISA.
Application Notes for anti-ATPIF1 antibody
WB 1:500 - 1:1000
IF/ICC 1:50 - 1:100
IP: 0.5ug-4ug antibody for 200 ug-400 ug extracts of whole cells.

Western Blot (WB) of anti-ATPIF1 antibody

Western blot analysis of lysates from wild type(WT) and ATPIF1 knockout (KO) 293T(KO)cells, using [KO Validated] ATPIF1 Rabbit pAb (MBS129181) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200 at 1:10000 dilution.
Lysates/proteins: 25?g per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 1s.
Rabbit Anti-ATPIF1 Polyclonal Antibody – Cat# MBS129181

Immunofluorescence (IF) of anti-ATPIF1 antibody

Immunofluorescence analysis of U2OS cells using [KO Validated] ATPIF1 Rabbit pAb (MBS129181) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining
Rabbit Anti-ATPIF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS129181

Immunoprecipitation (IP) of anti-ATPIF1 antibody

Immunoprecipitation analysis of 300 ug extracts of K-562 cells using 3ug [KO Validated] ATPIF1 Rabbit pAb (MBS129181). Western blot was performed from theimmunoprecipitate using [KO Validated] ATPIF1 Rabbit pAb (MBS129181) at a dilution of 1:3000
Rabbit Anti-ATPIF1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS129181

Immunohistochemistry (IHC) of anti-ATPIF1 antibody

Immunohistochemistry of paraffin-embedded human stomach using ATPIF1 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-ATPIF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS129181

Immunohistochemistry (IHC) of anti-ATPIF1 antibody

Immunohistochemistry of paraffin-embedded human liver cancer using ATPIF1 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-ATPIF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS129181

Immunohistochemistry (IHC) of anti-ATPIF1 antibody

Immunohistochemistry of paraffin-embedded human trachea using ATPIF1 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-ATPIF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS129181

Other Notes

Small volumes of anti-ATPIF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATPIF1 purchase

MBS129181 is an antibody targeting ATPIF1. Also known as ATPIF1 Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATPIF1. It may not necessarily be applicable to this product.

NCBI GI #
12585262
NCBI GeneID
93974
NCBI Accession #
Q9UII2.1
UniProt Primary Accession #
Q9UII2
UniProt Secondary Accession #
Q5JXL8; Q6IAQ7; Q9BSL9
UniProt Related Accession #
Q9UII2
NCBI Official Full Name
ATPase inhibitor, mitochondrial
NCBI Official Synonym Full Names
ATPase inhibitory factor 1
NCBI Official Symbol
ATPIF1
NCBI Official Synonym Symbols
IP; ATPI; ATPIP
UniProt Protein Name
ATPase inhibitor, mitochondrial
UniProt Synonym Protein Names
Inhibitor of F(1)F(o)-ATPase; IF(1); IF1
Protein Family
ATPase inhibitor
UniProt Gene Name
ATPIF1
UniProt Synonym Gene Names
ATPI; IF(1); IF1
UniProt Entry Name
ATIF1_HUMAN
NCBI Protein Information
ATPase inhibitor, mitochondrial; IF1; ATPase inhibitor protein; inhibitor of F(1)F(o)-ATPase; ATP synthase inhibitor protein

NCBI Summary for ATPIF1

This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

UniProt Comments for ATPIF1

ATPIF1: Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Homodimer at a pH value below 6.5, inactive homotetramer above. Belongs to the ATPase inhibitor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Mitochondrial

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: cell surface; mitochondrion; mitochondrial proton-transporting ATP synthase complex

Molecular Function: calmodulin binding; enzyme inhibitor activity; angiostatin binding; protein homodimerization activity; ATPase inhibitor activity; ATPase binding

Biological Process: generation of precursor metabolites and energy; regulation of gene expression; negative regulation of hydrolase activity; negative regulation of endothelial cell proliferation; erythrocyte differentiation; mitochondrial depolarization; angiogenesis; negative regulation of nucleotide metabolic process; protein homooligomerization; heme biosynthetic process; protein homotetramerization
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Research Articles on ATPIF1

1. promotes hepatocellular carcinoma angiogenesis and metastasis by up-regulation of Snai1 and VEGF transcription

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ATPIF1 antibody

 Products by Pathway  Pathway Diagram
 Electron Transport Chain Pathway antibodies  Electron Transport Chain Pathway Diagram

Diseases associated with anti-ATPIF1 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >1 publications with ATPIF1 and Disease Models, Animal

Organs/Tissues associated with anti-ATPIF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with ATPIF1 and Blood
 Brain Antibodies  >1 publications with ATPIF1 and Brain
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