Rabbit Anti-PUF60 Polyclonal Antibody – Cat# MBS129352

Western blot analysis of extracts of various cell lines, using PUF60 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

PUF60 Antibody – Rabbit Polyclonal

Name: PUF60 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS129352
Price: 360 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS129352

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

PUF60, Polyclonal Antibody

Full Product Name

PUF60 Polyclonal Antibody

Product Gene Name

anti-PUF60 antibody

Product Synonym Names

FIR; VRJS; RoBPI; SIAHBP1

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

559

OMIM

gene 615583

3D Structure

ModBase 3D Structure for Q9UHX1

Species

Human

Immunogen

Recombinant protein of human PUF60

Calculated Molecular Weight

60kDa

Product Categories/Family for anti-PUF60 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-PUF60 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-PUF60 antibody

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Other Notes

Small volumes of anti-PUF60 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PUF60 purchase

MBS129352 is an antibody targeting PUF60. Also known as PUF60 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PUF60. It may not necessarily be applicable to this product.

NCBI GI #

74761960

NCBI GeneID

22827

NCBI Accession #

Q9UHX1.1

UniProt Primary Accession #

Q9UHX1

UniProt Secondary Accession #

Q969E7; Q96D94; Q96H63; Q99628; Q9NZA0; Q9UJY7; A8K8K8

UniProt Related Accession #

Q9UHX1

NCBI Official Full Name

Poly(U)-binding-splicing factor PUF60

NCBI Official Synonym Full Names

poly-U binding splicing factor 60KDa

NCBI Official Symbol

PUF60

NCBI Official Synonym Symbols

FIR; VRJS; RoBPI; SIAHBP1

UniProt Protein Name

Poly(U)-binding-splicing factor PUF60

UniProt Synonym Protein Names

60 kDa poly(U)-binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1

Protein Family

Poly(U)-binding-splicing factor

UniProt Gene Name

PUF60

UniProt Synonym Gene Names

FIR; ROBPI; SIAHBP1; FBP-interacting repressor; RoBP1; Siah-BP1

UniProt Entry Name

PUF60_HUMAN

NCBI Protein Information

poly(U)-binding-splicing factor PUF60; poly(U)-binding-splicing factor PUF60; Siah binding protein 1; FBP interacting repressor; Ro ribonucleoprotein-binding protein 1; pyrimidine tract binding splicing factor; FUSE-binding protein-interacting repressor

UniProt Comments for PUF60

FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding; Spliceosome; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; Golgi apparatus; mitochondrion; ribonucleoprotein complex; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: identical protein binding; protein binding; DNA binding; nucleotide binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; RNA splicing; mRNA processing

Disease: Verheij Syndrome

Research Articles on PUF60

1. The interaction between SAP155 and FIR/FIRDeltaexon2 not only integrates cell-cycle progression and c-Myc transcription by modifying P27 and P89 expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PUF60 antibody

Products by Pathway	Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram

Diseases associated with anti-PUF60 antibody

Disease Name	Pubmed Publications
Ovarian Diseases Antibodies	>1 publications with PUF60 and Ovarian Diseases
Neoplasm Metastasis Antibodies	>1 publications with PUF60 and Neoplasm Metastasis

Organs/Tissues associated with anti-PUF60 antibody

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>1 publications with PUF60 and Heart
Stomach Antibodies	>1 publications with PUF60 and Stomach
Lymph Antibodies	>1 publications with PUF60 and Lymph
Lymph Node Antibodies	>1 publications with PUF60 and Lymph Node
Intestine Antibodies	>1 publications with PUF60 and Intestine
Ovary Antibodies	>1 publications with PUF60 and Ovary