Histamine (HIS) ELISA Kit for Rat

Name: Rat HIS ELISA Kit; Histamine; MyBioSource
Brand: MyBioSource
SKU: MBS013382
Price: 670 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS013382

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of HIS (hereafter termed this analyte) in undiluted original Rat serum, plasma and tissue homogenate samples. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Product Name

Histamine, ELISA Kit

Full Product Name

Rat Histamine ELISA Kit

Product Gene Name

HIS elisa kit

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma and Tissue Homogenate

Detection Range

1.25 ng/ml - 40 ng/ml.

Sensitivity

0.1 ng/ml.

Specificity

No significant cross-reactivity or interference between this analyte and analogues is observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

40,098 Da

OMIM

142703

3D Structure

ModBase 3D Structure for P25021

Inter-assay

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Other Notes

Small volumes of HIS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIS purchase

MBS013382 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Histamine, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HIS. The ELISA analytical biochemical technique of the MBS013382 kit is based on HIS antibody-HIS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HIS. It may not necessarily be applicable to this product.

NCBI GI #

197085610

NCBI GeneID

3274

NCBI Accession #

NP_001124527.1

UniProt Primary Accession #

P25021

UniProt Secondary Accession #

Q14464; Q7Z5R9; B5BUP7

UniProt Related Accession #

P25021

GenBank Nucleotide #

NM_001131055.1

NCBI Official Full Name

histamine H2 receptor isoform 1

NCBI Official Synonym Full Names

histamine receptor H2

NCBI Official Symbol

HRH2

NCBI Official Synonym Symbols

H2R

UniProt Protein Name

Histamine H2 receptor

UniProt Synonym Protein Names

Gastric receptor I

Protein Family

Histamine H1 receptor

UniProt Gene Name

HRH2

UniProt Synonym Gene Names

H2R; HH2R

UniProt Entry Name

HRH2_HUMAN

NCBI Protein Information

histamine H2 receptor; HH2R; gastric receptor 1; gastric receptor I

NCBI Summary for HIS

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

UniProt Comments for HIS

H2R: The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: histamine receptor activity

Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; gland development; gastrin-induced gastric acid secretion; histamine-induced gastric acid secretion; gut development; positive regulation of vasoconstriction; visual learning; regulation of synaptic plasticity; immune response; memory

Research Articles on HIS

1. But carriers of one or three copies of HRH1 (5% of individuals), HRH2 (1.1%) and HRH4 genes (0.9%) were also identified.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HIS elisa kit

Reference Product	PubMed Publications
JUN elisa kit	>4 publications with HIS and JUN
HDC elisa kit	>2 publications with HIS and HDC
ATP4A elisa kit	>2 publications with HIS and ATP4A

Pathways associated with HIS elisa kit

Products by Pathway	Pathway Diagram
Amine Ligand-binding Receptors Pathway antibodies	Amine Ligand-binding Receptors Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
Gastric Acid Secretion Pathway antibodies	Gastric Acid Secretion Pathway Diagram
Gastric Acid Secretion Pathway antibodies	Gastric Acid Secretion Pathway Diagram

Diseases associated with HIS elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>8 publications with HIS and Neoplasms
Drug Hypersensitivity Antibodies	>3 publications with HIS and Drug Hypersensitivity
Inflammation Antibodies	>2 publications with HIS and Inflammation
Pain Antibodies	>2 publications with HIS and Pain
Seizures Antibodies	>2 publications with HIS and Seizures
Neoplasms, Experimental Antibodies	>2 publications with HIS and Neoplasms, Experimental
Ulcer Antibodies	>1 publications with HIS and Ulcer
Peptic Ulcer Antibodies	>1 publications with HIS and Peptic Ulcer

Organs/Tissues associated with HIS elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>3 publications with HIS and Brain
Skin Antibodies	>2 publications with HIS and Skin