DNA-Directed RNA Polymerase III Subunit RPC3 (POLR3C) Human Recombinant Protein

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other mbers of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation.Wang Z., Roeder R.G.Genes Dev. 11:1315-1326(1997) Identification of an immunodominant epitope on RNA polymerase III recognized by systemic sclerosis sera application to enzyme-linked immunosorbent assay.Kuwana M., Kimura K., Kawakami Y.Arthritis Rheum. 46:2742-2747(2002) Genomic structure of human RNA polymerase III subunit (RPC62) .Totaro A. The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity.Hsieh Y.-J., Kundu T.K., Wang Z., Kovelman R., Roeder R.G.Mol. Cell. Biol. 19:7697-7704(1999) Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits.Hu P., Wu S., Sun Y., Yuan C.-C., Kobayashi R., Myers M.P., Hernandez N.Mol. Cell. Biol. 22:8044-8055(2002) A quantitative atlas of mitotic phosphorylation.Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway.Chiu Y.-H., Macmillan J.B., Chen Z.J.Cell 138:576-591(2009) RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate.Ablasser A., Bauernfeind F., Hartmann G., Latz E., Fitzgerald K.A., Hornung V.Nat. Immunol. 10:1065-1072(2009) Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.Sci. Signal. 4:RS3-RS3(2011)

Small volumes of POLR3C recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1337159 is a recombinant protein targeting POLR3C. Also known as Recombinant Human DNA-directed RNA polymerase III subunit RPC3. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.