• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Retinoid Isomerohydrolase (RPE65) Mouse Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1296451

This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated,… More details

$1340 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Retinoid isomerohydrolase (Rpe65), Recombinant Protein
Full Product Name
Recombinant Mouse Retinoid isomerohydrolase (Rpe65)
Product Gene Name
Rpe65 recombinant protein
Product Synonym Gene Name
Rpe65
Sequence
SIQIEHPAGG YKKLFETVEE LSSPLTAHVT GRIPLWLTGS LLRCGPGLFE VGSEPFYHLF DGQALLHKFD FKEGHVTYHR RFIRTDAYVR AMTEKRIVIT EFGTCAFPDP CKNIFSRFFS YFKGVEVTDN ALVNIYPVGE DYYACTETNF ITKINPETLE TIKQVDLCNY ISVNGATAHP HIESDGTVYN IGNCFGKNFT VAYNIIKIPP LKADKEDPIN KSEVVVQFPC SDRFKPSYVH SFGLTPNYIV FVETPVKINL FKFLSSWSLW GANYMDCFES NESMGVWLHV ADKKRRKYFN NKYRTSPFNL FHHINTYEDN GFLIVDLCCW KGFEFVYNYL YLANLRENWE EVKRNAMKAP QPEVRRYVLP LTIDKVDTGR NLVTLPHTTA TATLRSDETI WLEPEVLFSG PRQAFEFPQI NYQKFGGKPY TYAYGLGLNH FVPDKLCKLN VKTKEIWMWQ EPDSYPSEPI FVSQPDALEE DDGVVLSVVV SPGAGQKPAY LLVLNAKDLS EIARAEVETN IPVTFHGLFK RS
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
61,085 Da
Sequence Positions
2-533, Full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q91ZQ5
Species
Mouse
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of Rpe65 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rpe65 purchase

MBS1296451 is a recombinant protein targeting Rpe65. Also known as Recombinant Mouse Retinoid isomerohydrolase (Rpe65). Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for Rpe65. It may not necessarily be applicable to this product.

NCBI GI #
147902089
NCBI GeneID
19892
NCBI Accession #
NP_084263.2
UniProt Primary Accession #
Q91ZQ5
UniProt Secondary Accession #
Q8VHP2; A1L3D1; E9QNS6; H9KUX9
UniProt Related Accession #
Q91ZQ5
GenBank Nucleotide #
NM_029987.2
NCBI Official Full Name
retinoid isomerohydrolase
NCBI Official Synonym Full Names
retinal pigment epithelium 65
NCBI Official Symbol
Rpe65
NCBI Official Synonym Symbols
LCA2; RP20; rd12; 65kDa; Mord1; A930029L06Rik
UniProt Protein Name
Retinoid isomerohydrolase
UniProt Synonym Protein Names
All-trans-retinyl-palmitate hydrolase; Meso-zeaxanthin isomerase (EC:5.3.3.-
UniProt Gene Name
Rpe65
NCBI Protein Information
retinoid isomerohydrolase

UniProt Comments for Rpe65

Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore (PubMed:15765048, PubMed:9843205, PubMed:23407971, PubMed:28500718). Essential for the production of 11-cis retinal for both rod and cone photoreceptors (PubMed:17251447). Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT ().
Show more

Research Articles on Rpe65

1. Study describes a mouse model of Leber's Congenital Amaurosis type 2 with mutation in Rpe65 gene. Functional and biochemical studies confirm that vitamin A metabolism and visual processing are disrupted in this model.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Rpe65 recombinant protein

 Reference Product  PubMed Publications
 Lrat recombinant protein  >47 publications with Rpe65 and Lrat
 Crb1 recombinant protein  >34 publications with Rpe65 and Crb1
 Rho recombinant protein  >21 publications with Rpe65 and Rho
 Rdh12 recombinant protein  >14 publications with Rpe65 and Rdh12
 Abca4 recombinant protein  >9 publications with Rpe65 and Abca4
 Rdh5 recombinant protein  >5 publications with Rpe65 and Rdh5
 Rlbp1 recombinant protein  >4 publications with Rpe65 and Rlbp1
 Rdh11 recombinant protein  >2 publications with Rpe65 and Rdh11

Pathways associated with Rpe65 recombinant protein

 Products by Pathway  Pathway Diagram
 Retinol Metabolism Pathway antibodies  Retinol Metabolism Pathway Diagram
 Retinol Metabolism Pathway antibodies  Retinol Metabolism Pathway Diagram
 Retinol Metabolism Pathway antibodies  Retinol Metabolism Pathway Diagram

Diseases associated with Rpe65 recombinant protein

 Disease Name  Pubmed Publications
 Leber Congenital Amaurosis Antibodies  >158 publications with Rpe65 and Leber Congenital Amaurosis
 Eye Diseases, Hereditary Antibodies  >131 publications with Rpe65 and Eye Diseases, Hereditary
 Blindness Antibodies  >116 publications with Rpe65 and Blindness
 Disease Models, Animal Antibodies  >87 publications with Rpe65 and Disease Models, Animal
 Retinal Dystrophies Antibodies  >66 publications with Rpe65 and Retinal Dystrophies
 Retinitis Pigmentosa Antibodies  >62 publications with Rpe65 and Retinitis Pigmentosa
 Macular Degeneration Antibodies  >54 publications with Rpe65 and Macular Degeneration
 Optic Atrophy, Hereditary, Leber Antibodies  >32 publications with Rpe65 and Optic Atrophy, Hereditary, Leber
 Nerve Degeneration Antibodies  >16 publications with Rpe65 and Nerve Degeneration
 Brain Diseases Antibodies  >7 publications with Rpe65 and Brain Diseases

Organs/Tissues associated with Rpe65 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >405 publications with Rpe65 and Eye
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000