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NUDT21 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS129299
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The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and… More details

$225 / 0.02 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NUDT21, Polyclonal Antibody
Full Product Name
NUDT21 Polyclonal Antibody
Product Gene Name
anti-NUDT21 antibody
Product Synonym Names
CPSF5; CFIM25
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
227
OMIM
604978
3D Structure
ModBase 3D Structure for O43809
Species
Human
Immunogen
Recombinant protein of human NUDT21
Calculated Molecular Weight
26kDa

Product Categories/Family for anti-NUDT21 antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-NUDT21 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-NUDT21 antibody
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50

Western Blot (WB) of anti-NUDT21 antibody

Western blot analysis of extracts of various cell lines, using NUDT21 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Rabbit Anti-NUDT21 Polyclonal Antibody – Cat# MBS129299

Other Notes

Small volumes of anti-NUDT21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NUDT21 purchase

MBS129299 is an antibody targeting NUDT21. Also known as NUDT21 Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NUDT21. It may not necessarily be applicable to this product.

NCBI GI #
74735411
NCBI GeneID
11051
NCBI Accession #
O43809.1
UniProt Primary Accession #
O43809
UniProt Secondary Accession #
Q6IB85; Q6NE84
UniProt Related Accession #
O43809
NCBI Official Full Name
Cleavage and polyadenylation specificity factor subunit 5
NCBI Official Synonym Full Names
nudix (nucleoside diphosphate linked moiety X)-type motif 21
NCBI Official Symbol
NUDT21
NCBI Official Synonym Symbols
CPSF5; CFIM25
UniProt Protein Name
Cleavage and polyadenylation specificity factor subunit 5
UniProt Synonym Protein Names
Cleavage and polyadenylation specificity factor 25 kDa subunit; CFIm25; CPSF 25 kDa subunit; Nucleoside diphosphate-linked moiety X motif 21; Nudix motif 21; Pre-mRNA cleavage factor Im 25 kDa subunit
Protein Family
Nudix hydrolase
UniProt Gene Name
NUDT21
UniProt Synonym Gene Names
CFIM25; CPSF25; CPSF5; CFIm25; CPSF 25 kDa subunit; Nudix motif 21
UniProt Entry Name
CPSF5_HUMAN
NCBI Protein Information
cleavage and polyadenylation specificity factor subunit 5; nudix motif 21; CPSF 25 kDa subunit; pre-mRNA cleavage factor Im (25kD); cleavage factor Im complex 25 kDa subunit; pre-mRNA cleavage factor Im, 25kD subunit; pre-mRNA cleavage factor Im 25 kDa subunit; nucleoside diphosphate-linked moiety X motif 21; cleavage and polyadenylation specific factor 5, 25 kDa; cleavage and polyadenylation specific factor 5, 25 kD subunit; cleavage and polyadenylation specificity factor 25 kDa subunit

UniProt Comments for NUDT21

CFIM25: Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export. Belongs to the Nudix hydrolase family. CPSF5 subfamily.

Protein type: Spliceosome; RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: nucleoplasm; centrosome; nucleus; paraspeckles; mRNA cleavage factor complex

Molecular Function: mRNA binding; protein binding; protein homodimerization activity; hydrolase activity; RNA binding; histone deacetylase binding; AU-rich element binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; RNA splicing; gene expression; mRNA polyadenylation; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription; protein tetramerization
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Research Articles on NUDT21

1. Structure of a CFI(m)25/CFI(m)68 RRM heterotetramer and biochemical data indicated that CFIm25 specifically recognized two UGUA elements, CFIm68 facilitates looping of the intervening RNA. CFIm-mediated RNA looping may regulate alternative polyadnylation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NUDT21 antibody

 Reference Product  PubMed Publications
 CPSF6 antibody  >2 publications with NUDT21 and CPSF6

Pathways associated with anti-NUDT21 antibody

 Products by Pathway  Pathway Diagram
 Cleavage Of Growing Transcript In The Termination Region Pathway antibodies  Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Processing Of Capped Intronless Pre-mRNA Pathway antibodies  Processing Of Capped Intronless Pre-mRNA Pathway Diagram
 Processing Of Intronless Pre-mRNAs Pathway antibodies  Processing Of Intronless Pre-mRNAs Pathway Diagram
 RNA Polymerase II Transcription Pathway antibodies  RNA Polymerase II Transcription Pathway Diagram
 RNA Polymerase II Transcription Termination Pathway antibodies  RNA Polymerase II Transcription Termination Pathway Diagram
 MRNA 3'-end Processing Pathway antibodies  MRNA 3'-end Processing Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram

Diseases associated with anti-NUDT21 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >1 publications with NUDT21 and Immune System Diseases

Organs/Tissues associated with anti-NUDT21 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with NUDT21 and Testis
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