• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Lysine-Specific Demethylase REF6 (REF6) Arabidopsis Thaliana Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1322612

MBS1322612 is a recombinant protein targeting REF6. Also known as Recombinant Arabidopsis thaliana Lysine-specific demethylase REF6 (REF6) , partial. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$1075 / 0.05 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Lysine-specific demethylase REF6 (REF6), Recombinant Protein
Full Product Name
Recombinant Arabidopsis thaliana Lysine-specific demethylase REF6 (REF6) , partial
Product Gene Name
REF6 recombinant protein
Product Synonym Gene Name
REF6
Sequence
LPVAPEFRPT LAEFQDPIAY ILKIEEEASR YGICKILPPL PPPSKKTSIS NLNRSLAARA AARVRDGGFG ACDYDGGPTF ATRQQQIGFC PRKQRPVQRP VWQSGEEYSF GEFEFKAKNF EKNYLKKCGK KSQLSALEIE TLYWRATVDK PFSVEYANDM PGSAFIPLSL AAARRRESGG EGGTVGETAW NMRAMSRAEG SLLKFMKEEI PGVTSPMVYV AMMFSWFAWH VEDHDLHSLN YLHMGAGKTW YGVPKDAALA FEEVVRVHGY GEELNPLVTF STLGEKTTVM SPEVFVKAGI PCCRLVQNPG EFVVTFPGAY HSGFSHGFNF GEASNIATPE WLRMAKDAAI RRAAIN
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
152,629 Da
Sequence Positions
17-372. Partial
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q9STM3
Species
Arabidopsis thaliana (Mouse-ear cress)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of REF6 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for REF6. It may not necessarily be applicable to this product.

NCBI GI #
79586698
NCBI GeneID
824002
NCBI Accession #
NP_680116.2
UniProt Primary Accession #
Q9STM3
UniProt Related Accession #
Q9STM3
GenBank Nucleotide #
NM_148863.4
NCBI Official Full Name
relative of early flowering 6
NCBI Official Symbol
REF6
NCBI Official Synonym Symbols
JMJ12; Jumonji domain-containing protein 12; relative of early flowering 6
UniProt Protein Name
Lysine-specific demethylase REF6
UniProt Synonym Protein Names
Jumonji domain-containing protein 12
Protein Family
Lysine-specific demethylase
UniProt Gene Name
REF6
NCBI Protein Information
relative of early flowering 6

NCBI Summary for REF6

Relative of Early Flowering 6 (REF6) encodes a Jumonji N/C and zinc finger domain-containing protein that acts as a positive regulator of flowering in an FLC-dependent pathway. REF6 mutants have hyperacetylation of histone H4 at the FLC locus. REF6 interacts with BES1 in a Y2H assay and in vitro. REF6 may play a role in brassinoteroid signaling by affecting histone methylation in the promoters of BR-responsive genes. It is most closely related to the JHDM3 subfamily of JmjN/C proteins.
Show more

UniProt Comments for REF6

Histone demethylase that demethylates 'Lys-27' (H3K27me) of histone H3. Demethylates both tri- (H3K27me3) and di-methylated (H3K27me2) H3K27me (PubMed:21642989, PubMed:27111035). Demethylates also H3K4me3/2 and H3K36me3/2 in an in vitro assay (PubMed:20711170). Involved in the transcriptional regulation of hundreds of genes regulating developmental patterning and responses to various stimuli (PubMed:18467490). Binds DNA via its four zinc fingers in a sequence-specific manner, 5'-CTCTGYTY-3', to promote the demethylation of H3K27me3 and the regulation of organ boundary formation (PubMed:27111034, PubMed:27111035). Involved in the regulation of flowering time by repressing FLOWERING LOCUS C (FLC) expression (PubMed:15377760). Interacts with the NF-Y complexe to regulate SOC1 (PubMed:25105952). Mediates the recruitment of BRM to its target loci (PubMed:27111034).
Show more

Research Articles on REF6

1. Genome-wide analyses showed that REF6 colocalizes with BRM at many genomic sites with the CTCTGYTY motif. Loss of REF6 results in decreased BRM occupancy at BRM-REF6 co-targets

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with REF6 recombinant protein

 Reference Product  PubMed Publications
 BES1 recombinant protein  >2 publications with REF6 and BES1
 FLC recombinant protein  >1 publications with REF6 and FLC

Pathways associated with REF6 recombinant protein

 Products by Pathway  Pathway Diagram
 Flowering Time Pathway antibodies  Flowering Time Pathway Diagram
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000