Banana Bunchy Top Virus DNA-R Recombinant Protein – Cat# MBS1329057

Master Replication Protein (DNA-R) Banana Bunchy Top Virus Recombinant Protein

Name: DNA-R Recombinant Protein; Banana Bunchy Top Virus; MyBioSource
Brand: MyBioSource
SKU: MBS1329057
Price: 975 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1329057

MBS1329057 is a recombinant protein targeting DNA-R. Also known as Recombinant Banana bunchy top virus Master replication protein (DNA-R). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$975 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Master replication protein (DNA-R), Recombinant Protein

Full Product Name

Recombinant Banana bunchy top virus Master replication protein (DNA-R)

Product Gene Name

DNA-R recombinant protein

Product Synonym Gene Name

DNA-R

Sequence

MARYVVCWMF TINNPTTLPV MRDEIKYMVY QVERGQEGTR HVQGYVEMKR RSSLKQMRGF FPGAHLEKRK GSQEEARSYC MKEDTRIEGP FEFGSFKLSC NDNLFDVIQD MRETHKRPLE YLYDCPNTFD RSKDTLYRVQ AEMNKTKAMN SWRTSFSAWT SEVENIMAQP CHRRIIWVYG PNGGEGKTTY AKHLMKTRNA FYSPGGKSLD ICRLYNYEDI VIFDIPRCKE DYLNYGLLEE FKNGIIQSGK YEPVLKIVEY VEVIVMANFL PKEGIFSEDR IKLVSC

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Molecular Weight

33,604 Da

Sequence Positions

1-286, Full length protein

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

3D Structure

ModBase 3D Structure for Q86567

Species

Banana bunchy top virus (isolate Autralia) (BBTV)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DNA-R recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DNA-R. It may not necessarily be applicable to this product.

NCBI GI #

19744937

NCBI GeneID

963871

NCBI Accession #

NP_604483.1

UniProt Primary Accession #

Q86567

GenBank Nucleotide #

NC_003479.1

NCBI Official Full Name

replication initiation protein

NCBI Official Symbol

BBTVs1gp1

UniProt Protein Name

Master replication protein

Protein Family

Master replication protein

UniProt Gene Name

DNA-R

UniProt Synonym Gene Names

C1; M-Rep

NCBI Protein Information

replication initiation protein

UniProt Comments for DNA-R

Essential for the replication of all genomic viral ssDNA (trans-replication). The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-A[GT]TATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities ().

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.