Rabbit Anti-RPS14 Polyclonal Antibody – Cat# MBS129537

Western blot analysis of extracts of HeLa cells, using RPS14 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

RPS14 Antibody – Rabbit Polyclonal

Name: RPS14 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS129537
Price: 360 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS129537

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

RPS14, Polyclonal Antibody

Full Product Name

RPS14 Polyclonal Antibody

Product Gene Name

anti-RPS14 antibody

Product Synonym Names

S14; EMTB

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

151

OMIM

130620

3D Structure

ModBase 3D Structure for P62263

Species

Human

Immunogen

Recombinant protein of human RPS14

Calculated Molecular Weight

16kDa

Product Categories/Family for anti-RPS14 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-RPS14 antibody

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Application Notes for anti-RPS14 antibody

WB: 1:1000-1:2000
IHC: 1:100-1:200
IF: 1:50-1:100

Other Notes

Small volumes of anti-RPS14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPS14 purchase

MBS129537 is an antibody targeting RPS14. Also known as RPS14 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RPS14. It may not necessarily be applicable to this product.

NCBI GI #

50403752

NCBI GeneID

6208

NCBI Accession #

P62263.3

UniProt Primary Accession #

P62263

UniProt Secondary Accession #

P06366; Q5BJI0; B2R5G5; D3DQG5

UniProt Related Accession #

P62263

NCBI Official Full Name

40S ribosomal protein S14

NCBI Official Synonym Full Names

ribosomal protein S14

NCBI Official Symbol

RPS14

NCBI Official Synonym Symbols

S14; EMTB

UniProt Protein Name

40S ribosomal protein S14

Protein Family

30S ribosomal protein

UniProt Gene Name

RPS14

UniProt Entry Name

RS14_HUMAN

NCBI Protein Information

40S ribosomal protein S14; emetine resistance

UniProt Comments for RPS14

RPS14: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Protein type: RNA-binding; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 5q31-q33

Cellular Component: focal adhesion; membrane; mitochondrion; cytoplasm; nucleolus; cytosol

Molecular Function: structural constituent of ribosome; RNA binding; translation regulator activity; mRNA 5'-UTR binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; viral reproduction; translation; viral infectious cycle; negative regulation of transcription from RNA polymerase II promoter; translational termination; maturation of SSU-rRNA; regulation of translation; cellular protein metabolic process; translational elongation; translational initiation; mRNA catabolic process, nonsense-mediated decay; ribosomal small subunit assembly and maintenance; erythrocyte differentiation; gene expression; viral transcription

Disease: Chromosome 5q Deletion Syndrome

Research Articles on RPS14

1. Partial silencing of RPS14 inhibits the proliferation of SKM-1, an acute myeloid leukemia cell line, and RPS14 negatively regulates p53 activation in SKM-1 cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPS14 antibody

Reference Product	PubMed Publications
RPS3 antibody	>7 publications with RPS14 and RPS3
RPL11 antibody	>6 publications with RPS14 and RPL11
RPS29 antibody	>3 publications with RPS14 and RPS29
RPL35A antibody	>3 publications with RPS14 and RPL35A
RPS16 antibody	>3 publications with RPS14 and RPS16
RPS13 antibody	>2 publications with RPS14 and RPS13
RPL9 antibody	>1 publications with RPS14 and RPL9
RPL7A antibody	>1 publications with RPS14 and RPL7A
RPS20 antibody	>1 publications with RPS14 and RPS20
RPS23 antibody	>1 publications with RPS14 and RPS23

Pathways associated with anti-RPS14 antibody

Products by Pathway	Pathway Diagram
Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies	Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
Cap-dependent Translation Initiation Pathway antibodies	Cap-dependent Translation Initiation Pathway Diagram
Cytoplasmic Ribosomal Proteins Pathway antibodies	Cytoplasmic Ribosomal Proteins Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Eukaryotic Translation Elongation Pathway antibodies	Eukaryotic Translation Elongation Pathway Diagram
Eukaryotic Translation Initiation Pathway antibodies	Eukaryotic Translation Initiation Pathway Diagram
Eukaryotic Translation Termination Pathway antibodies	Eukaryotic Translation Termination Pathway Diagram
Formation Of A Pool Of Free 40S Subunits Pathway antibodies	Formation Of A Pool Of Free 40S Subunits Pathway Diagram
Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway antibodies	Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway Diagram
GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies	GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram

Diseases associated with anti-RPS14 antibody

Disease Name	Pubmed Publications
5q- syndrome Antibodies	>46 publications with RPS14 and 5q- syndrome
Congenital Abnormalities Antibodies	>8 publications with RPS14 and Congenital Abnormalities
Disease Models, Animal Antibodies	>4 publications with RPS14 and Disease Models, Animal
Neurobehavioral Manifestations Antibodies	>2 publications with RPS14 and Neurobehavioral Manifestations
Brain Diseases Antibodies	>1 publications with RPS14 and Brain Diseases
Lung Diseases Antibodies	>1 publications with RPS14 and Lung Diseases
Cell Transformation, Neoplastic Antibodies	>1 publications with RPS14 and Cell Transformation, Neoplastic
Liver Diseases Antibodies	>1 publications with RPS14 and Liver Diseases
Hyperplasia Antibodies	>1 publications with RPS14 and Hyperplasia
Liver Neoplasms Antibodies	>1 publications with RPS14 and Liver Neoplasms

Organs/Tissues associated with anti-RPS14 antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>23 publications with RPS14 and Bone
Bone Marrow Antibodies	>23 publications with RPS14 and Bone Marrow
Blood Antibodies	>19 publications with RPS14 and Blood
Ovary Antibodies	>6 publications with RPS14 and Ovary
Brain Antibodies	>3 publications with RPS14 and Brain
Heart Antibodies	>3 publications with RPS14 and Heart
Mammary Gland Antibodies	>1 publications with RPS14 and Mammary Gland
Liver Antibodies	>1 publications with RPS14 and Liver
Skin Antibodies	>1 publications with RPS14 and Skin
Lung Antibodies	>1 publications with RPS14 and Lung