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Nuclear Cap-Binding Protein Subunit 2 (CBP20) Arabidopsis Thaliana Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1291930

MBS1291930 is a recombinant protein targeting CBP20. Also known as Recombinant Arabidopsis thaliana Nuclear cap-binding protein subunit 2 (CBP20). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$940 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nuclear cap-binding protein subunit 2 (CBP20), Recombinant Protein
Full Product Name
Recombinant Arabidopsis thaliana Nuclear cap-binding protein subunit 2 (CBP20)
Product Gene Name
CBP20 recombinant protein
Product Synonym Gene Name
CBP20
Sequence
MASLFKEQAK LSAYRDRRFS GTQEEFDEAL RASTTVYIGN VSFYTTEEQL YELFSRAGEI KKIIMGLDKN TKTPCGFCFV LFYSREDTED AVKYISGTIL DDRPIRVDFD WGFQEGRQWG RGRSGGQVRD EYRTDYDPAR GGYGKLVQKE LEAQRQLVDY GTGSLGAYPQ AAPTNYGNGR RGGGNYGQGG QNRHGRGDYH RKRQRDDDRY GRDNSRRNTD HESRRDTDSD MRPEKNPRFR ESGDSDDDGE DDRKRRS
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
29,609 Da
Sequence Positions
1-257, Full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q9XFD1
Species
Arabidopsis thaliana (Mouse-ear cress)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CBP20 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CBP20. It may not necessarily be applicable to this product.

NCBI GI #
145334719
NCBI GeneID
834443
NCBI Accession #
NP_001078705.1
UniProt Primary Accession #
Q9XFD1
UniProt Related Accession #
Q9XFD1
GenBank Nucleotide #
NM_001085236.2
NCBI Official Full Name
CAP-binding protein 20
NCBI Official Symbol
CBP20
NCBI Official Synonym Symbols
ATCBP20; CAP-binding protein 20; MLN1.12; MLN1_12
UniProt Protein Name
Nuclear cap-binding protein subunit 2
UniProt Synonym Protein Names
20 kDa nuclear cap-binding protein; NCBP 20 kDa subunit; AtCBP20
UniProt Gene Name
CBP20
UniProt Synonym Gene Names
AtCBP20
NCBI Protein Information
CAP-binding protein 20

NCBI Summary for CBP20

Encodes a nuclear cap-binding protein that forms a heterodimeric complex with ABH1 (ATCBP80) and is likely to participate in RNA metabolism. Its mRNA is ubiquitously expressed.Loss of function mutations suggest a role in processing of pri-miRNA and mRNA splicing.

UniProt Comments for CBP20

Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs). The CBC complex is involved in miRNA-mediated RNA interference and is required for primary miRNA processing. In the CBC complex, CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires ABH1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. CBP20 also plays a role in stabilization of ABH1/CBP80 and ABH1/CBP80 localization to the nucleus. Involved in flowering regulation via its interaction with FRIGIDA.
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Research Articles on CBP20

1. we proposed that ethylene regulated phosphorylation of CBP20 is involved in the root growth and one pathway is through the regulation of miR319b and its target MYB33 in roots

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CBP20 recombinant protein

 Reference Product  PubMed Publications
 ABH1 recombinant protein  >7 publications with CBP20 and ABH1
 U1-70K recombinant protein  >1 publications with CBP20 and U1-70K

Pathways associated with CBP20 recombinant protein

 Products by Pathway  Pathway Diagram
 Cap Binding Complex Pathway antibodies  Cap Binding Complex Pathway Diagram
 Cap Binding Complex Pathway antibodies  Cap Binding Complex Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram
 Formation Of The Early Elongation Complex Pathway antibodies  Formation Of The Early Elongation Complex Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Nonsense Mediated Decay (NMD) Enhanced By The Exon Junction Complex (EJC) Pathway antibodies  Nonsense Mediated Decay (NMD) Enhanced By The Exon Junction Complex (EJC) Pathway Diagram
 Nonsense Mediated Decay (NMD) Independent Of The Exon Junction Complex (EJC) Pathway antibodies  Nonsense Mediated Decay (NMD) Independent Of The Exon Junction Complex (EJC) Pathway Diagram
 Nonsense-Mediated Decay (NMD) Pathway antibodies  Nonsense-Mediated Decay (NMD) Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Processing Of Capped Intronless Pre-mRNA Pathway antibodies  Processing Of Capped Intronless Pre-mRNA Pathway Diagram

Organs/Tissues associated with CBP20 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Leaf Antibodies  >8 publications with CBP20 and Leaf
 Flower Antibodies  >4 publications with CBP20 and Flower
 Whole Plant Antibodies  >2 publications with CBP20 and Whole Plant
 Root Antibodies  >2 publications with CBP20 and Root
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