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H2AFZ Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS129404
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core… More details

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Product Overview

Product Name
H2AFZ, Polyclonal Antibody
Full Product Name
H2AFZ Polyclonal Antibody
Product Gene Name
anti-H2AFZ antibody
Product Synonym Names
H2AZ; H2A.z; H2A/z; H2A.Z-1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
128
OMIM
142763
3D Structure
ModBase 3D Structure for P0C0S5
Species
Human
Immunogen
Recombinant protein of human H2AFZ
Calculated Molecular Weight
14kDa

Product Categories/Family for anti-H2AFZ antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-H2AFZ antibody
Western Blot (WB)
Application Notes for anti-H2AFZ antibody
WB: 1:500-1:2000

Western Blot (WB) of anti-H2AFZ antibody

Western blot analysis of extracts of Jurkat cells, using H2AFZ antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Rabbit Anti-H2AFZ Polyclonal Antibody – Cat# MBS129404

Other Notes

Small volumes of anti-H2AFZ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for H2AFZ purchase

MBS129404 is an antibody targeting H2AFZ. Also known as H2AFZ Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for H2AFZ. It may not necessarily be applicable to this product.

NCBI GI #
83288408
NCBI GeneID
3015
NCBI Accession #
P0C0S5.2
UniProt Primary Accession #
P0C0S5
UniProt Secondary Accession #
P17317; Q6I9U0; B2RD56
UniProt Related Accession #
P0C0S5
NCBI Official Full Name
Histone H2A.Z
NCBI Official Synonym Full Names
H2A histone family, member Z
NCBI Official Symbol
H2AFZ
NCBI Official Synonym Symbols
H2AZ; H2A.z; H2A/z; H2A.Z-1
UniProt Protein Name
Histone H2A.Z
UniProt Gene Name
H2AFZ
UniProt Synonym Gene Names
H2AZ; H2A/z
UniProt Entry Name
H2AZ_HUMAN
NCBI Protein Information
histone H2A.Z; H2AZ histone

UniProt Comments for H2AFZ

H2AZ: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP. Belongs to the histone H2A family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: Barr body; nuclear heterochromatin; nucleosome; nucleus

Molecular Function: protein binding; nucleosomal DNA binding; chromatin DNA binding; protein heterodimerization activity

Biological Process: positive regulation of transcription from RNA polymerase II promoter
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Research Articles on H2AFZ

1. A mutational analysis revealed that the amino-acid difference at position 38 is at least partially responsible for the structural polymorphism in the L1 loop region of H2A.Z.1 and H2A.Z.2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-H2AFZ antibody

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 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram

Diseases associated with anti-H2AFZ antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >2 publications with H2AFZ and Carcinoma
 Neoplasms, Experimental Antibodies  >2 publications with H2AFZ and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >2 publications with H2AFZ and Adenocarcinoma
 Abnormalities, Drug-Induced Antibodies  >1 publications with H2AFZ and Abnormalities, Drug-Induced
 Weight Gain Antibodies  >1 publications with H2AFZ and Weight Gain
 Prostatic Diseases Antibodies  >1 publications with H2AFZ and Prostatic Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with H2AFZ and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >1 publications with H2AFZ and Liver Diseases
 Cell Transformation, Neoplastic Antibodies  >1 publications with H2AFZ and Cell Transformation, Neoplastic
 Prostatic Neoplasms Antibodies  >1 publications with H2AFZ and Prostatic Neoplasms

Organs/Tissues associated with anti-H2AFZ antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with H2AFZ and Brain
 Vascular Antibodies  >1 publications with H2AFZ and Vascular
 Connective Tissue Antibodies  >1 publications with H2AFZ and Connective Tissue
 Placenta Antibodies  >1 publications with H2AFZ and Placenta
 Lung Antibodies  >1 publications with H2AFZ and Lung
 Bone Marrow Antibodies  >1 publications with H2AFZ and Bone Marrow
 Prostate Antibodies  >1 publications with H2AFZ and Prostate
 Embryonic Tissue Antibodies  >1 publications with H2AFZ and Embryonic Tissue
 Liver Antibodies  >1 publications with H2AFZ and Liver
 Ovary Antibodies  >1 publications with H2AFZ and Ovary
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